高铁酸钾对水体中黏菌素耐药基因mcr-1的作用

doi:10.12160/j.issn.1672-5190.2024.06.002

摘要/Abstract

摘要： [目的] 探讨高铁酸钾对水体中质粒介导的黏菌素耐药基因mcr-1的作用。[方法] 采用4因素3水平正交试验法,通过实时荧光定量PCR测定水体中mcr-1基因的绝对丰度,以mcr-1基因消减率为指标,考察不同因素及水平[高铁酸钾（A）：7.09、14.17、21.26 mg/L;反应温度（B）：40、50、60 ℃;反应时间（C）：5、15、30 min;反应pH值（D）：5、7、9）]对mcr-1基因拷贝数的影响。[结果] 4个因素中,对mcr-1基因绝对丰度影响最大的是高铁酸钾用量,其次是反应pH值和反应时间,影响最小的是反应温度;对mcr-1基因消减效果最佳的水平组合为A₃B₃C₁D₃,即采用21.26 mg/L高铁酸钾（以Fe计为6 mg/L）,在反应pH值为5、反应温度为60 ℃的条件下处理15 min,该条件下mcr-1基因的消减率为55%。但在某些因素水平组合下,mcr-1基因会出现拷贝数增加的现象。[结论] 高铁酸钾可通过改变DNA浓度来影响mcr-1基因含量。在适当条件下,高铁酸钾可消减水体中的mcr-1基因;但在某些因素水平组合下,mcr-1基因复制增加。

关键词: mcr-1, 高铁酸钾, 耐药基因, 正交试验, 环境

Abstract: [Objective] The aim of this study was to investigate the effect of potassium ferrate on plasmid-mediated colistin resistance gene mcr-1. [Method] The absolute abundance of mcr-1 gene in water was determined by real-time fluorescence quantitative PCR using four-factor and three-level orthogonal method. The elimination rate of mcr-1 gene was used as an index to investigate different factors and levels （potassium ferrate: 7.09 mg/L, 14.17 mg/L, 21.26 mg/L; temperature: 40 ℃, 50 ℃, 60 ℃; reaction time: 5 min, 15 min, 30 min; pH: 5, 7, 9） on mcr-1 gene content. [Result] Among the four factors, the greatest influence on the absolute abundance of mcr-1 gene was the dosage of potassium ferrate, followed by the reaction pH and reaction time, and the least influence was the reaction temperature. The level combination with the best effect on mcr-1 gene ablation was A₃B₃C₁D₃, which was treated with 21.26 mg/L potassium ferrate （6 mg/L in Fe） for 15 min at a reaction pH of 5 and a reaction temperature of 60 ℃, and the ablation rate of the mcr-1 gene under this condition was 55%. However, the copy number of mcr-1 gene increased under certain combinations of factors. [Conclusion] Potassium ferrate could affect mcr-1 gene levels by altering DNA concentrations. Under appropriate conditions, potassium ferrate abrogated the mcr-1 gene in the water. However, under certain combinations of factor levels, mcr-1 gene replication will increase.

Key words: mcr-1, potassium ferrate, resistance gene, orthogonal test, environment

中图分类号:

王旺, 王忠, 吴俊杰, 杨利利, 邓芳, 黎望, 王立琦. 高铁酸钾对水体中黏菌素耐药基因mcr-1的作用[J]. 畜牧与饲料科学, 2024, 45(6): 12-18.

WANG Wang, WANG Zhong, WU Junjie, YANG Lili, DENG Fang, LI Wang, WANG Liqi. Effect of Potassium Ferrate on Colistin Resistance Gene mcr-1 in Water[J]. Animal Husbandry and Feed Science, 2024, 45(6): 12-18.

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