畜牧与饲料科学 ›› 2024, Vol. 45 ›› Issue (6): 25-29.doi: 10.12160/j.issn.1672-5190.2024.06.004

• 食品科学 • 上一篇    下一篇

不同杀菌条件对酸乳清饮料中微生物的影响

郑伟1, 李子健1, 张园园1, 王莉梅1, 刘秀丽1, 李星云1, 白乌日力嘎1, 赵俪捷1, 韩云飞1, 刘璐1, 史培1, 乔健敏1, 傲特更脑日布2   

  1. 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    2.内蒙古牧名食品有限责任公司,内蒙古 鄂尔多斯 017399
  • 收稿日期:2024-10-16 发布日期:2025-01-26
  • 通讯作者: 乔健敏(1988—),女,副研究员,博士,主要研究方向为乳品生物技术与工程。
  • 作者简介:郑伟(1994—),女,工程师,硕士,主要研究方向为乳品生物技术与工程。
  • 基金资助:
    鄂尔多斯市科技计划项目(2022YY029); 内蒙古自治区科技计划项目(2020GG0093)

The Effect of Different Sterilization Conditions on the Microorganisms of Acid Whey Beverages

ZHENG Wei1, LI Zijian1, ZHANG Yuanyuan1, WANG Limei1, LIU Xiuli1, LI Xingyun1, BAI Wuriliga1, ZHAO Lijie1, HAN Yunfei1, LIU Lu1, SHI Pei1, QIAO Jianmin1, Aotegengnaoribu2   

  1. 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031,China;
    2. Inner Mongolia Muming Foodstuffs Limited Liability Company, Ordos 017399,China
  • Received:2024-10-16 Published:2025-01-26

摘要: [目的] 探索既能杀灭致病菌和腐败微生物,又能最大限度地保留酸乳清饮料中乳酸菌活菌数的杀菌条件,为酸乳清饮料的生产流程优化提供参考。[方法] 以自然发酵的酸乳清为原料,制备酸乳清饮料;设置1个对照组和3个杀菌处理组,分别为A~D组。A组为对照组,不对样品进行杀菌处理,B组进行65 ℃、30 min杀菌处理,C组进行75 ℃、1 min杀菌处理,D组进行75 ℃、3 min杀菌处理。经不同杀菌条件处理后,检测酸乳清饮料中菌落总数以及乳酸菌、大肠菌群、金黄色葡萄球菌、霉菌和酵母菌的数量。[结果] 所有酸乳清饮料样本中均未检出大肠菌群;75 ℃、1 min杀菌处理组(C组)不能杀灭所有金黄色葡萄球菌、霉菌和酵母菌,65 ℃、30 min和75 ℃、3 min杀菌处理组(B组和D组)均可以完全杀灭金黄色葡萄球菌、霉菌和酵母菌,但75 ℃、3 min杀菌处理组(D组)的乳酸菌存活数更多。[结论] 75 ℃杀菌3 min是3个处理组中最佳的杀菌条件,可以作为酸乳清饮料生产加工流程优化的参考。

关键词: 酸乳清饮料, 杀菌条件, 微生物, 乳酸菌

Abstract: [Objective] The aim of this study was to explore the sterilization conditions that can both eliminate pathogenic bacteria and spoilage microorganisms while maximally preserving the viable count of lactic acid bacteria, providing a theoretical reference for optimizing the production process of acid whey beverages. [Method] Acid whey was used to prepare acid whey beverages, and then one control group and three sterilization treatment groups, named A to D, were set up. Group A was the control group without sterilization, group B was sterilized at 65 ℃ for 30 min, group C was sterilized at 75 ℃ for 1 min, and group D was sterilized at 75 ℃ for 3 min. After different sterilization conditions, the total colony counts as well as the numbers of lactic acid bacteria, coliforms, staphylococcus aureus, moulds and yeasts were detected in the acid whey beverages. [Result] The coliform was not detected in the sample of acid whey beverage. The 75 ℃ for 1 min sterilization group (Group C) could not eliminate all staphylococcus aureus, moulds, and yeast. Both 65 ℃ for 30 min and 75 ℃ for 3 min sterilization treatment groups (Group B and D) could completely kill staphylococcus aureus, moulds and yeasts, but the 75 ℃ for 3min sterilization group (Group D) had a higher survival rate of lactic acid bacteria. [Conclusion] The 75 ℃ for 3 min sterilization treatment is the optimal sterilization condition among the three groups, which can serve as a reference for optimizing the production and processing process of acid whey beverages.

Key words: acid whey beverage, sterilization conditions, microorganism, lactic acid bacteria

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