畜牧与饲料科学 ›› 2018, Vol. 39 ›› Issue (1): 1-1.doi: 10.12160/j.issn.1672-5190.2018.01.001

• 基础科学 • 上一篇    下一篇

正交设计优化黄芪多糖提取工艺

李海欧[1];陈琪[1];林英华[1];雷雯朴[1];孙耀[1];段鹏飞[1];陈治宇[1];盛华[1];金蓉[2];赛那[1]   

  1. [1]内蒙古医科大学药学院,内蒙古呼和浩特010110;[2]内蒙古医科大学基础医学院,内蒙古呼和浩特010110
  • 出版日期:2018-01-20 发布日期:2018-01-20
  • 通讯作者: 李海欧
  • 作者简介:李海欧(1982-),女,助教,硕士,主要从事中蒙药新剂型的研究工作。;通讯作者:赛那(1986-),男,讲师,硕士,主要从事中蒙药新剂型的研究工作。
  • 基金资助:
    内蒙古医科大学大学生创新创业训练项目(2017101320017);内蒙古医科大学实验室开放基金项目(2016ZN16);内蒙古医科大学英才培育计划项目(2016YCPY030)

Optimization of Extraction Technology of Astragalus Polysaccharide by Orthogonal Design

LI Hai-ou1, CHEN Qi1, LIN Ying-hua1, LEI Wen-pu1, SUN Yao1, DUAN Peng-fei1, CHEN Zhi-yu1, SHENG Hua1, JIN Rong2, Saina1 ( 1 .College of Pharmacy, Inner Mongolia Medical University, Hohhot O10110, China ; 2. Basic Medical College, Inner Mongolia Medical University,Hohhot 010110,China)   

  • Online:2018-01-20 Published:2018-01-20

摘要: [目的]优化黄芪多糖的提取工艺。[方法]以料液比、提取时间、提取次数、浸泡时间为试验因素,各因素分别设计3个水平,采用L_9(3~4)正交设计,以黄芪多糖含量为考察指标,优选黄芪多糖的水回流提取工艺。[结果]最佳提取工艺条件为:料液比1∶12,提取时间1.0 h,浸泡时间1.0 h,提取次数3次。在最佳提取工艺条件下,黄芪多糖含量占原药材的6.4%,多糖含量占粗多糖的58.23%。[结论]优化后的提取工艺操作简便、快捷,重复性、稳定性良好。

Abstract: [objective]To optimize the extraction technology of Astragalus polysaccharide. [Methods]The solid to liquid ratio,extraction duration, extraction times, and immersion duration were chosen as the experimental factors in Astragalus polysaccharide extraction, and there were three levels for each of the factor. The water reflux extraction technology of Astragalus polysaccharide was optimized by L_9(3~4) orthogonal test using Astragalus polysaccharide content as assessing index. [Results]The optimum extraction conditions were as followed: solid to liquid ratio was 1∶12, extraction duration was 1.0 h, extraction repeated 3 times, and immersion duration was 1.0 h. Under these conditions, the obtained Astragalus polysaccharide content accounted for 6.4% and 58.23% of the raw medicinal materials and crude polysaccharide, respectively. [Conclusion]The optimized extraction technology of Astragalus polysaccharide is simple and quick to operate and has good repeatability and stabilization.

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