畜牧与饲料科学 ›› 2019, Vol. 40 ›› Issue (8): 104-106.doi: 10.12160/j.issn.1672-5190.2019.08.024

• 疾病防治 • 上一篇    下一篇

犬脓皮病病原菌的分离鉴定及耐药性分析

彭华, 李淑红, 聂佳伟, 郭泽婷   

  1. 湖南文理学院生命与环境科学学院,湖南 常德 415000
  • 收稿日期:2019-06-18 出版日期:2019-08-30 发布日期:2019-12-17
  • 通讯作者: 李淑红(1964—),女,教授,硕士,主要研究方向为动物营养与兽医学。
  • 作者简介:彭华(1996—),男,所学专业为动物科学。
  • 基金资助:
    湖南文理学院2018年大学生创新创业研究项目(ZC1830)

Isolation, Identification and Antimicrobial Resistance Profile of Pathogenic Bacteria of Canine Pyoderma

PENG Hua, LI Shu-hong, NIE Jia-wei, GUO Ze-ting   

  1. College of Life and Environmental Science,Hunan University of Arts and Sciences,Changde 415000,China
  • Received:2019-06-18 Online:2019-08-30 Published:2019-12-17

摘要: 为了研究犬脓皮病致病菌的种类及其对抗生素的敏感性,采用细菌培养、分离、革兰染色和生化试验等方法鉴别病原菌;采用Kirby-Bauer药物纸片琼脂扩散法对病原菌进行药敏试验。结果表明:病犬发病部位的病原菌为中间型葡萄球菌和金黄色葡萄球菌。中间型葡萄球菌对头孢拉啶和头孢氨苄敏感,敏感率为20%;对羧苄西林和头孢他啶中度敏感,中度敏感率为20%;对红霉素、卡那霉素、复方新诺明、哌拉西林、青霉素和苯唑西林产生耐药,耐药率为60%。金黄色葡萄球菌对头孢拉啶和头孢氨苄敏感,敏感率为20%;对羧苄西林和哌拉西林中度敏感,中度敏感率为20%;对红霉素、卡那霉素、复方新诺明、头孢他啶、青霉素和苯唑西林产生耐药,耐药率为60%。

关键词: 脓皮病, 分离鉴定, 耐药性分析,

Abstract: The objective of the present study was to determine the causative agents of canine pyoderma and to characterize their antimicrobial susceptibility. The strains were isolated from the clinical samples and were subsequently identified by Gram staining method and biochemical test. The antimicrobial susceptibility test of the isolates was carried out using Kirby-Bauer disc agar diffusion method. The results showed that isolates of Staphylococcus intermediaS. intermedia) and Staphylococcus aureusS. aureus) was recovered from the clinical samples collected from the lesion location of the dogs with pyoderma. Twenty percent of the obtained S. intermedia isolates were susceptible to cefradine and cefalexin; the intermediate rate of the S. intermedia isolates against carbenicillin and ceftazidime was 20%; the S. intermedia isolates were resistant to erythromycin, kanamycin, compound trimethoprim, piperacillin, penicillin and oxacillin, with resistance rate of 60%. Twenty percent of the obtained S. aureus isolates were susceptible to cefradine and cefalexin; the intermediate rate of the S. aureus isolates against carbenicillin and piperacillin was 20%; the S. aureus isolates were resistant to erythromycin, kanamycin, compound trimethoprim, ceftazidime, penicillin and oxacillin, with resistance rate of 60%.

Key words: pyoderma, isolation and identification, antimicrobial susceptibility test, canine

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