畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (6): 28-36.doi: 10.12160/j.issn.1672-5190.2025.06.005

• 草业科学 • 上一篇    下一篇

Na2CO3和硼酸对甜高粱种子发芽及幼苗生长的影响

任亚玲, 罗天, 韦梦迪   

  1. 贵州农业职业学院,贵州 贵阳 551400
  • 收稿日期:2025-09-30 出版日期:2025-11-30 发布日期:2026-01-26
  • 作者简介:任亚玲(1995—),女,讲师,硕士,主要研究方向为动物微生物与免疫。

Effects of Na2CO3 and Boric Acid on Seed Germination and Seedling Growth of Sweet Sorghum

REN Yaling, LUO Tian, WEI Mengdi   

  1. Guizhou Vocational College of Agriculture, Guiyang 551400, China
  • Received:2025-09-30 Online:2025-11-30 Published:2026-01-26

摘要: [目的] 明确Na2CO3和硼酸对甜高粱种子发芽及幼苗生长的影响。[方法] 以中科甜438和968两种饲用甜高粱种子为研究对象,以超纯水为对照组,设置Na2CO3、硼酸2个试验组,Na2CO3组设置10、20、40、80、120、200 mmol/L 6个浓度梯度,硼酸组设置1、2、5、10、15、25、50、70 mmol/L 8个浓度梯度。试验条件为25 ℃恒温、避光培养;记录每日发芽数,试验结束时测量胚根长、胚芽长、鲜重、干重等指标,计算发芽率、发芽势、发芽指数、活力指数、盐害率、幼苗含水量等指标。通过一元一次线性回归方程和指数回归方程拟合Na2CO3对甜高粱种子发芽、幼苗生长的影响,计算临界浓度;通过隶属函数法评估甜高粱的适宜硼酸浓度。[结果] Na2CO3浓度在40 、80、120、200 mmol/L时,438种子发芽率、发芽势、发芽指数均显著(P<0.05)低于对照组,浓度为20、40、80、120、200 mmol/L 时,438幼苗的胚根长、鲜重均显著(P<0.05)低于对照组;Na2CO3浓度为80、120、200 mmol/L 时,968种子发芽率、发芽势、发芽指数均显著(P<0.05)低于对照组,Na2CO3浓度为10、20、40、80、120、200 mmol/L时,968幼苗的胚芽长、胚根长均显著(P<0.05)低于对照组。一元一次方程拟合下,438种子的适宜盐碱浓度、半致死浓度、致死浓度依次为34.52、87.90、206.66 mmol/L,968种子依次为46.82、79.84、186.85 mmol/L。测试范围内的硼酸对438和968种子的发芽率无显著(P>0.05)影响,硼酸浓度为1 mmol/L时,438种子的胚芽长和胚根长、968种子的胚根长均显著(P<0.05)高于对照组;硼酸浓度为5、10 、15、25、50、70 mmol/L时,968种子的胚根长、胚芽长、鲜重均显著(P<0.05)低于对照组;硼酸浓度为10 、15、25、50、70 mmol/L时,438种子的胚根长、胚芽长、鲜重均显著(P<0.05)低于对照组。通过隶属函数法计算得出,438、968种子最适宜硼酸浓度为1 mmol/L。[结论] 968甜高粱种子对轻度盐碱环境适应性较好,438甜高粱种子对中重度盐碱环境耐受性较强;在测试硼酸浓度范围内,1 mmol/L硼酸对438、968甜高粱幼苗生长具有促进作用,可作为上述品种甜高粱幼苗生长的适宜硼酸施用浓度。

关键词: Na2CO3, 硼酸, 甜高粱, 发芽, 幼苗

Abstract: [Objective] This study aimed to clarify the effects of Na2CO3 and boric acid on seed germination and seedling growth of sweet sorghum. [Methods] Two forage sweet sorghum cultivars, Zhongke Tian 438 and 968, were used as experimental materials. Using ultrapure water as the control, two experimental groups were established, Na2CO3 was tested at 0, 10, 20, 40, 80, 120 and 200 mmol/L, and boric acid at 0, 1, 2, 5, 10, 15, 25, 50 and 70 mmol/L. Germination tests were conducted under constant temperature 25 ℃ and dark conditions, daily germination counts were recorded. At the end of the test, radicle length, plumule length, fresh weight, and dry weight were measured. Germination percentage, germination potential, germination index, vigour index, salt injury rate, and seedling water content were calculated. The effects of Na2CO3 on seed germination and seedling growth were fitted using linear regression and exponential regression equations to determine critical concentrations. The membership function method was applied to evaluate the suitable boric acid concentration for sweet sorghum. [Results] For cultivar 438, germination rate, germination potential, and germination index were significantly lower than those of the control at Na2CO3 concentrations of 40, 80, 120, and 200 mmol/L (P<0.05). Radicle length and fresh weight were significantly lower at concentrations of 20, 40, 80, 120, and 200 mmol/L (P<0.05). At Na2CO3 concentrations of 80, 120, and 200 mmol/L, the germination rate, germination potential, and germination index of cultivar 968 seeds were significantly lower than those of the control group (P<0.05); while at concentrations of 10, 20, 40, 80, 120, and 200 mmol/L, both plumule length and radicle length of cultivar 968 seedlings were significantly lower than control group (P<0.05). Based on linear regression fitting, the suitable saline alkali concentration, semi lethal concentration, and lethal concentration for cultivar 438 seeds were 34.52, 87.90, and 206.66 mmol/L, respectively; for cultivar 968 seeds, the corresponding values were 46.82, 79.84, and 186.85 mmol/L. Boric acid within the tested range had no significant effect on germination rate of either cultivar (P>0.05). At a boric acid concentration of 1 mmol/L, plumule and radicle lengths of cultivar 438 and radicle length of cultivar 968 were significantly higher than those of the control (P< 0.05). However, at boric acid concentrations of 5, 10, 15, 25, 50, and 70 mmol/L, radicle length, plumule length, and fresh weight of cultivar 968 were significantly lower than control group (P<0.05). Similarly, at boric acid concentrations of 10, 15, 25, 50, and 70 mmol/L, these parameters of cultivar 438 seeds were significantly lower than those of the control (P<0.05). The membership function analysis indicated that the optimal boric acid concentration for both cultivars was 1 mmol/L. [Conclusion] Cultivar 968 sweet sorghum seeds showed better adaptability to mild saline-alkali stress, while cultivar 438 exhibited stronger tolerance to moderate and severe saline-alkali conditions. Within the tested concentration range, 1 mmol/L boric acid promoted seedling growth of both sweet sorghum cultivars and can be recommended as a suitable concentration for seedling cultivation of these varieties.

Key words: Na2CO3, boric acid, sweet sorghum, germination, seedling

中图分类号: