Abstract: A strain BX-6 with good bacteriostatic activity was isolated from fresh chicken manure by routine microbiological method. Based on the means of colony morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence phylogeny analysis, the strain was identified and classified. And the antimicrobial characters of bacteriocin produced by BX-6 was detected by cylinder-plate method. The results showed that strain BX-6 was identified as Bacillus subtilis. Some antibacterial substance of H202 was produced in the fermentation process of BX-6. The bacteriocin had a good thermal stability,pH tolerance and it was sensitive to most proteases. And it had better antibacterial effects on Salmonella pullorum C79-13 ,chicken Escherichia coil O1 and Staphylococcus aureus C56011. So it had the potential to be used as feed additive of livestock and poultry.