Abstract: The interaction between DNA and protein is one important field of gene expression regulation in the post-genome era. There are many methods to study the interaction between DNA and protein at present, but chromatin immunoprecipitation assay （CHIP） is more suitable comparing with them. Besides, cloning technique and DNA chip technology could be combined to high-throughput screen the unknown DNA targets of known protein factors and study the distribution of trans-acting factors in whole genome so as to enhance the development and occurrence of DNA-protein interaction regulatory networks. The research progress of ChIP was introduced, and its prospect was prospected.