Abstract: The study was conducted to compare the performance of montmorillonite in absorbing AFB_1 and ZEN in vitro.Commercially available ELISA kits were used to assess the adsorption efficiency of montmorillonite for AFB_1 and ZEN under the condition of pH value 3.0 and 6.5, respectively. The results showed that under the condition of pH value 3.0, the adsorption rate and adsorption capacity of montmorillonite for AFB_1 were 88.00% and 440.00 μg/g, and those for ZEN were 7.50% and 37.50 μg/g; under the condition of pH value 6.5, the adsorption rate and adsorption capacity of montmorillonite for AFB_1 were 84.00% and 420.00 μg/g, and those for ZEN were 6.60% and 33.00 μg/g. It was demonstrated that montmorillonite performed well in absorbing of AFB_1 in vitro; however, it showed poor ability in absorbing ZEN.