Abstract: In order to verify the coincidence of near-infrared reflectance spectroscopy （NIRS） and conventional chemical assay （CCA）, the contents of moisture, crude protein, crude fat, crude ash, acid detergent fiber （ADF） and neutral detergent fiber （NDF） in 20 alfalfa samples from different sources were determined by NIRS and CCA. The results showed that the NIRS prediction model was more suitable for the determination of crude protein, crude fat, crude ash, ADF and NDF of alfalfa compared to CCA, but it was not suitable for the determination of moisture content. Accordingly, the existing NIRS prediction model needs to be adjusted and optimized.