数字PCR技术在动物疫病检测中的应用

doi:10.12160/j.issn.1672-5190.2021.06.021

Abstract

Abstract: In order to support the control and elimination plan in areas where animal epidemic diseases are prevalent, and to conduct effective screening in areas where animal epidemic diseases are not prevalent, more accurate and sensitive diagnostic methods are expected be used. Digital PCR assay can be used in absolute quantification and detection of trace amounts of nucleic acids. Compared with the traditional detection methods, it does not rely on reference substances or standard products, shows higher tolerance to inhibitors, and has higher sensitivity and accuracy. As a novel tool for quantitative analysis of nucleic acid, digital PCR assay has recently been employed in the detection of animal epidemic diseases. This article summarizes and analyzes the principles, applications, existing problems, and developmental trends of digital PCR assay, in hoping to provide references for its further applications in detection of animal epidemic diseases.

Key words: digital PCR assay, animal epidemic diseases, animal quarantine, detection

CLC Number:

S851.34

CHEN Lin-jun, CUI Qiang, YU Zhi-chao, LI Jun-yan, LUO Xiao-ping, WANG Hai-yan, YAN Han, LIU Dan, ZHAO Zhi-guo. Application of Digital PCR Assay in Detection of Animal Epidemic Diseases[J]. Animal Husbandry and Feed Science, 2021, 42(6): 124-128.

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Application of Digital PCR Assay in Detection of Animal Epidemic Diseases

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