新疆部分地区规模化猪场猪囊等孢球虫的套式PCR检测及感染情况分析

doi:10.12160/j.issn.1672-5190.2022.06.021

Abstract

Abstract:

[Objective] The present study aimed to understand the situation of Cystoisospora suis infection in large-scale pig farms in partial areas of Xinjiang, China. [Method] Primers targeting the SSU rDNA gene locus of Cystoisospora suis were designed. A total of 801 DNA samples extracted from fresh pig feces collected from 7 large-scale pig farms in Xinjiang were screened for the presence of target gene by using nested PCR assay. Cystoisospora suis infection rates in pigs of various farms and ages were statistically assessed. The obtained SSU rDNA sequences were bioinformatically compared and analyzed, and a phylogenetic tree was constructed. [Result] The general infection rate of Cystoisospora suis in the surveyed pig farms was 5.74% （46/801）, and the highest infection rate （14.00%, 14/100） was observed in the pig farm in Shaya County. Cystoisospora suis infection rates among various pig farms were statistically significantly （χ²=27.081, df=6, P<0.01） different. In unweaned piglets, weaned piglets, fattening pigs and sows, the Cystoisospora suis infection rates were 14.79% （25/169）, 5.78% （13/225）, 2.31% （4/173） and 1.71% （4/234）, respectively. Cystoisospora suis infection rates in pigs of various ages were statistically significantly （χ²=36.366，df=3，P<0.01） different. In sequence comparison and phylogenetic analysis, the SSU rDNA sequences （n=46） of Cystoisospora suis obtained in this study had 100% homology with the SSU rDNA sequence of a Cystoisospora suis isolate from Fengyang County, Anhui Province （GenBank accession number: KX808495）, and they were genetically categorized in the same subgroup. [Conclusion] Infections caused by Cystoisospora suis were common in large-scale pig farms in partial areas of Xinjiang, so detection and prevention should be strengthened.

Key words: Cystoisospora suis, nested PCR, detection, infection rate

CLC Number:

S858.282.723

PENG Xia, ZHAO Qian-ming, WANG Ling-yun, XIN Lu-yao, ZHAO Ai-yun, YU Fu-chang, QI Meng. Nested PCR-based Detection and Infection Situation Analysis of Cystoisospora suis in Large-scale Pig Farms in Partial Areas of Xinjiang，China[J]. Animal Husbandry and Feed Science, 2022, 43(6): 124-128.

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Nested PCR-based Detection and Infection Situation Analysis of Cystoisospora suis in Large-scale Pig Farms in Partial Areas of Xinjiang，China

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