Abstract:

[Objective] To investigate the effects of gonadotropin-inhibitory hormone （GnIH） on feed intake, body weight and blood glucose homeostasis in male mice. [Method] A total of 20 male mice of similar weights and days of age were randomly divided into a control group （n=10） which received an intraperitoneal injection of 100 μL physiological saline per time per mouse, and an experimental group （n=10） which received an intraperitoneal injection of 20 μg/100 μL GnIH with 100 μL per time per mouse. The mice received the intraperitoneal injection of physiological saline or GnIH twice a day for consecutive 21 days. The feed intake of the mice were observed and recorded, and their weights, fasting blood glucose, glucose tolerance and insulin tolerance were determined. In addition, quantitative PCR （qPCR） was used to assess the mRNA relative expression levels of insulin transcription factor gene （NeuroD1）, insulin gene （Ins）, glucagon gene （Gcg） and insulin transcription regulative factor gene （Pdx1） in pancreas of the mice. [Result] Compared with the control group, the average daily gain and average daily feed intake of the experimental group were extremely significantly （P<0.01） higher. The area under the curve （AUC） of fasting blood glucose test, the AUC of glucose tolerance test and the AUC of insulin tolerance test of the experimental group all increased extremely significantly （P<0.01). In the experimental group, significantly （P<0.05） higher mRNA relative expression level of Gcg gene in pancreas was observed, while the significantly （P<0.05） lower mRNA relative expression levels of Ins, Pdx1 and NeuroD1 genes were found. [Conclusion] Chronic intraperitoneal injection of GnIH caused increased feed intake and body weight as well as disordered blood glucose in male mice.

Key words: GnIH, mice, insulin, blood glucose, body weight