沙门菌感染对小鼠白细胞总数及其亚群动态变化的影响

doi:10.12160/j.issn.1672-5190.2020.06.003

Abstract

Abstract: The present study aimed to assess the effect of Salmonella infection on the dynamic changes of the total number of white blood cells and their subgroups in mice. This study adopted intraperitoneal injection to inoculate mice with Salmonella at a concentration of 6.5×10⁹ CFU/mL. The blood samples of mice were collected at 4, 6, 8, 12, 16 and 24 h, respectively, and detected by an automatic animal blood cell analyzer. The total number of white blood cells, the number of lymphocytes, the number of monocytes, and the number of granulocytes in the infected mice were recorded and analyzed for the dynamic changes of the total number of white blood cells and their subgroups within 24 h. The results showed that the total number of white blood cells and their subgroups all increased in the initial stage of infection and then decreased, and the total number of white blood cells and the number of granulocytes had the most significant changes, which were significantly （P<0.01） different from the blank control group. Mice Salmonella infection can stimulate the immune response of mice and affect the dynamic changes of the total number of white blood cells and their subgroups in the blood.

Key words: Salmonella, mice, total number of white blood cells, granulocytes, lymphocytes, monocytes

CLC Number:

SUN Shan-wen, ZHAO Tong, XIE Yi-meng, JIANG Peng-xuan, WANG Xin. Effect of Salmonella Infection on the Dynamic Changes of Total Number of White Blood Cells and Their Subgroups in Mice[J]. Animal Husbandry and Feed Science, 2020, 41(6): 13-16.

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References

[1] 李卓阳,郭荣显,孟闯,等.肠炎沙门菌与鸡蛋:污染机制、危害分析及生物防控[J].中国家禽,2018,40(17):40-44.
[2] 徐昊,孙海英,翟军军,等.大庆屠宰牛肉中沙门氏菌的分离鉴定及药敏试验[J].黑龙江八一农垦大学学报,2015,27(3):36-39.
[3] 张俊霞,冯永淼,李蓉,等.沙门菌疫苗的应用现状及研究前景[J].畜牧与饲料科学,2020,41(4):111-114.
[4] 谭烁,冯帆,陈极奎,等.一株山羊源肠炎沙门氏菌对小鼠的致病性研究[J].安徽农业科学,2018,46(13):90-94,143.
[5] 熊学丽,杨凡.血液感染诊断中C反应蛋白、降钙素原及白细胞计数的应用价值[J].医学综述,2016,22(8):1628-1630.
[6] HIDANAH S,SABDONINGRUM E K,WAHJUNI R S,et al.Effects of meniran (Phyllanthus niruri L.) administration on leukocyte profile of broiler chickens infected with Mycoplasma gallisepticum[J].Veterinary World, 2018, 11(6):834-839.
[7] 赵雪芹. 抗菌肽类似物JH-3抵御沙门氏菌感染的作用机制研究[D].新乡:河南科技学院,2018.
[8] OSVALDOVA A,STEPANOVA H,et al.FALDYNA M.Gene expression values of pattern-recognition receptors in porcine leukocytes and their response to Salmonella enterica serovar Typhimurium infection[J].Research in Veterinary Science, 2017, 114:31-35.
[9] 江俏,庞路勤,李伟征,等.白细胞计数、C反应蛋白及血清降钙素原联合检测诊断细菌性感染的效果评价[J].中国医学工程,2019,27(7):62-64.
[10] 孙伟,刘晓梅,周峰,等.大肠杆菌致小鼠急性腹膜炎模型的建立[J].齐齐哈尔医学院学报,2008(17):2049-2051.
[11] BOELEN A,BOORSMA J,KWAKKEL J,et al.Type 3 deiodinase is highly expressed in infiltrating neutrophil
[12] ic granulocytes in response to acute bacterial infection[J].Thyroid,2008, 18(10):1095-1103.
[13] 张林吉,任士飞,孙朋.中药复方对雏鸭感染沙门菌药效学试验[J].中兽医医药杂志,2018,37(3):5-8.
[14] KAISER P,REGOES R R,HARDT W D.Population dynamics analysis of ciprofloxacin-persistent S. typhimurium cells in a mouse model for Salmonella diarrhea[J].Methods in Molecular Biology,2016,1333:189-203.
[15] 朱波,营凡,李庆贺,等.不同异嗜性粒细胞与淋巴细胞比值雏鸡对鼠伤寒沙门菌免疫应答反应差异[J].中国家禽,2019,41(5):16-20.

Effect of Salmonella Infection on the Dynamic Changes of Total Number of White Blood Cells and Their Subgroups in Mice

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