吉林省动物源粪肠球菌分离鉴定及耐药性检测

doi:10.12160/j.issn.1672-5190.2025.04.017

Abstract

Abstract: [Objective] To investigate the antimicrobial resistance profiles of Enterococcus faecalis isolated from animal sources in Jilin Province. [Methods] A total of 400 samples were collected from large-scale layer chicken farms, pig farms, and dairy cow farms across eight prefecture-level cities and one autonomous prefecture in Jilin Province. Bacterial isolation and purification were performed, followed by identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry （MALDI-TOF MS） and susceptibility to 18 antibiotics was tested using the broth microdilution method. [Results] A total of 87 E. faecalis strains were isolated, with an overall isolation rate of 21.75% （87/400）. Specifically, 43 strains were isolated from chickens （isolation rate：30.71%, 43/140）, 33 from pigs （isolation rate：20.63%, 33/160）, and 11 from dairy cows （isolation rate： 11.00%, 11/100）. Chicken-derived E. faecalis exhibited resistance rates exceeding 80% to cefoxitin, clindamycin, ceftiofur, tiamulin, tilmicosin, tetracycline, erythromycin, and oxacillin. Pig-derived E. faecalis showed resistance rates above 80% to cefoxitin, clindamycin, ceftiofur, tiamulin, tetracycline, erythromycin, oxacillin, gentamicin, and tilmicosin. Dairy cow-derived E. faecalis displayed a 100% resistance rate to tilmicosin, while resistance to other Antimicrobial （except gentamicin） was below 10.00%. Overall, the multidrug resistance rate was 85.07%, with resistance to 9 drugs being the most prevalent, accounting for 26.87% of isolates. [Conclusion] The resistance rates of E. faecalis from layer and pig farms in Jilin Province are high, with severe multidrug resistance observed.

Key words: Jilin Province, animal sources, Enterococcus faecalis, isolation and identification, antimicrobialresistance detection

CLC Number:

S852.611

YU Hui, LI Jinlei, FANG Zhongyi, YANG Xixiang, WEI Qian, CHANG Jiang. Isolation, Identification, and Antimicrobial Resistance Detection of Enterococcus faecalis from Animal Sources in Jilin Province[J]. Animal Husbandry and Feed Science, 2025, 46(4): 122-128.

References

[1] TEDIM A P, RUIZ-GARBAJOSA P, CORANDER J, et al.Population biology of intestinal Enterococcus isolates from hospitalized and nonhospitalized individuals in different age groups[J].Applied and Environmental Micro biology, 2015, 81(5):1820-1831.
[2] 王汉星. 饲粮添加枯草芽孢杆菌与粪肠球菌对断奶仔猪生长性能及肠道健康的影响[D].杨凌:西北农林科技大学,2020.
[3] 史自涛,姚焰础,江山,等.粪肠球菌替代抗生素对断奶仔猪生长性能、腹泻率、血液生化指标和免疫器官的影响[J].动物营养学报,2015,27(6):1832-1840.
[4] MATSUMOTO H, MIYAGAWA M, YIN Y Z, et al.Effects of organic acid, Enterococcus faecalis strain EC-12 and sugar cane extract in feed against enterotoxigenic Escherichia coli-induced diarrhea in pigs[J].AMB Express,2021,11(1):68.
[5] WANG K L, CAO G T, ZHANG H R, et al.Effects of Clostridium butyricum and Enterococcus faecalis on growth performance, immune function, intestinal morphology, volatile fatty acids, and intestinal flora in a piglet model[J].Food and Function,2019,10(12):7844-7854.
[6] SHEHATA A A, TARABEES R, BASIOUNI S, et al.Effect of a potential probiotic candidate Enterococcus faecalis-1 on growth performance, intestinal microbiota, and immune response of commercial broiler chickens[J].Probiotics and Antimicrobial Proteins,2020,12(2):451-460.
[7] JOAKIM LARSSON D G, FLACH C F. Antibiotic resistance in the environment[J].Nature Reviews Microbiology,2022,20(5):257-269.
[8] WALLACE J S, GARNER E, PRUDEN A, et al.Occurrence and transformation of veterinary antibiotics and antibiotic resistance genes in dairy manure treated by advanced anaerobic digestion and conventional treatment methods[J].Environmental Pollution,2018,236:764-772.
[9] 蒋逸凡. 湖南省猪、鸡源粪肠球菌及大肠杆菌耐药性与ERIC-PCR分型研究[D].长沙:湖南农业大学, 2020.
[10] 杨涛,陆怡,邓卫平,等.肠球菌耐药率与抗菌药物使用强度相关性研究[J].中国药业,2021,30(2):89-93.
[11] GRANDCLÉMENT C, SEYSSIECQ I, PIRAM A, et al. From the conventional biological wastewater treatment to hybrid processes, the evaluation of organic micropollutant removal:a review[J].Water Research,2017,111:297-317.
[12] 李金磊,董鹏,狄元冉,等.河南省部分地区猪和鸡源粪肠球菌的分离鉴定与耐药性分析[J].动物医学进展,2018,39(4):122-127.
[13] 徐琦琦,陈月月,李宏博,等.新疆伊犁昭苏不同动物源粪肠球菌耐药性及耐药基因检测[J].中国农业科技导报,2023,25(9):140-146.
[14] 高敬宇,钟忻桐,洪志杰,等.猪源粪肠球菌的分离鉴定及耐药性分析[J].吉林畜牧兽医,2024,45(9):28-30.
[15] 商军,顾欣,张文刚,等.猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析[J].中国抗生素杂志,2017,42(3):230-236.
[16] 王东. 新疆阿克苏地区猪场粪肠球菌耐药性调查及optrA和lsa(E)基因传播特点的研究[D].乌鲁木齐:新疆农业大学,2022.
[17] 王舒丰,轩慧勇,姚晓慧,等.自繁自养猪场中不同来源粪肠球菌与屎肠球菌的耐药性调查分析[J].中国动物传染病学报,2022,30(3):201-207.
[18] 章婧,蔡萍,贺奕卓,等.广东省养禽场肠球菌耐药性及毒力因子流行分布特征[J].畜牧兽医学报,2021,52(9):2650-2659.
[19] 刘雨. 泰安地区禽源粪肠球菌的分离鉴定及耐药性研究[D].泰安:山东农业大学,2021.
[20] 王瞳. 宁夏部分地区牛源肠球菌的分离鉴定与耐药性分析及相关基因的检测[D].银川:宁夏大学,2019.
[21] 陈万昭,轩慧勇,宋超慧,等.新疆昌吉地区某规模化猪场不同日龄猪源粪肠球菌耐药性及耐药基因分析[J].动物医学进展,2021,42(9):126-131.
[22] 张恩宝. 浙江省猪源粪肠球菌耐药性及optrA传播机制研究[D].杭州:浙江工商大学,2022.
[23] KUDO M, NOMURA T, YOMODA S, et al.Nosocomial infection caused by vancomycin-susceptible multidrug-resistant Enterococcus faecalis over a long period in a university hospital in Japan[J].Microbiology and Immunology,2014,58(11):607-614.

Isolation, Identification, and Antimicrobial Resistance Detection of Enterococcus faecalis from Animal Sources in Jilin Province

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