1株藏猪源解没食子酸链球菌的分离鉴定、生物学特性及致病性分析

doi:10.12160/j.issn.1672-5190.2026.02.015

Abstract

Abstract: [Objective] To identify the bacterial pathogen of Tibetan pigs with respiratory diseases in a Tibetan pig farm in Garze Prefecture, Sichuan Province, and to clarify its biological characteristics and pathogenicity. [Methods] The pathogenic bacteria in the diseased samples were isolated and cultured, followed by morphological observation, 16S rDNA molecular identification and whole-genome sequencing. Drug susceptibility testing, biofilm formation assay and mouse pathogenicity analysis were carried out to clarify its biological characteristics and pathogenicity. [Results] The isolated strain exhibited non-hemolytic activity on 5% sheep blood agar plates, with colonies approximately 1 mm in diameter, grayish-white, and smooth-edged; microscopic examination revealed Gram-positive cocci arranged in chains; PCR amplification was carried out using the universal primers for the bacterial 16S rDNA gene, and a target band of approximately 1,500 bp was obtained. Based on the phylogenetic tree constructed by 16S rDNA gene and the whole genome identification analysis, it was identified as Streptococcus gallolyticus.Drug susceptibility testing showed that the isolated strain was susceptible to ampicillin, penicillin, cephalexin, norfloxacin, vancomycin, imipenem, levofloxacin, chloramphenicol, ceftazidime and florfenicol, and resistant to gentamicin, polymyxin B, tetracycline and azithromycin. In addition, the isolated strain showed strong biofilm formation ability in vitro (+++) . The pathogenicity study in mice showed that the isolated strain was pathogenic to 6-week-old mice, and could cause depression, loss of appetite and acute death in mice after intraperitoneal injection. Necropsy revealed typical lesions such as liver congestion and lung congestion. Pathological tissue sections showed liver cell necrosis, lung inflammatory cell infiltration and cell damage. [Conclusion] A strain of S. gallolyticus from Tibetan pig was successfully isolated. The isolated strain has strong biofilm formation ability, resistance to certain antibiotics, and strong pathogenicity. The study results can provide a reference for the precise prevention and control of bacterial diseases in Tibetan pigs, as well as for public health safety.

Key words: Streptococcus gallolyticus, Tibetan pig, isolation and identification, pathogenicity

CLC Number:

S852.611

Jikenawei, LI Yueyi, JIANG Xiaotao, SHI Chuan, REN Haiyun, LI Xuejiao, LIU Jun, FENG Weidong, RAO Kaiqing. Isolation, Identification, Biological Characteristics, and Pathogenicity Analysis of a Streptococcus gallolyticus Strain Isolated from Tibetan Pig[J]. Animal Husbandry and Feed Science, 2026, 47(2): 116-128.

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Isolation, Identification, Biological Characteristics, and Pathogenicity Analysis of a Streptococcus gallolyticus Strain Isolated from Tibetan Pig

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