Abstract: The objective of the present study was to determine the causative agents of canine pyoderma and to characterize their antimicrobial susceptibility. The strains were isolated from the clinical samples and were subsequently identified by Gram staining method and biochemical test. The antimicrobial susceptibility test of the isolates was carried out using Kirby-Bauer disc agar diffusion method. The results showed that isolates of Staphylococcus intermedia （S. intermedia） and Staphylococcus aureus （S. aureus） was recovered from the clinical samples collected from the lesion location of the dogs with pyoderma. Twenty percent of the obtained S. intermedia isolates were susceptible to cefradine and cefalexin; the intermediate rate of the S. intermedia isolates against carbenicillin and ceftazidime was 20%; the S. intermedia isolates were resistant to erythromycin, kanamycin, compound trimethoprim, piperacillin, penicillin and oxacillin, with resistance rate of 60%. Twenty percent of the obtained S. aureus isolates were susceptible to cefradine and cefalexin; the intermediate rate of the S. aureus isolates against carbenicillin and piperacillin was 20%; the S. aureus isolates were resistant to erythromycin, kanamycin, compound trimethoprim, ceftazidime, penicillin and oxacillin, with resistance rate of 60%.

Key words: pyoderma, isolation and identification, antimicrobial susceptibility test, canine