北方农业学报 ›› 2020, Vol. 48 ›› Issue (5): 49-54.doi: 10.12190/j.issn.2096-1197.2020.05.09

• 种质资源·分子生物学 • 上一篇    下一篇

低谷蛋白水稻的淀粉颗粒扫描电镜观察及RVA谱特征研究

郭涛, 张焕霞, 薛芳, 王海凤, 姜艳芳, 房文文, 林香青, 张士永   

  1. 山东省水稻研究所/山东省水稻工程技术研究中心,山东 济南 250100
  • 收稿日期:2020-08-20 出版日期:2020-10-20 发布日期:2020-12-17
  • 通讯作者: 张士永(1968—),男,研究员,学士,主要从事优良食味粳稻新品种选育配套栽培技术的研究工作。
  • 作者简介:郭 涛(1982—),男,助理研究员,博士,主要从事水稻种质资源保护与创新利用的研究工作。
  • 基金资助:
    国家重点研发计划项目(2017YFD0100403); 山东省重点研发计划项目(2017CXGC0307); 山东省农业科学院农业科技创新工程项目(CXGC2016A02); 国家水稻产业技术体系项目(CARS-01-75); 山东省农业科技资金(园区产业提升工程)项目(2019YQ013); 山东种业集团联合育种项目

Scanning electron microscope observation and RVA spectrum characteristics of starch granules in a rice variety with low glutelin content

GUO Tao, ZHANG Huanxia, XUE Fang, WANG Haifeng, JIANG Yanfang, FANG Wenwen, LIN Xiangqing, ZHANG Shiyong   

  1. Shandong Rice Institute/Shandong Rice Engineering Technology Research Center,Ji′nan 250100,China
  • Received:2020-08-20 Online:2020-10-20 Published:2020-12-17

摘要: 【目的】探明稻米谷蛋白含量降低对淀粉颗粒结构及RVA(rapid viscosity analyzer)谱特征的影响。【方法】利用扫描电镜和快速黏度分析仪测定分析谷蛋白含量降低对稻米淀粉颗粒结构和淀粉RVA特征的影响。【结果】与背景亲本圣稻735相比,圣稻LG03的株高降低,穗粒数略微减少,总蛋白含量降低,其他农艺性状无显著差异。低谷蛋白含量新品系圣稻LG03的淀粉颗粒形态无显著变化,但大小均匀度降低;淀粉RVA分析表明,圣稻LG03的峰值黏度、热浆黏度、冷胶黏度、崩解值、糊化温度、到达峰值黏度时间均减少,而回复值和消减值增加。【结论】谷蛋白含量降低对稻米淀粉颗粒形态影响不大,但颗粒大小均匀度降低,导致其淀粉RVA特征值发生改变。

关键词: 低谷蛋白, 淀粉颗粒, 淀粉RVA谱

Abstract: 【Objective】To determine the effect of reduced glutenin content on starch grain structure and RVA(rapid viscosity analyzer)spectrum characteristics.【Methods】 Investigate the effect of reduced glutenin content on grain structure and starch RVA spectrum characteristics by the Scanning Electron Microscope(SEM) and viscosity analyzer.【Results】Compared with the background parent Shengdao 735,the plant height of Shengdao LG03 was reduced,the number of grains per panicle was slightly reduced,the total protein content was reduced,and there were no significant differences in other agronomic traits.Compared with Shengdao 735,new low-gluten content Shengdao LG03 had no significant change on the morphology of starch granules,but the size uniformity was reduced,and starch RVA curve showed that peak viscosity(PKV),hot paste viscosity(HPV),cool paste viscosity(CPV),breakdown viscosity(BDV),pasting temperature(PaT)and peak time(PeT)all had downward trend,while consistence viscosity(CSV)and setback viscosity(SBV)increased.【Conclusion】 The decrease of gluten content had little effect on the morphology of rice starch granules,but the uniformity of particle size was reduced,that may be the reason of the changes of starch RVA characteristic values.

Key words: Low glutelin protein, Starch granules, Starch RVA spectrum

中图分类号: 

  • S511