不同燕麦品种抗氧化系统对秆锈病菌侵染的响应

doi:10.12190/j.issn.2096-1197.2021.04.14

摘要/Abstract

摘要： 【目的】明确秆锈病菌侵染对不同燕麦品种抗氧化系统的影响。【方法】选取感病品种坝莜1号、蒙燕1号和抗病品种坝莜18号、白燕5号为试验材料,以蒸馏水为对照（CK）,分析接种秆锈病菌1、3、5、7、9 d后燕麦叶片活性氧含量及其抗氧化酶活性的变化。【结果】感、抗病燕麦品种接菌后,叶片活性氧变化不同,抗病燕麦品种叶片H₂O₂含量积累多于感病燕麦品种,感病燕麦品种·O₂^-含量上升幅度大于抗病燕麦品种。秆锈病菌侵染后燕麦叶片SOD、POD、PAL、CAT活性较CK有所上升。接菌后,感、抗病燕麦品种叶片SOD活性分别在3、9 d达到峰值,感病品种蒙燕1号较CK分别显著上升63.2%、47.5%（P<0.05）,坝莜1号较CK分别显著上升50.0%、73.8%（P<0.05）。接菌后7~9 d,感病品种坝莜1号、蒙燕1号叶片CAT活性较CK分别显著上升24.8%~30.7%和20.4%~30.0%（P<0.05）;接菌后5~9 d,抗病品种坝莜18号、白燕5号叶片CAT活性分别显著上升27.6%~55.8%和37.2%~48.3%（P<0.05）。【结论】接种秆锈病菌后,燕麦抗病品种通过快速产生活性氧、提高防御酶活性,较感病品种更早地产生防御反应,提高抗病性。

关键词: 燕麦, 秆锈病菌, 活性氧, 防御酶

Abstract: 【Objective】To investigate the effects of stem rust infection on antioxidant system of different oat varieties.【Methods】The susceptible varieties Bayou No. 1 and Mengyan No. 1 and the resistant varieties Bayou No.18 and Baiyan No. 5 were selected as the test materials,and distilled water as a control,to analyze the changes of reactive oxygen species content and antioxidant enzyme activities in different organs of oat after inoculation with stem rust bacteria for 1,3,5,7,9 d.【Results】The changes of reactive oxygen species in leaves of susceptible and resistant oat varieties were different after inoculation. H₂O₂ content in leaves of resistant oat varieties was more accumulated than that of susceptible oat varieties,and the increase of ·O₂^- content in susceptible oat varieties was greater than that of resistant oat varieties. The activities of SOD,POD,PAL and CAT in oat leaves after inoculation with stem rust bacteria were higher than those in CK. After inoculation,SOD activity in leaves of susceptible and resistant oat varieties reached peak values on day 3 and 9,respectively. Compared with CK,susceptible variety Mengyan No. 1 was significantly increased by 63.2% and 47.5%（P<0.05）,and Bayou No. 1 was significantly increased by 50.0% and 73.8%（P<0.05）,respectively. During 7 to 9 days after inoculation,CAT activity in leaves of Bayou No. 1 and Mengyan No. 1 was significantly increased by 24.8%-30.7% and 20.4%-30.0% compared with CK（P<0.05）,respectively.During 5 to 9 days after inoculation,CAT activity in leaves of Bayou No. 18 and Baiyan No. 5 was significantly increased by 27.6%-55.8% and 37.2%-48.3%（P<0.05）,respectively.【Conclusion】After inoculation with stem rust bacteria,resistant oat varieties produced reactive oxygen species quickly and increased the activity of defense enzymes,which resulted in earlier defense response and improved disease resistance than susceptible varieties.

Key words: Oat, Stem rust bacteria, Reactive oxygen species, Defense enzymes

中图分类号:

S513

韩宇婷, 陈晓晶, 徐忠山, 刘景辉, 薛国兴. 不同燕麦品种抗氧化系统对秆锈病菌侵染的响应[J]. 北方农业学报, 2021, 49(4): 98-104.

HAN Yuting, CHEN Xiaojing, XU Zhongshan, LIU Jinghui, XUE Guoxing. The response of antioxidant system of different oat varieties to infection of stem rust[J]. Journal of Northern Agriculture, 2021, 49(4): 98-104.

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