北方农业学报 ›› 2022, Vol. 50 ›› Issue (3): 17-26.doi: 10.12190/j.issn.2096-1197.2022.03.03

• 分子生物学·生理生化 • 上一篇    下一篇

氨基寡糖素对绿豆叶片防御酶活性和产量的影响

陈文晋1, 赵存虎1, 贺小勇1, 范雅芳1, 张自强2, 孔庆全1, 杨永兴3, 鲍吝智4, 张雄飞5, 谢淑娟6   

  1. 1.内蒙古自治区农牧业科学院 植物保护研究所,内蒙古 呼和浩特 010031;
    2.内蒙古自治区农牧业科学院 特色作物研究所,内蒙古 呼和浩特 010031;
    3.包头市土默特右旗苏波盖乡综合保障和技术推广中心,内蒙古 美岱桥 014111;
    4.通辽市科尔沁左翼后旗农牧技术推广中心,内蒙古 甘旗卡 028199;
    5.内蒙古托克托县农牧技术推广中心,内蒙古 双河 010200;
    6.通辽市开鲁县麦新镇综合保障和技术推广中心,内蒙古 麦新 028413
  • 收稿日期:2022-03-10 出版日期:2022-06-20 发布日期:2022-08-09
  • 通讯作者: 孔庆全(1964—),男,副研究员,学士,主要从事食用豆类的栽培育种研究工作。
  • 作者简介:陈文晋(1988—),女,助理研究员,硕士,主要从事食用豆类的栽培育种研究工作。
  • 基金资助:
    内蒙古自治区农牧业科学院青年创新基金项目(2018QNJJNO8); 国家现代农业产业技术体系资助项目(CARS08-Z06)

Effects of amino-oligosaccharin on leaf defense enzyme activity and yield of mung bean

CHEN Wenjin1, ZHAO Cunhu1, HE Xiaoyong1, FAN Yafang1, ZHANG Ziqiang2, KONG Qingquan1, YANG Yongxing3, BAO Linzhi4, ZHANG Xiongfei5, XIE Shujuan6   

  1. 1. Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    2. Institute of Special Crop,Inner Monlolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    3. Baotou Tumed Right Banner Subogai Town Comprehensive Security and Technology Promotion Center,Meidaiqiao 014111,China;
    4. Tongliao Horqin Zuoyi Rear Banner Agricultural and Animal Husbandry Technology Promotion Center,Ganqika 028199,China;
    5. Inner Mongolia Togtoh County Agricultural and Animal Husbandry Technology Promotion Center,Shuanghe 010200,China;
    6. Tongliao Kailu County Maixin Town Comprehensive Support and Technology Promotion Center,Maixin 028413,China
  • Received:2022-03-10 Online:2022-06-20 Published:2022-08-09

摘要: 【目的】揭示氨基寡糖素不同处理对绿豆生长的影响。【方法】采用三因素五水平正交试验设计,设浸种浓度(A)、喷施浓度(B)和喷施时期(C)3个因素,每个因素设5个水平,比较分析氨基寡糖素不同处理对绿豆叶片防御酶(SOD、POD、PPO)活性、SPAD值和产量的影响。【结果】浸种浓度(A)对绿豆叶片SOD活性、POD活性和SPAD值的影响高于其他2个因素处理,喷施浓度(B)对绿豆叶片PPO活性的影响略高于其他2个因素处理。每个因素的不同水平下,浸种浓度A3对应的绿豆产量最高,为2 050.83 kg/hm2;喷施浓度B4对应的绿豆产量最高,为1 989.17 kg/hm2;喷施时期C3对应的绿豆产量最高,为1 931.67 kg/hm2。【结论】氨基寡糖素不同处理对绿豆产量的影响顺序为浸种浓度>喷施浓度>喷施时期,产量最优处理组合为A3B4C3,即浸种浓度为0.4 g/100 mL、喷施浓度为1.8 g/L、喷施时期为盛花期喷施2次。

关键词: 氨基寡糖素, 绿豆, 正交试验, 防御酶, SPAD值, 产量

Abstract: 【Objective】To analyse the impact of various amino-oligosaccharide treatments on mung bean growth.【Methods】The effects of amino-oligosaccharides on the activities of the mung bean defense enzymes(SOD,POD,and PPO),as well as SPAD value and yield,were compared and analyzed using an orthogonal experiment with three factors and five levels,including seed soaking concentration(A),spraying concentration(B),and spraying period(C).【Results】In comparison to the other two treatments,the effect of seed soaking concentration(A)on SOD activity,POD activity,and SPAD value of mung bean leaves was higher. Spraying concentration(B)had a marginally higher impact than the other two treatments on the PPO activity of mung bean leaves. Under different levels of each factor,seed soaking concentration A3 had the highest yield of 2 050.83 kg/hm2,spraying concentration B4 had the highest yield of 1 989.17 kg/hm2 and spraying period C3 had the highest yield of 1 931.67 kg/hm2.【Conclusion】Among the diffrent treatments,seed soaking concentration had the highest effect on the yield of mung bean,followed by the spraying concentration and spraying period. The optimal treatment combination for yield was A3B4C3,with seed soaking concentration at 0.4 g/100 mL,spraying concentration at 1.8 g/L,and spraying twice in the full flowering stage.

Key words: Amino-oligosacchain, Mung bean, Orthogonal experiment, Defence enzyme, SPAD value, Yield

中图分类号: 

  • S522