不同改良措施对黄瓜连作土壤细菌群落结构组成和多样性的影响

doi:10.12190/j.issn.2096-1197.2022.02.04

北方农业学报 ›› 2022, Vol. 50 ›› Issue (2): 28-37.doi: 10.12190/j.issn.2096-1197.2022.02.04

不同改良措施对黄瓜连作土壤细菌群落结构组成和多样性的影响

姜伟¹, 薛国萍¹, 白红梅¹, 杜金伟¹, 朱春侠¹, 李杰², 宋庆成³, 季淑丽³, 王黎胜⁴

1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
2.赤峰市农牧科学研究院,内蒙古赤峰 024031;
3.宁城县农牧业局,内蒙古宁城 024200;
4.呼伦贝尔市农畜产品质量安全中心,内蒙古海拉尔 021000

收稿日期:2022-01-03 出版日期:2022-04-20 发布日期:2022-06-20
作者简介:姜伟（1974—）,女,研究员,博士,主要从事设施园艺栽培、温室土壤连作障碍修复与生理的研究工作。薛国萍（1990—）,女,助理研究员,硕士,主要从事无土栽培、温室土壤连作障碍修复与生理的研究工作。姜伟和薛国萍为同等贡献作者。
基金资助:
内蒙古自治区科技计划项目（2019GG345）; 内蒙古自治区科技成果转化项目（2019CG025）; 赤峰市“科技兴蒙”行动重点专项（231）

Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil

JIANG Wei¹, XUE Guoping¹, BAI Hongmei¹, DU Jinwei¹, ZHU Chunxia¹, LI Jie², SONG Qingcheng³, JI Shuli³, WANG Lisheng⁴

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Chifeng Academy of Agricultural and Animal Husbandry Sciences,Chifeng 024031,China;
3. Agriculture and Animal Husbandry Bureau of Ningcheng,Ningcheng 024200,China;
4. Hulun Buir Agricultural and Livestock Product Quality and Safety Center,Hailar 021000,China

Received:2022-01-03 Online:2022-04-20 Published:2022-06-20

摘要/Abstract

摘要： 【目的】探究不同改良措施对黄瓜连作土壤微生物群落结构组成和多样性的影响,筛选出适宜的土壤改良措施。【方法】以黄瓜连作12年的土壤为研究对象,设置秸秆还田、小麦填闲、添加抗重茬生物菌肥、石灰氮消毒土壤处理,采用Illumina MiSeq测序平台对土壤细菌进行16S rRNA高通量测序分析。【结果】与CK相比,4种处理均增加了黄瓜连作土壤细菌群落的丰富度指数（Chao1）和多样性指数（Simpson、Shannon-Wiener）,秸秆还田、添加抗重茬生物菌肥处理增加显著（P<0.05）。细菌门水平的细菌群落组成无改变,但秸秆还田、小麦填闲、添加抗重茬生物菌肥处理拟杆菌门（Bacteroidetes）相对丰度增加显著（P<0.05）,小麦填闲、石灰氮消毒土壤处理放线菌门（Actinobacteria）和芽单胞菌门（Gemmatimonadetes）的相对丰度增加显著（P<0.05）;添加抗重茬生物菌肥、石灰氮消毒土壤处理酸杆菌门（Acidobacteria）的相对丰度降低显著（P<0.05）。4种处理属水平的细菌群落,新增菌属热多孢菌属（Thermopolyspora）、热单孢菌属（Thermomonospora）,均显著降低优势菌属双歧杆菌属（Bifidobacterium）相对丰度（P<0.05）。小麦填闲处理优势菌属类固醇杆菌属（Steroidobacter）相对丰度增加显著（P<0.05）;秸秆还田、添加抗重茬生物菌肥处理优势菌属Chryseolinea、纤维弧菌属（Cellvibrio）、黄异杆菌属（Flavisolibacter）相对丰度增加显著（P<0.05）。【结论】4种改良措施均改变了黄瓜连作土壤细菌群落结构组成、丰富度和多样性,以添加抗重茬生物菌肥处理效果最优,秸秆还田次之。

关键词: 改良措施, 黄瓜, 连作, 土壤细菌群落结构, 土壤细菌多样性指数

Abstract: 【Objective】The impacts of various treatments on the structure and diversity of soil microbial communities on continuous cucumber cropping were investigated to select appropriate soil improvement measures.【Methods】The soil that had been continuously cropped cucumbers for 12 years was treated with straw returning,wheat filling,anti-continuous cropping biological fertilizer,and lime nitrogen disinfection. The Illumina MiSeq sequencing platform was used to conduct a 16S rRNA high-throughput sequencing of soil microorganisms.【Results】 Compared with the control,Chao1 index,Simpson index of the four treatments all increased,with straw returning and anti-continuous cropping biological fertilizer treatment increased significantly（P<0.05）. At the bacterial phylum level,different treatments had no effect on the community composition. However the relative abundance of Bacteroidetes increased significantly（P<0.05）in the treatments of straw returning,wheat filling,and anti-continuous cropping biological fertilizer. In the treatment of wheat filling and lime nitrogen disinfection,the relative abundance of Actinobacteria and Gemmatimonadetes increased significantly（P<0.05）. In the treatment of anti-continuous cropping biological fertilizer and lime nitrogen disinfection,the relative abundance of Acidobacteria reduced significantly（P<0.05）. At genus level in the four treatment,the relative abundance of dominant bacteria Bifidobacterium was significantly reduced（P<0.05）with the appearence Thermopolyspora and Thermomonospora. In wheat filling,the relative abundance of the dominant bacteria Steroidobacteria increased significantly（P<0.05）. In straw returning and anti-continuous cropping biological fertilizer,the relative abundance of dominant bacteria chryseolinea,cellvibrio and flavisobacter increased significantly（P<0.05）.【Conclusion】In continuous cucumber cultivation,the four improvement measures affected the composition,richness,and diversity of the soil bacterial community. The optimum treatment was adding anti-continuous cropping biological fertilizer,followed by straw returning.

Key words: Treatment measures, Cucumber, Continuous cropping, Soil bacterial community composition, Soil Simpson index

中图分类号:

S642.2

姜伟, 薛国萍, 白红梅, 杜金伟, 朱春侠, 李杰, 宋庆成, 季淑丽, 王黎胜. 不同改良措施对黄瓜连作土壤细菌群落结构组成和多样性的影响[J]. 北方农业学报, 2022, 50(2): 28-37.

JIANG Wei, XUE Guoping, BAI Hongmei, DU Jinwei, ZHU Chunxia, LI Jie, SONG Qingcheng, JI Shuli, WANG Lisheng. Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil[J]. Journal of Northern Agriculture, 2022, 50(2): 28-37.

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不同改良措施对黄瓜连作土壤细菌群落结构组成和多样性的影响

Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil

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