灰略红链霉菌抑菌和降解功能及其作用机制研究进展

doi:10.12190/j.issn.2096-1197.2025.05.08

摘要/Abstract

摘要： 灰略红链霉菌（Streptomyces griseorubens）属于链霉菌属（Streptomyces）,具有抑制植物病原菌的生长和繁殖、降解木质纤维素类生物质固体废弃物等多种生理功能,可在农业生产、食品卫生及环境保护等领域广泛应用。文章对灰略红链霉菌的生物学特性、抑菌和降解功能及其作用机制进行了梳理和总结,并对未来灰略红链霉菌的应用前景及研究方向进行了展望,旨在为农业生产领域更好地开发和利用灰略红链霉菌提供依据。

关键词: 灰略红链霉菌, 抑菌功能, 降解功能, 作用机制, 研究进展

Abstract: Streptomyces griseorubens,a species of the genus Streptomyces,exhibits multiple physiological functions,including inhibition of the growth and reproduction of plant pathogens and degradation of lignocellulosic biomass solid waste. It has broad application in agricultural production,food hygiene,environmental protection,and other fields. This review summarizes the biological characteristics,antimicrobial and degradation functions,and underlying mechanisms of S. griseorubens. Furthermore,the future application prospects and research directions of S. griseorubens are discussed,aiming to provide a basis for the enhanced development and utilization of S. griseorubens in agricultural production.

Key words: Streptomyces griseorubens, Antimicrobial function, Degradation function, Mechanisms, Research progress

中图分类号:

S432.2

韩超, 朱宇飞, 李欣欣, 彭之东, 杜磊, 康文钦. 灰略红链霉菌抑菌和降解功能及其作用机制研究进展[J]. 北方农业学报, 2025, 53(5): 78-87.

HAN Chao, ZHU Yufei, LI Xinxin, PENG Zhidong, DU Lei, KANG Wenqin. Research progress on the antimicrobial and degradation functions of Streptomyces griseorubens and their mechanisms[J]. Journal of Northern Agriculture, 2025, 53(5): 78-87.

参考文献

[1] 夏焕章,翟航.放线菌次级代谢产物研究进展[J].微生物学杂志,2023,43(4):1-9.
[2] VAN BERGEIJK D A,TERLOUW B R,MEDEMA M H,et al.Ecology and genomics of Actinobacteria:new concepts for natural product discovery[J].Nature Reviews Microbiology,2020,18(10):546-558.
[3] 董雷,韩嘉瑞,李帅,等.链霉菌最新研究进展[J].微生物学报,2023,63(5):1815-1832.
[4] 李帅,董雷,韩嘉瑞,等.新疆古尔班通古特沙漠链霉菌:从可培养资源多样性到抗菌天然产物的生物合成潜力探索[J].中国科学:生命科学,2024,54(9):1699-1718.
[5] 潘明慧,杨雪,杜国忠,等.合成生物学助力链霉菌天然产物农药创制与产业化[J].植物保护,2023,49(5):371-389.
[6] FENG H W,ZHI Y E,SUN Y J,et al.Draft genome sequence of a novel Streptomyces griseorubens strain,JSD-1,active in carbon and nitrogen recycling[J].Genome Announcements,2014,2(4):e00650.
[7] 苏燕. 产抗菌物质海洋放线菌的筛选、鉴定及活性物质的研究[D].秦皇岛:燕山大学,2017.
[8] FENG H W,ZHI Y E,SHI W W,et al.Isolation,identification and characterization of a straw degrading Streptomyces griseorubens JSD-1[J].African Journal of Microbiology Research,2013,7(22):2730-2735.
[9] PRASAD P,SINGH T,BEDI S.Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No.AB184139) isolated from Indian soil[J].Journal of King Saud University - Science,2013,25(3):245-250.
[10] 罗艳青. 灰色链霉菌JSD-1发酵工艺及代谢产物研究[D].上海:上海交通大学,2015.
[11] 朱本伟,李富超,秦松.海洋链霉菌M506的鉴定及代谢产物抑菌活性初步研究[J].海洋科学,2012,36(12):45-49.
[12] 梁光杰,车程川,巩志金,等.一株海洋链霉菌发酵条件的优化及其抑菌活性物质的研究[J].中国酿造,2018,37(12):101-105.
[13] SUDHEER N S,BIJU I F,BALASUBRAMANIAN C P,et al.Probiotic potential of a novel endophytic Streptomyces griseorubens CIBA-NS1 isolated from Salicornia sp.against Vibrio campbellii infection in shrimp[J].Microbial Pathogenesis,2024,191:106677.
[14] PADEROG M J V,SUAREZ A F L,SABIDO E M,et al.Anthracycline shunt metabolites from philippine marine sediment-derived Streptomyces destroy cell membrane integrity of multidrug-resistant Staphylococcus aureus[J].Frontiers in Microbiology,2020,11:743.
[15] HAMED A,ABDEL-RAZEK A S,FRESE M,et al.New oxaphenalene derivative from marine-derived Streptomyces griseorubens sp. ASMR4[J].Zeitschrift Für Naturforschung B,2017,72(1):53-62.
[16] 张艳茹,郎剑锋,郭富超,等.禾谷镰刀菌拮抗放线菌21-3的筛选及鉴定[J].河南科技学院学报(自然科学版),2022,50(1):16-21.
[17] 韩超,李欣欣,韩建荣.堆肥提取液中大丽轮枝菌拮抗放线菌的分离、鉴定[J].山西农业科学,2016,44(3):328-332.
[18] BAKA Z A,RASHAD Y M,GHONEEM K M,et al.Streptomyces griseorubens E44G:a potent antagonist isolated from soil in saudi arabia[J].Journal of Pure and Applied Microbiology,2014,8(S1):221-230.
[19] SUNARYANTO R,PRAMISANDI A,RUDIYONO,et al.Isolation and screening of actinomycetes producing antimicrobial substances from Pulau Seribu[J].IOP Conference Series:Earth and Environmental Science,2024,1377(1):012084.
[20] SURYAMINARSIH P,KUSRININGRUM,NI′MATUZAROH,et al.Antagonistic compatibility of Streptomyces griseorubens,Gliocladium virens,and Trichoderma harzianum againts Fusarium oxysporum cause of tomato wilt deseases[J].International Journal of Plant and Soil Science,2015,5(2):82-89.
[21] SURYAMINARSIH P,WIRANTHI A,WINDRIYANTI W,et al.Study of the application of mixed entomopathogen formula Streptomyces griseorubens and Trichoderma harzianum on Non-Pest insects diversity in rice crops[J].Tec Empresarial,2024,19(2):36-46.
[22] ATALLAH B M,HAROUN S A,EL-MOHSNAWY E.Antibacterial activity of two actinomycetes species isolated from black sand in North Egypt[J].South African Journal of Science,2023,119(11/12):1-8.
[23] 马赛买,李同源,马燕军,等.几丁质酶在农作物病虫害生物防治中的研究进展[J].生物技术通报,2023,39(10):29-40.
[24] MERIEM G,MAHMOUD K.Optimization of chitinase production by a new Streptomyces griseorubens C9 isolate using response surface methodology[J].Annals of Microbiology,2017,67(2):175-183.
[25] RASHAD Y M,AL-ASKAR A A,GHONEEM K M,et al.Chitinolytic Streptomyces griseorubens E44G enhances the biocontrol efficacy against Fusarium wilt disease of tomato[J].Phytoparasitica,2017,45(2):227-237.
[26] AL-ASKAR A A,RASHAD Y M,HAFEZ E E,et al.Characterization of alkaline protease produced by Streptomyces griseorubens E44G and its possibility for controlling Rhizoctonia root rot disease of corn[J].Biotechnology and Biotechnological Equipment,2015,29(3):457-462.
[27] HAFEZ E,RASHAD Y M,ABDULKHAIR W M,et al.Improving the chitinolytic activity of Streptomyces griseorubens e44g by mutagenesis[J].Journal of Microbiology,Biotechnology and Food Sciences,2019,8(5):1156-1160.
[28] AL HAMAD B M,AL RAISH S M,RAMADAN G A,et al.Effectiveness of augmentative biological control of Streptomyces griseorubens UAE2 depends on 1-aminocyclopropane-1-carboxylic acid deaminase activity against Neoscytalidium dimidiatum[J].Journal of Fungi,2021,7(11):885.
[29] FARAG A M,ELSILK S E,GHONAM E B,et al.L-glutaminase production from new halophilic marine Streptomyces griseorubens NAHE isolated from mangrove sediment,Red Sea,Egypt[J].Egyptian Journal of Aquatic Biology and Fisheries,2024,28(3):167-189.
[30] 周潇,张云,黄洪波,等.深海来源链霉菌Streptomyces griseorubens scsio ZY0206多酚蒽酮类代谢产物的研究[J].天然产物研究与开发,2013,25(1):7-11.
[31] 刘占文,张利莉.生存胁迫法分离那拉提土壤拮抗放线菌及活性产物[J].塔里木大学学报,2019,31(2):13-22.
[32] 刘占文,万传星,张利莉.复合维生素对灰略红链霉菌TRM11107生产拒霉素的影响[J].生物资源,2019,41(1):69-74.
[33] NGUYEN Q H,NGUYEN H V,VU T H,et al.Characterization of endophytic Streptomyces griseorubens MPT42 and assessment of antimicrobial synergistic interactions of its extract and essential oil from host plant Litsea cubeba[J].Antibiotics,2019,8(4):197.
[34] ABDULKHAIR W M,ALGHUTHAYMI M A.Production and characterization of semi-quinolone antibiotic produced by Streptomyces griseorubens[J].Petroleum and Chemical Industry International,2022,5(3):137-149.
[35] HEO C S,KANG J S,YANG J W,et al.Labdane-type diterpenoids from Streptomyces griseorubens and their antimicrobial and cytotoxic activities[J].International Journal of Molecular Sciences,2024,25(6):3311.
[36] YANG W Z,LIANG G J,SUN Y,et al.Bioactive secondary metabolites from marine Streptomyces griseorubens f8:isolation,identification and biological activity assay[J].Journal of Marine Science and Engineering,2021,9(9):978.
[37] BAYGAR T,UGUR A.Biosynthesis of silver nanoparticles by Streptomyces griseorubens isolated from soil and their antioxidant activity[J].IET Nanobiotechnology,2017,11(3):286-291.
[38] EL-NAGGAR M Y,RAMADAN W,EL-HAMAMSY R A.The application of mediated biosynthesized green silver nanoparticles by Streptomyces griseorubens in water treatment[J].Journal of Pure and Applied Microbiology,2017,11(2):685-694.
[39] ANJUM M S,KHALIQ S,ASHRAF N,et al.Bioactive streptomycetes:a powerful tool to synthesize diverse nanoparticles with multifarious properties[J].Journal of Basic Microbiology,2024,64(9):e2400129.
[40] 马英辉,李利军,卢美欢,等.木质素降解放线菌的鉴定及产酶特性研究[J].环境污染与防治,2018,40(8):902-906.
[41] 徐杰,许修宏.Streptomyces griseorubens C-5对堆肥木质纤维素降解及微生物群落代谢的影响[J].太阳能学报,2018,39(2):285-291.
[42] 王战,冷雨茜,郭世荣,等.木薯渣基质化发酵微生物分离及复合菌配制[J].农业工程学报,2020,36(21):266-271.
[43] CHI C P.灰略红链霉菌JSD-1对猪粪和秸秆混合堆肥效果的影响及其微生态效应研究[D].上海:上海交通大学,2021.
[44] TUYEN D T,PHUONG D T H,VAN THANG L.Assessment of the mineralization process efficiency to decompose waste of dry bio-toilet of bio-NTK microbial inoculants[J].Biology Bulletin,2023,50(10):2872-2880.
[45] SARITHA M,ARORA A,SINGH S,et al.Streptomyces griseorubens mediated delignification of paddy straw for improved enzymatic saccharification yields[J].Bioresource Technology,2013,135:12-17.
[46] CHEN L Z,TANG T,WANG Z,et al.A novel fungal and bacterial consortium promotes the degradation of rice straw:conditions optimization and degradation properties[J].International Biodeterioration and Biodegradation,2024,194:105875.
[47] MILAD S,SALEH S E,ABOULWAFA M M,et al.Screening and characterization of soil bacteria for lignin and textile dye effluent bioremediation and optimization using response surface methodology[J].Scientific Reports,2025,15(1):25788.
[48] 冯海玮,孙玉静,毛亮,等.接种灰略红链霉菌(Streptomyces griseorubens)对水稻秸秆堆肥及还田后土壤理化性质和微生物群落影响[J].上海交通大学学报(农业科学版),2015,33(4):25-32.
[49] BOUBEKRI K,SOUMARE A,MARDAD I,et al.The screening of potassium- and phosphate-solubilizing Actinobacteria and the assessment of their ability to promote wheat growth parameters[J].Microorganisms,2021,9(3):470.
[50] SOUMARE A,BOUBEKRI K,LYAMLOULI K,et al.Efficacy of phosphate solubilizing Actinobacteria to improve rock phosphate agronomic effectiveness and plant growth promotion[J].Rhizosphere,2021,17:100284.
[51] CHI C P,CHU S H,WANG B,et al.Dynamic bacterial assembly driven by Streptomyces griseorubens JSD-1 inoculants correspond to composting performance in swine manure and rice straw co-composting[J].Bioresource Technology,2020,313:123692.
[52] ALSHAMSI A A A,SHETEIWY M S,ABUQAMAR S F,et al.Enhancement of mangrove growth performance using fish emulsion and halotolerant plant growth-promoting Actinobacteria for sustainable management in the UAE[J].Marine Pollution Bulletin,2024,199:115916.
[53] ASLAM T,LU A,RANA M,et al.Properties of cellulolytic Streptomyces strains from saline-sodic soil and their effects on rice growth under saline conditions[J].Pakistan Journal of Botany,2024,56(3):1163-1170.
[54] XU J,YANG Q.Isolation and characterization of rice straw degrading Streptomyces griseorubens C-5[J].Biodegrada-tion,2010,21(1):107-116.
[55] 徐杰. 水稻秸秆降解放线菌的分离鉴定及其降解机理研究[D].哈尔滨:哈尔滨工业大学,2011.
[56] 张俊,许超,张宇,等.纤维素酶降解机理的研究进展[J].华南理工大学学报(自然科学版),2019,47(9):121-130.
[57] 于跃,张剑.纤维素酶降解纤维素机理的研究进展[J].化学通报,2016,79(2):118-122.
[58] ZHANG D,LUO Y Q,CHU S H,et al.Biological pretreatment of rice straw with Streptomyces griseorubens JSD-1 and its optimized production of cellulase and xylanase for improved enzymatic saccharification efficiency[J].Preparative Biochemistry and Biotechnology,2016,46(6):575-585.
[59] AANAL P,JAIMIN P,APOORVA V,et al.Isolation and characterization of cellulase-producing actinomycetes from agricultural soils of gujarat,India:potential for liquid lignocellulosic waste degradation[J].Vidya-a Journal of Gujarat University,2025,4(1):148-159.
[60] FENG H W,SUN Y J,ZHI Y E,et al.Lignocellulose degradation by the isolate of Streptomyces griseorubens JSD-1[J].Functional and Integrative Genomics,2015,15(2):163-173.
[61] FENG H W,ZHI Y E,SUN Y J,et al.Insight into a novel β-1,4-glucosidase from Streptomyces griseorubens JSD-1[J].Applied Biochemistry and Microbiology,2016,52(4):371-377.
[62] ZHANG D,LUO Y Q,CHU S H,et al.Enhancement of cellulase and xylanase production using pH-shift and dissolved oxygen control strategy with Streptomyces griseorubens JSD-1[J].Applied Biochemistry and Biotec-hnology,2016,178(2):338-352.
[63] DOBARA M I A,EL-SAYED A K A,EL-FALLAL A A,et al.Immobilization and characterization of Streptomyces griseorubens xylanase[J].Egyptian Journal of Botany,2013(3):485-493.
[64] 周宁,王智伟,曹平华,等.产酸性木聚糖酶链霉菌的鉴定、酶学特性及发酵工艺研究[J].中国饲料,2016(8):16-21.
[65] FENG H W,SUN Y J,ZHI Y E,et al.Expression and characterization of a novel endo-1,4-β-xylanase produced by Streptomyces griseorubens JSD-1 isolated from compost-treated soil[J].Annals of Microbiology,2015,65(3):1771-1779.
[66] EL-SAYED M H,GOMAA A E F,ATTA O M,et al.Characteristics and kinetics of thermophilic actinomycetes′amylase production on agro-wastes and its application for ethanol fermentation[J].World Journal of Microbiology and Biotechnology,2024,40(8):255.
[67] FENG J,WANG B,ZHANG D,et al.Streptomyces griseo-rubens JSD-1 promotes rice straw composting efficiency in industrial-scale fermenter:evaluation of change in physicochemical properties and microbial community[J].Bioresource Technology,2021,321:124465.