外源精胺对碱胁迫下燕麦光合特性的影响

doi:10.12190/j.issn.2096-1197.2022.03.02

摘要/Abstract

摘要： 【目的】探索碱胁迫对燕麦光合特性的影响及外源精胺缓解效应,进而为盐碱地作物栽培及抗性品种选育提供参考。【方法】以抗碱品种蒙燕1号和碱敏感品种太阳神为试验材料,设置碱胁迫+Hoagland营养液、碱胁迫+精胺1+Hoagland营养液、碱胁迫+精胺2+Hoagland营养液、Hoagland营养液（对照）4个处理,对不同处理燕麦光合指标进行分析。【结果】碱胁迫抑制燕麦的光合作用。碱胁迫处理下,两个燕麦品种叶片叶绿素含量、光合速率、气孔导度、蒸腾速率均低于对照,气孔限制值提高,其中蒙燕1号叶绿素减幅低于太阳神;碱胁迫导致光系统Ⅱ（PSⅡ）的光反应过程发生变化,叶绿素荧光参数Fo升高,Fv/Fm、ABS/CSm、TrO/CSm、EtO/CSm、PI_ABS呈降低趋势,蒙燕1号的Fv/Fm、PI_ABS减幅低于太阳神。与碱胁迫相比,外源精胺有助于叶绿素累积,促进光合速率、气孔导度、蒸腾速率升高,叶绿素荧光参数EtO/CSm、PI_ABS提高,Fo降低;与碱胁迫相比,0.01 mmol/L精胺处理下PI_ABS升高达23.96%~125.25%,0.01 mmol/L精胺处理下光合速率、叶绿素含量的增幅高于0.06 mmol/L精胺处理。【结论】外源精胺可降低碱胁迫对燕麦PSⅡ的损伤效应,增加蒸腾拉力,缓解碱胁迫对气孔的限制作用,促进CO₂向叶绿体的运输,进而增强光合作用,0.01 mmol/L为精胺施用较佳浓度。

关键词: 精胺, 碱胁迫, 燕麦, 光合特性

Abstract: 【Objective】To investigate the effect of alkali stress on oat′s ability to produce oxygen and the mitigating effect of exogenous spermine,in order to provide guidance for the crop cultivation and breeding of resistant varieties in saline alkali soil.【Methods】Using alkali resistant variety Mengyan No. 1 and alkali sensitive variety Taiyangshen as test materials,four treatments were set up,including alkali stress+Hoagland nutrient solution,alkali stress+spermine 1+Hoagland nutrient solution,alkali stress+spermine 2+Hoagland nutrient solution and Hoagland nutrient solution as control. The photosynthetic indicators of oat under different treatments were analyzed.【Results】Alkali stress inhibited the photosynthesis of oats. Under alkali stress,the leaves of the two varieties had lower chlorophyll content,photosynthetic rate,stomatal conductance and transpiration rate compared the control while the stomatal limit value increased. Of these,the chlorophyll reduction of Mengyan No. 1 was lower than that of Taiyangshen. Alkali stress led to changes in the light reaction process of photosystem Ⅱ（PSⅡ）,with chlorophyll fluorescence parameters Fo increasing,while Fv/Fm、ABS/CSm、TrO/CSm、EtO/CSm、PI_ABS decreasing,among which the decrease of Fv/Fm and PI_ABS of Mengyan No. 1 was lower than that of Taiyangshen. Compared with alkali stress,exogenous spermine contributed to the accumulation of chlorophyll,increased photosynthetic rate,stomatal conductance and transpiration rate,raised chlorophyll fluorescence parameters EtO/CSm and PI_ABS,and decreased Fo. Under 0.01 mmol/L spermine treatment,PI_ABS increased by 23.96% to 125.25% when compared with alkali stress. The increase of photosynthetic rate and chlorophyll content under 0.01 mmol/L spermine treatment was higher than that under 0.06 mmol/L spermine treatment.【Conclusion】Exogenous spermine reduced the damaging effect of alkali stress on oat PS Ⅱ,increased transpiration tension,alleviated the limiting effect of alkali stress on stomata,improved photosynthesis by promoting the transport of CO₂ to chloroplasts. 0.01 mmol/L spermine was the optimal concentration.

Key words: Spermine, Alkali stress, Oat, Photosynthetic characteristics

中图分类号:

S512.6

白健慧, 王育青. 外源精胺对碱胁迫下燕麦光合特性的影响[J]. 北方农业学报, 2022, 50(3): 9-16.

BAI Jianhui, WANG Yuqing. Effects of exogenous spermine on photosynthetic characteristics of oat under alkali stress[J]. Journal of Northern Agriculture, 2022, 50(3): 9-16.

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