Journal of Northern Agriculture ›› 2025, Vol. 53 ›› Issue (2): 48-56.doi: 10.12190/j.issn.2096-1197.2025.02.05

• Molecular biology·Physiology and biochemistry • Previous Articles     Next Articles

Screening and evaluation of drought resistance indicators for different soybean varieties(lines) at the flowering and podding stage

CAO Jinjun1, GUO Zheng2, LI Dan1, LI Xiaoxia1, JIN Kunpeng1, LI Wanxing1, LIU Yongzhong2   

  1. 1. Millet Research Institute,Shanxi Agricultural University,Changzhi 046000,China;
    2. College of Agriculture,Shanxi Agricultural University,Taigu 030800,China
  • Received:2024-11-06 Online:2025-04-20 Published:2025-09-08

Abstract: 【Objective】To screen drought resistance indicators for different soybean varieties(lines) in Shanxi Province and improve the drought-resistant soybean germplasm resources.【Methods】Through pot experiments,15 soybean varieties(lines) at the flowering and podding stage were subjected to drought stress. Drought resistance coefficients(X),drought resistance membership function values[B(X)],and drought resistance measurement values(D-values) were determined and calculated for various agronomic traits[plant height(PH),stem diameter(SD)],photosynthetic parameters[stomatal conductance(Gs),net photosynthetic rate(Pn),relative chlorophyll content(SPAD)],and physiological and biochemical indicators[proline(Pro) content,soluble protein(Sp) content,malondialdehyde(MDA) content]. Principal component analysis and regression analysis were employed to comprehensively evaluate their drought resistance. Finally,a field validation experiment was conducted to calculate the drought resistance index(DHI).【Results】Drought stress significantly affected soybean agronomic traits,photosynthetic parameters,and physiological and biochemical indicators. The absolute mean values of drought resistance coefficients for individual indicators across different soybean varieties(lines),from highest to lowest,were:Pro content>MDA content>Sp content>SPAD>SD>PH>Pn>Gs. Principal component analysis revealed that the first three principal components contributed 84.321% of the total variance,representing the majority of the data information. The D-values for different soybean varieties(lines) were,in descending order:Y13>Y4>Y10>Y6>Y9>Y15>Y3>Y11>Y14>Y12>Y1>Y2 =Y7>Y8>Y5.The regression equation was:D-value=-3.459+0.756PH+1.299SD+0.858SPAD+0.382Pro content+0.693Sp content+0.397Pn.DHI results indicated that Y13 exhibited the strongest drought resistance,while Y5 showed the weakest.【Conclusion】Chang 1807(Y13) demonstrates the best drought resistance and can serve as a potential candidate for drought-tolerant varieties in Shanxi Province. Plant height,stem diameter,relative chlorophyll content,proline content,soluble protein content,and net photosynthetic rate exhibit significant statistical correlations with the drought resistance measurement value(D-value),thus serving as effective drought resistance indicators for different soybean varieties(lines) at the flowering and podding stage.

Key words: Soybean, Flowering and podding stage, Drought stress, Drought resistance indicators, Comprehensive evaluation

CLC Number: 

  • S565.1