畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (10): 1-1.doi: 10.12160/j.issn.1672-5190.2009.10.001

• 基础科学 •    下一篇

RNA干扰文库研究进展及其应用

武昱孜 张旭 张映   

  1. 山西农业大学动物科技学院,山西太谷030801
  • 出版日期:2009-10-20 发布日期:2009-10-20
  • 通讯作者: 武昱孜
  • 作者简介:武昱孜(1984-),女,硕士,主要研究方向为生物化学与分子生物学。 通讯作者:张映(1954-),女,教授,硕士生导师,主要研究方向为动物生物化学及分子免疫学,E-mail:dkyzy@126.com

Advance on RNA Interference Library and Its Application

WU Yu-zi, ZHANG Xu, ZHANG Ying (College of Animal Science and Technology, Shanxi Agriculture University, Taigu 030801, China)   

  • Online:2009-10-20 Published:2009-10-20

摘要: RNA干扰是一种在进化上保守的抵御转基因或外来病毒侵犯的防御机制,存在于许多不同种属的生物体中,在生物体细胞之间进行传递,具有抵抗病毒入侵和维持基因组稳定的作用。RNA干扰文库是以RNA干扰技术为基础建立的一种简单、高效、大规模、高通量的功能基因组学研究的工具,应用于大规模基因功能的筛选,在基因功能研究、发现新的药物靶基因和疾病相关基因等方面具有广阔的应用前景。

Abstract: RNA interference is an evolutionarily conserved defense mechanisms to resist transgenic genes and alien virus, it lies in many different species of organisms, and can transfer between organisms cells with the effect of resisting virus invasion and maintaining genome stahility. RNA interference library is based on RNA interference technology with the feature of the simplicity, efficiency, large- scale and high throughput, can be used in large scale screening of gene function and phenotype screening. Research and application of RNA interference as an extremely important theoretical and practical significance of the medical biology will have a profound impact on development.

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