红豆草缩合单宁降解菌的分离筛选及降解条件优化

doi:10.12160/j.issn.1672-5190.2025.01.004

畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (1): 25-31.doi: 10.12160/j.issn.1672-5190.2025.01.004

红豆草缩合单宁降解菌的分离筛选及降解条件优化

毋美茹¹, 李雅宁², 佟臻昊², 赵欣¹, 裴乐³, 李锋³, 段艳¹, 苏亚², 刘秀丽³

1.内蒙古农业大学,内蒙古呼和浩特 010018;
2.内蒙古自治区农畜产品质量安全中心,内蒙古呼和浩特 010010;
3.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031

收稿日期:2024-12-05 出版日期:2025-01-30 发布日期:2025-04-03
通讯作者: 刘秀丽（1978—）,女,研究员,博士,主要研究方向为新兽药及生物制品开发。
作者简介:毋美茹（2002—）,女,所学专业为食品质量与安全。
基金资助:
内蒙古自治区自然科学基金项目“红豆草缩合单宁降解菌的筛选及降解研究”（2021LHMS03016）

Isolation, Screening, and Optimization of Degradation Conditions for Condensed-Tannin-Degrading Bacteria from Onobrychis viciifolia

WU Meiru¹, LI Yaning², TONG Zhenhao², ZHAO Xin¹, PEI Le², LI Feng³, DUAN Yan¹, SU Ya², LIU Xiuli³

1. Inner Mongolia Agricultural University,Hohhot 010018, China;
2. Agricultural and Livestock Product Quality and Safety Center of Inner Mongolia Autonomous Region,Hohhot 010010, China;
3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031, China

Received:2024-12-05 Online:2025-01-30 Published:2025-04-03

摘要/Abstract

摘要： [目的]分离、筛选具有降解红豆草缩合单宁能力的乳酸菌,并优化其降解条件。[方法]将采自内蒙古和林格尔县草牧业园区实验田的红豆草自然发酵后,分离、筛选符合条件的乳酸菌;通过细菌培养基初筛和单宁降解率复筛,选择能较好降解红豆草缩合单宁的乳酸菌,并利用16S rDNA序列测定法对其进行鉴定。进一步通过探究不同接种量、培养时间及培养温度对该菌株红豆草缩合单宁降解率的影响,选择最优降解条件。[结果]红豆草发酵结束后筛选出33株符合条件的乳酸菌,经初筛及复筛后得到1株对红豆草缩合单宁有较好降解能力的乳酸菌YHD2-6,经鉴定为植物乳杆菌（Lactobacillus plantarum）。YHD2-6的最佳培养条件为接种量10%、培养时间96 h、培养温度37 ℃,此条件下红豆草缩合单宁的降解率可达到76.73%。[结论]本研究筛选得到的1株植物乳杆菌,对红豆草缩合单宁降解能力较强,不仅有助于提高含单宁饲料的营养价值,还在精细化学品领域具有潜在的应用价值。

关键词: 缩合单宁, 红豆草, 乳酸菌, 降解率, 条件优化

Abstract: [Objective] To isolate and screen lactic acid bacteria capable of degrading condensed tannins from Onobrychis viciifolia （sainfoin） and to optimize their degradation conditions. [Methods] Naturally fermented Onobrychis viciifolia samples,collected from experimental fields in the grassland and animal husbandry park of the Horinger County,Inner Mongolia, were used to isolate and screen lactic acid bacteria that met the specified criteria. Initial screening was conducted using bacterial culture media,followed by secondary screening based on tannin degradation rates to select strains with superior degradation capabilities for condensed tannins from Onobrychis viciifolia. The selected strain was identified using 16S rDNA sequence analysis. Furthermore,the effects of varying inoculum amounts,culture time,and culture temperature on the degradation rate of condensed tannins from Onobrychis viciifolia were investigated to determine the optimal degradation conditions. [Results] Following fermentation of Onobrychis viciifolia,33 strains of lactic acid bacteria meeting the criteria were isolated. After initial and secondary screening, one strain YHD2-6,exhibited strong condensed tannin degradation ability and was identified as Lactobacillus plantarum. The optimal culture conditions for YHD2-6 were determined to be an inoculum amount of 10%,a culture time of 96 h,and a culture temperature of 37 ℃,under which the degradation rate of condensed tannins reached 76.73%. [Conclusion] This study successfully screened a strain of Lactobacillus plantarum with a robust ability to degrade condensed tannins from Onobrychis viciifolia. This strain not only enhances the nutritional value of tannin-containing feeds but also holds potential application value in the field of fine chemicals.

Key words: condensed tannin, Onobrychis viciifolia, lactic acid bacteria, degradation rate, condition optimization

中图分类号:

毋美茹, 李雅宁, 佟臻昊, 赵欣, 裴乐, 李锋, 段艳, 苏亚, 刘秀丽. 红豆草缩合单宁降解菌的分离筛选及降解条件优化[J]. 畜牧与饲料科学, 2025, 46(1): 25-31.

WU Meiru, LI Yaning, TONG Zhenhao, ZHAO Xin, PEI Le, LI Feng, DUAN Yan, SU Ya, LIU Xiuli. Isolation, Screening, and Optimization of Degradation Conditions for Condensed-Tannin-Degrading Bacteria from Onobrychis viciifolia[J]. Animal Husbandry and Feed Science, 2025, 46(1): 25-31.

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红豆草缩合单宁降解菌的分离筛选及降解条件优化

Isolation, Screening, and Optimization of Degradation Conditions for Condensed-Tannin-Degrading Bacteria from Onobrychis viciifolia

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