骨碎补总黄酮对骨代谢的影响及作用机制

doi:10.12160/j.issn.1672-5190.2021.04.020

摘要/Abstract

摘要： 骨组织维持平衡的关键在于骨代谢的动态平衡。一旦骨代谢失衡,就会导致骨损伤甚至骨骼疾病。中药骨碎补含有主要活性成分——骨碎补总黄酮,该活性物质对骨质疏松、骨缺损、骨折和骨关节炎等骨骼疾病有治疗作用。对国内外相关文献进行归纳总结,从骨组织、血管、骨细胞以及调控机制四个方面综述骨碎补总黄酮对骨代谢的影响,为后续研究提供理论基础。

关键词: 骨碎补总黄酮, 信号通路, 骨代谢, 作用机制, 中药

Abstract: The key to maintain the balance of bone tissue is the dynamic balance of bone metabolism. The imbalance of bone metabolism results in bone damages and even bone diseases. Total flavonoids extracted from Rhizoma Drynariae, a traditional Chinese medicine, are active ingredients that have therapeutic effects on bone diseases such as osteoporosis, bone defect, bone fracture and osteoarthritis. Based on the summary of relevant literatures in China and abroad, this paper reviews the effects of total flavonoids from Rhizoma Drynariae on bone metabolism from the aspects of bone tissue, blood vessels, bone cells and related regulatory mechanisms, in hoping to provide theoretical basis for further researches.

Key words: Rhizoma Drynariae, signaling pathways, bone metabolism, action mechanisms, traditional Chinese medicine

中图分类号:

R285

张朝栋, 曹芹芹, 徐婷婷, 林露茜, 岳珂, 郑晶晶, 黄淑成. 骨碎补总黄酮对骨代谢的影响及作用机制[J]. 畜牧与饲料科学, 2021, 42(4): 118-123.

ZHANG Chao-dong, CAO Qin-qin, XU Ting-ting, LIN Lu-xi, YUE Ke, ZHENG Jing-jing, HUANG Shu-cheng. Effects of Total Flavonoids from Rhizoma Drynariae on Bone Metabolism and Action Mechanisms[J]. Animal Husbandry and Feed Science, 2021, 42(4): 118-123.

参考文献

[1] 南京中医药大学.中药大辞典[M].2版.上海:上海科技出版社,2006:1998.
[2] 李晋玉,赵学千,孙旗,等.骨碎补总黄酮的实验及临床研究概况[J].中国骨质疏松杂志,2018,24(10):1357-1364.
[3] 陈海啸,梁军波,伍海昭.骨碎补总黄酮对去卵巢大鼠腰椎松质骨骨形态计量学参数的影响[J].中医正骨,2011,23(4):18-20.
[4] 常安瑾,钱莹,陈晓农,等.维持性血液透析患者不同骨转换类型下松质骨微观结构的研究[J].中国血液净化,2017,16(1):48-53.
[5] 杨瑾廷,韩向龙.骨组织血管系统中血管形成与骨形成的偶联效应[J].中国组织工程研究,2017,21(36):(23): 5855-5861.
[6] ZHU S P,BENNETT S,KUEK V,et al.Endothelial cells produce angiocrine factors to regulate bone and cartilage via versatile mechanisms[J].Theranostics,2020,10(13):5957-5965.
[7] 上官文姬,李展春,程光齐,等.骨碎补总黄酮对血管内皮细胞功能和去卵巢大鼠血管形成的影响[J].中国中医骨伤科杂志,2017,25(1):5-8,13.
[8] NAN S,ZHAO Z H,MA X L,et al.Naringin promotes fracture healing through stimulation of angiogenesis by regulating the VEGF/VEGFR-2 signaling pathway in osteoporotic rats[J].Chemico-Biological Interactions,2017,261:11-17.
[9] 上官文姬,李鹤,汤璐敏.骨碎补总黄酮对大鼠成骨细胞VEGF和FGF-2表达的影响[J].辽宁中医药大学学报,2014,16(8):38-41.
[10] 童安莉,陈璐璐,丁桂芝.成骨细胞骨形成机制研究进展[J].中国骨质疏松杂志,1999(3):3-5.
[11] 盛华刚. 骨碎补对成骨细胞和破骨细胞作用的研究进展[J].化工时刊,2013,27(5):38-40.
[12] 张军,李浩鹏,杨平林,等.骨碎补总黄酮含药血清对成骨细胞增殖、分化、周期及凋亡的影响[J].中药材,2009,32(7):1090-1093.
[13] 黎小坚,HAROLD M F,朱绍舜,等.基础骨生物学新观[J].中国骨质疏松杂志,2001(2):59-81.
[14] LI F B,SUN X B,MA J X,et al.Naringin prevents ovariectomy-induced osteoporosis and promotes osteoclasts apoptosis through the mitochondria-mediated apoptosis pathway[J].Biochemical and Biophysical Research Communications,2014,452(3):629-635.
[15] 李思维,王静,陈林,等.维生素D与骨关节炎[J].中华关节外科杂志(电子版),2018,12(1):96-101.
[16] 李冬霞,陈颖颖,罗静,等.骨碎补总黄酮干预骨关节炎机制的研究进展[J].风湿病与关节炎,2019,8(3):66-69.
[17] 刘乐平,刘慧萍,谢瑾如,等.壮骨止痛方有效成分关联分析[J].中草药,2018,49(6):1451-1460.
[18] HANG K,YE C Y,XU J X,et al.Apelin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly through Wnt/β-catenin signaling pathway[J].Stem Cell Research and Therapy,2019,10(1):189.
[19] MURALIDHAR S,FILIA A,NSENGIMANA J,et al.Vitamin D-VDR signaling inhibits Wnt/β-Catenin-mediated melanoma progression and promotes antitumor immunity[J].Cancer Research,2019,79(23):5986-5998.
[20] LI Q Y,LAI Q H,HE C C,et al.RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer[J].Journal of Experimental and Clinical Cancer Research,2019,38(1):334.
[21] PING Z C,HU X Y,WANG L L,et al.Melatonin attenuates titanium particle-induced osteolysis via activation of Wnt/β-catenin signaling pathway[J].Acta Biomaterialia,2017,51:513-525.
[22] QI H H,BAO J,ZHANG Q,et al.Wnt/β-catenin signaling plays an important role in the protective effects of FDP-Sr against oxidative stress induced apoptosis in MC3T3-E1 cell[J].Bioorganic and Medicinal Chemistry Letters,2016,26(19):4720-4723.
[23] 胡中青,汤样华,曾林如,等.右归丸治疗骨质疏松性骨缺损的机制研究[J].中国中医骨伤科杂志,2018, 26(7):11-15,20.
[24] TOSHIHISA K.Regulation of bone development and extracellular matrix protein genes by RUNX2[J].Cell and Tissue Research,2010,339(1):189-195.
[25] 李晋玉,俞兴,姜俊杰,等.骨碎补总黄酮联合纳米骨材料促进MC3T3-E1细胞的增殖分化[J].中国组织工程研究,2020,24(7):1030-1036.
[26] 陈世海,谢兴文,李建国,等.Wnt/β-catenin信号通路在补肾中药靶向治疗骨质疏松症中应用的研究进展[J].中国骨质疏松杂志, 2019, 25(4):559-563.
[27] LV J W,SUN X L,MA J X,et al.Involvement of periostin-sclerostin-Wnt/β-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin[J].Biochemical and Biophysical Research Communications,2015,468(4):587-593.
[28] ATSUHIKO H,IKUO Y,WAKEYAMA H,et al.Negative regulation of osteoclastogenesis by ectodomain shedding of receptor activator of NF-kappaB ligand[J].The Journal of Biological Chemistry,2006,281(48):36846-36855.
[29] LI J,SUN Z Y,LIN Y,et al.Syndecan 4 contributes to osteoclast differentiation induced by RANKL through enhancing autophagy[J]. International Journal of Immunopharmacology, 2021,91:107275.
[30] MOHAMMAD T,MOHAMMED H M,AMIN N,et al.RANKL/RANK/OPG pathway: a mechanism involved in exercise-induced bone remodeling[J].BioMed Research International,2020,2020:6910312.
[31] 曾辉,赵许兵,唐成芳,等.骨碎补总黄酮对去卵巢大鼠下颌骨结构、血清E₂及OPG/RANKL的影响[J].西安交通大学学报(医学版),2019,40(2):328-332.
[32] 肖亚平,曾杰,焦琳娜,等.补肾中药对骨质疏松症的治疗及其信号通路调节作用的研究进展[J].中国中药杂志,2018,43(1):21-30.
[33] 施彦龙,李应福,谢兴文,等.BMP-Smads、OPG/RANK/RANKL信号通路与骨质疏松关系的研究进展[J].中国骨质疏松杂志,2020,26(4):600-604.
[34] 庞新岗,李永刚,包倪荣,等.骨代谢主要信号通路及信号分子的研究进展[J].基础医学与临床,2018,38(12):1799-1803.
[35] 王庆丰,王孝辉,张彩丽,等.骨碎补总黄酮促进大鼠诱导膜中VEGF、BMP-2表达的实验研究[J].药物评价研究,2018,41(7):1220-1223.
[36] 姜自伟,曾景奇,李悦,等.骨碎补总黄酮对大鼠胫骨牵张末期BMP-2与Smad-1表达的影响[J].辽宁中医杂志,2018,45(1):166-168,227.
[37] 沈智,曾景奇,李益亮,等.骨碎补总黄酮对悬尾实验性骨质疏松模型大鼠骨密度及BMP-2 mRNA的影响[J].中医药导报,2019,25(21):33-36.
[38] WU J B, FONG Y C, TSAI H Y, et al.Naringin-induced bone morphogenetic protein-2 expression via PI3K,Akt,c-Fos/c-Jun and AP-1 pathway in osteoblasts[J].European Journal of Pharmacology,2008,588(2/3):333-341.
[39] 丁祎,崔宇,张令强.调控骨骼形成的信号通路[J].中国生物化学与分子生物学报,2021,37(4):428-436.
[40] WU X,CHEN S,ORLANDO S A,et al.p85alpha regulates osteoblast differentiation by cross-talking with the MAPK pathway[J].Journal of Biological Chemistry,2011,286(15):13512-13521.
[41] 尹文哲,邢成亮,王建业,等.失重下骨碎补总黄酮经ERK通路促成骨细胞增殖研究[J].中医药学报,2017,45(5):45-48.
[42] 张力,徐勃兴,李杰,等.MAPK抑制剂对骨碎补总黄酮促进转基因成肌细胞成骨分化的影响[J].中国组织工程研究,2016,20(51):7622-7627.
[43] 邓传超,尹文哲,杜挺媛,等.失重下骨碎补总黄酮经JNK通路促成骨细胞增殖研究[J].哈尔滨医科大学学报,2016,50(5):403-406.
[44] 王丹华. 仙灵骨葆胶囊中组织蛋白酶K靶向性抗骨质疏松化学成分筛选[J].中华骨质疏松和骨矿盐疾病杂志,2019,12(5):488-493.
[45] 史晓林,李春雯,孙金谞,等.骨碎补总黄酮对去卵巢大鼠组织蛋白酶K的影响[J].中华骨质疏松和骨矿盐疾病杂志,2010,3(4):285-290.
[46] 周灵通,黄凯,宋敏,等.Notch信号通路在骨重建过程中的双向调节作用[J].中国骨质疏松杂志,2019,25(7):1015-1020.
[47] 韩亚力,罗奕,曾佳学.骨碎补总黄酮基于Notch信号通路改善骨质疏松的作用及机制研究[J].中国免疫学杂志,2018,34(2):261-266.
[48] 方霁,杨丽,沈家珍,等.骨碎补总黄酮对去卵巢骨质疏松大鼠骨内谷氨酸信号Glu、mGluR5以及EAAT_1的影响[J].中国生化药物杂志,2014,34(2):10-12,16.
[49] 吕辰鹏. 骨碎补总黄酮对去卵巢大鼠骨组织GABA信号通路的影响[D].广州:暨南大学,2011.
[50] 曾强成,张西正,刘洪玲,等.谷氨酸信号通路及其在骨力学信号转导通路中潜在的功能[J].生物技术通讯,2007(5):827-829.