GnRH的生物学作用及其类似物在马属动物中的应用研究进展

doi:10.12160/j.issn.1672-5190.2023.06.010

畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (6): 82-87.doi: 10.12160/j.issn.1672-5190.2023.06.010

GnRH的生物学作用及其类似物在马属动物中的应用研究进展

李泽廷¹, 苏少锋¹, 赵俊利¹, 孙培陪², 白晋宇³, 吴海青¹

1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
2.内蒙古草原御驴科技牧业有限公司,内蒙古呼和浩特 010020;
3.内蒙古草原同源牧业有限公司,内蒙古呼和浩特 010020

收稿日期:2021-07-28 修回日期:2023-08-31 出版日期:2023-11-30 发布日期:2024-01-11
通讯作者: 吴海青（1983—）,男,副研究员,博士,主要研究方向为马属动物繁育及应用技术。
作者简介:李泽廷（1994—）,女,硕士,主要研究方向为马属动物繁殖机理。
基金资助:
内蒙古自治区呼和浩特市重大科技项目“驴高效繁殖技术研究与示范”（2020-农-重-2）; 内蒙古自治区农牧业创新基金项目“牛羊品种育繁技术研究与示范——基于GEBV技术的乳用驴核心群建立及选育技术研究（2022CXJJM02）”

Research Progress in Biological Roles of GnRH and Application of Its Analogues in Equine Animals

LI Zeting¹, SU Shaofeng¹, ZHAO Junli¹, SUN Peipei², BAI Jinyu³, WU Haiqing¹

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Inner Mongolia Caoyuan Yulyu Science and Technology Animal Husbandry Co.,Ltd.,Hohhot 010020,China;
3. Inner Mongolia Caoyuan Tongyuan Animal Husbandry Co.,Ltd.,Hohhot 010020,China

Received:2021-07-28 Revised:2023-08-31 Online:2023-11-30 Published:2024-01-11

摘要/Abstract

摘要： 繁殖率低是制约马属动物产业发展的瓶颈,繁殖调控技术不完善也是导致该产业发展缓慢的主要因素。促性腺激素释放激素（gonadotropin-releasing hormone,GnRH）是下丘脑分泌产生的神经激素,在下丘脑-垂体-性腺轴通路中起关键作用。GnRH可以有效促进马属动物卵泡发育及排卵。GnRH是具有十肽结构的化合物,通过改变十肽结构中不同位置的氨基酸合成不同的GnRH类似物。GnRH及其类似物可通过刺激卵泡刺激素（follicle-stimulating hormone,FSH）和黄体生成素（luteinizing hormone,LH）的分泌、抑制雌激素受体的合成影响动物繁殖性能。GnRH及其类似物已被证明可提高马属动物的繁殖性能。对GnRH和促性腺激素释放激素受体（gonadotropin-releasing hormone receptor,GnRHR）的结构特点、生物学功能以及GnRH类似物在马属动物繁殖方面的应用研究进展进行综述,以期为探究GnRH生物学作用的分子机制以及GnRH类似物在马属动物繁殖调控中的科学应用提供参考。

关键词: 促性腺激素释放激素, 促性腺激素释放激素受体, 促性腺激素释放激素类似物, 生物学功能, 马属动物, 繁殖调控

Abstract: The low reproductive rate is a bottleneck that restricts the development of the equine animal industry, and the inadequate technology for reproductive regulation is the primary cause for the slow growth of the industry. Gonadotropin-releasing hormone （GnRH）, a neurohormone released by the hypothalamus, plays essential roles in the hypothalamic-pituitary-gonadal-axis pathway. GnRH can effectively promote follicular development and ovulation in equine animals. It is a decapeptide-containing compound that can be synthesized into many GnRH analogues by varying the amino acids at different locations within the decapeptide structure. GnRH and its analogues affect animal reproductive performance by stimulating the secretion of follicle-stimulating hormone （FSH） and luteinizing hormone （LH）, and inhibiting the synthesis of estrogen receptors. It has been demonstrated that GnRH and its analogues could improve the reproductive performance of equine animals. This article reviews the structural characteristics and biological functions of GnRH and gonadotropin-releasing hormone receptor （GnRHR） as well as the research progress in the application of GnRH analogues in the reproduction of equine animals, in hoping to provide references for exploring the molecular mechanisms behind the biological effects of GnRH and the scientific application of GnRH analogues in the reproductive regulation of equine animals.

Key words: gonadotropin-releasing hormone, gonadotropin-releasing hormone receptor, gonadotropin-releasing hormone analogue, biological function, equine animal, reproductive regulation

中图分类号:

S821.3

李泽廷, 苏少锋, 赵俊利, 孙培陪, 白晋宇, 吴海青. GnRH的生物学作用及其类似物在马属动物中的应用研究进展[J]. 畜牧与饲料科学, 2023, 44(6): 82-87.

LI Zeting, SU Shaofeng, ZHAO Junli, SUN Peipei, BAI Jinyu, WU Haiqing. Research Progress in Biological Roles of GnRH and Application of Its Analogues in Equine Animals[J]. Animal Husbandry and Feed Science, 2023, 44(6): 82-87.

参考文献

[1] SCHMID T,BOEHM U,BRAUN T.GnRH neurogenesis depends on embryonic pheromone receptor expression[J].Molecular and Cellular Endocrinology,2020,518:111030.
[2] SCHALLY A V, BLOCK N L, RICK F G.Discovery of LHRH and development of LHRH analogs for prostate cancer treatment[J].The Prostate,2017,77(9):1036-1054.
[3] 徐元青,王建林,邵宝平.促性腺激素释放激素(GnRH)结构及调控的研究进展[J].畜牧与饲料科学,2014,35(12):31-34.
[4] 范文静,李纯锦,陈璐,等.促性腺激素释放激素的研究和应用进展[J].黑龙江动物繁殖,2020,28(4):23-30.
[5] DESAULNIERS A T, CEDERBERG R A, LENTS C A, et al.Expression and role of gonadotropin-releasing hormone 2 and its receptor in mammals[J].Frontiers in Endocrinology,2017,8:269.
[6] CHENG C K, LEUNG P C K. Molecular biology of gonadotropin-releasing hormone (GnRH)-Ⅰ, GnRH-Ⅱ, and their receptors in humans[J].Endocrine Reviews,2005,26(2):283-306.
[7] 林浩然. 促性腺激素释放激素(GnRH)结构与功能及其受体的进化发展[J].中山大学学报(自然科学版),2004,43(6):1-5.
[8] WHITE S A, BOND C T, FRANCIS R C, et al.A second gene for gonadotropin-releasing hormone: CDNA and expression pattern in the brain[J].Proceedings of the National Academy of Sciences of the United States of America,1994,91(4):1423-1427.
[9] MILLAR R P.GnRHs and GnRH receptors[J].Animal Reproduction Science,2005,88(1/2):5-28.
[10] 叶丹,潘建伟,廖鸣娟,等.促性腺激素释放激素的结构及其生物学功能[J].生物化学与生物物理进展,2003,30(1):49-53.
[11] HUANG W Q, YAO B, SUN L, et al.Immunohistochemical and in situ hybridization studies of gonadotropin releasing hormone (GnRH) and its receptor in rat digestive tract[J].Life Sciences,2001,68(15):1727-1734.
[12] DIANA F, MATTEO T, ALESSANDRA R, et al.CG is more effective than the GnRH agonist Buserelin for inducing the first ovulation of the breeding season in mares[J].Equine Veterinary Journal,2021,54(2):306-311.
[13] WOLFSDORF K E, FEDORKA C E, LU K J, et al.The effect of Buserelin on reproductive performance in the transitional and anestrous mare[J].Journal of Equine Veterinary Science,2018,66:98.
[14] CHASON R J, KANG J H, GERKOWICZ S A, et al.GnRH agonist reduces estrogen receptor dimerization in GT1-7 cells: Evidence for cross-talk between membrane-initiated estrogen and GnRH signaling[J].Molecular and Cellular Endocrinology,2015,404:67-74.
[15] CASTRO T, OLIVEIRA F A, SIDDIQUI M A R, et al. Stimulation of LH, FSH, and luteal blood flow by GnRH during the luteal phase in mares[J].Theriogenology,2016,85(4):740-746.
[16] 龚建文,黄峰,陶斌.浅析GnRH对提高母猪产仔数及存活数的作用[J].黑龙江动物繁殖,2011,19(3):49.
[17] 刘闯,李楠,魏巧莉,等.促性腺激素释放激素及其类似物在母猪繁殖中应用的研究进展[J].中国畜牧兽医,2019,46(2):512-518.
[18] MONTOVAN S M, DAELS P P, RIVIER J, et al.The effect of a potent GnRH agonist on gonadal and sexual activity in the horse[J].Theriogenology,1990,33(6):1305-1321.
[19] BRINSKO S P, SQUIRES E L, PICKETT B W, et al.Gonadal and pituitary responsiveness of stallions is not down-regulated by prolonged pulsatile administration of GnRH[J].Journal of Andrology,1998,19(1):100-109.
[20] SIEME H, TROEDSSON M H T, WEINRICH S, et al. Influence of exogenous GnRH on sexual behavior and frozen/thawed semen viability in stallions during the non-breeding season[J].Theriogenology,2004,61(1):159-171.
[21] 宋亚攀,孙丽萍,宋洛文,等.GnRH类似物及其在动物繁殖上的应用[J].中国奶牛,2010(3):22-26.
[22] FALOMO M E, NORMANDO S, ZANIBELLATO E, et al.Sexual behavior and serum testosterone concentration in stallions treated with slow-release implants of Deslorelin acetate[J].Journal of Veterinary Behavior,2013,8(4):278-284.
[23] GAUTIER C, SCHMIDT K, AURICH J, et al.Effects of implants containing the GnRH agonist Deslorelin on testosterone release and semen characteristics in Shetland stallions[J].Animal Reproduction Science,2018,195:230-241.
[24] BOAKARI Y L, FERREIRA J C, CANESIN H S, et al.Influence of two ovulation-inducing agents on the pituitary response and follicle blood flow in mares[J].Theriogenology,2017,100:95-99.
[25] YANG F, LI N, LIU B, et al.Practical protocols for timed artificial insemination of jennies using cooled or frozen donkey semen[J].Equine Veterinary Journal,2021,53(6):1218-1226.
[26] FINAN S A, LAMKIN E L, MCKINNON A O.Comparative efficacy of BioRelease Deslorelin injection for induction of ovulation in oestrus mares:A field study[J].Australian Veterinary Journal,2016,94(9):338-340.
[27] FARQUHAR V J, MCCUE P M, NETT T M, et al.Effect of Deslorelin acetate on gonadotropin secretion and ovarian follicle development in cycling mares[J].Journal of the American Veterinary Medical Association,2001,218(5):749-752.
[28] JOHNSON C A, THOMPSON D L, KULINSKI K M, et al.Prolonged interovulatory interval and hormonal changes in mares following the use of Ovuplant^TM to hasten ovulation[J].Journal of Equine Veterinary Science,2000,20(5):331-336.
[29] JOHNSON C,MCMEEN S,THOMPSON D.Effects of multiple GnRH analogue (Deslorelin acetate) implants on cyclic mares[J].Theriogenology,2002,58(2/3/4):469-471.
[30] DORDAS-PERPINYA M, NORMANDIN L, DHIER T, et al.Single injection of triptorelin or Buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG[J].Reproduction in Domestic Animals,2020,55(3):374-383.
[31] NEWCOMBE J R, CUERVO-ARANGO J.Induction of multiple ovulation in mares following multiple treatment with low dose Buserelin[J].Journal of Equine Veterinary Science,2018,66:95-96.
[32] HUIRNE J A, LAMBALK C B.Gonadotropin-releasing-hormone-receptor antagonists[J].The Lancet,2001,358(9295):1793-1803.
[33] COCCIA M E, COMPARETTO C, BRACCO G L, et al.GnRH antagonists[J].European Journal of Obstetrics, Gynecology, and Reproductive Biology,2004,115(Suppl 1):44-56.
[34] REICHMAN D, ROSENWAKS Z.GnRH antagonist-based protocols for in vitro fertilization[M]// WASSARMAN P M. Human Fertility:Methods and Protocols. New York:Humana Press,2014:289-304.
[35] 谭秀群, 丘映. 促性腺激素释放激素拮抗剂在辅助生殖技术中的应用研究进展[J].医学综述,2012,18(18):2937-2940.
[36] DAL PRATO L, BORINI A.Use of antagonists in ovarian stimulation protocols[J].Reproductive BioMedicine Online,2005,10(3):330-338.
[37] ALBANO C, FELBERBAUM R E, SMITZ J, et al.Ovarian stimulation with HMG:Results of a prospective randomized phase ⅢEuropean study comparing the luteinizing hormone-releasing hormone (LHRH)-antagonist Cetrorelix and the LHRH-agonist Buserelin[J].Human Reproduction,2000,15(3):526-531.
[38] BRIANT C, OTTOGALLI M, MOREL M, et al.Use of a GnRH antagonist, Antarelix, associated or not with hCG, to control ovulation in cyclic pony mares[J].Domestic Animal Endocrinology,2003,24(4):305-322.
[39] WATSON E D, PEDERSEN H G, THOMSON S R M, et al. Control of follicular development and luteal function in the mare:Effects of a GnRH antagonist[J].Theriogenology,2000,54(4):599-609.
[40] EVANS M J, KITSON N E, ALEXANDER S L, et al.Effectiveness of an antagonist to gonadotrophin releasing hormone on the FSH and LH response to GnRH in perifused equine pituitary cells, and in seasonally acyclic mares[J].Animal Reproduction Science,2002,73(1/2):37-51.
[41] FORTIER G, VIDAMENT M, DECRAENE F, et al.The effect of GnRH antagonist on testosterone secretion, spermatogenesis and viral excretion in EVA-virus excreting stallions[J].Theriogenology,2002,58(2/3/4):425-427.
[42] HINOJOSA A M, BLOESER J R, THOMSON S R M, et al. The effect of a GnRH antagonist on endocrine and seminal parameters in stallions[J].Theriogenology,2001,56(5):903-912.

GnRH的生物学作用及其类似物在马属动物中的应用研究进展

Research Progress in Biological Roles of GnRH and Application of Its Analogues in Equine Animals

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	戴伶俐, 王娜, 白帆, 张帆, 宋越, 张月梅, 达来宝力格, 李晓艳, 王根云, 赵世华. 9株绵羊肺炎支原体内蒙古分离株P113基因克隆及蛋白序列分析[J]. 畜牧与饲料科学, 2022, 43(6): 1-5.
[2]	王建文, 孟军, 姚新奎. 基于“产学研”合作的马属动物生产学课程教学探讨[J]. 畜牧与饲料科学, 2020, 41(3): 125-128.
[3]	梁新晓,陈丽丹,艾琴,刘小艳,方炳虎. 凝结芽胞杆菌的生物学功能及其在畜禽生产中的应用研究进展[J]. 畜牧与饲料科学, 2019, 40(4): 47-51.
[4]	柳杭,韩越,姜怀志. 脂蛋白分解酶基因(LPL)及其与家畜胴体品质的关系[J]. 畜牧与饲料科学, 2019, 40(3): 60-65.
[5]	吴海青, 萨初拉, 苏少锋, 赵俊利, 刘永斌, 李玉荣. hCG促进马属动物排卵的研究进展[J]. 畜牧与饲料科学, 2019, 40(11): 57-60.
[6]	邢雪静, 尹国安. GnRH类似物疫苗免疫去势效果的影响因素[J]. 畜牧与饲料科学, 2019, 40(11): 61-64.
[7]	吴海青,吴学良,布仁巴特,苏依勒,乌兰巴特尔,李玉荣. 马匹繁殖率的主要影响因素[J]. 畜牧与饲料科学, 2017, 38(6): 21-21.
[8]	王丽思,乔文军,刘心宽,彭永强,高固生. 葱属植物的生物学功能及其在养殖业中的应用[J]. 畜牧与饲料科学, 2016, 37(8): 51-51.
[9]	李贞. 卵磷脂的生物学功能及其应用[J]. 畜牧与饲料科学, 2016, 37(2): 94-94.
[10]	王浩波. 抗菌肽的研究进展及其在动物生产中的应用[J]. 畜牧与饲料科学, 2015, 36(1): 55-55.
[11]	陈建兴,李艳龙,张彦,陈海伟,赵冰. 根据线粒体3个基因确定马属动物系统发育关系[J]. 畜牧与饲料科学, 2014, 35(9): 21-21.
[12]	赵玉清. 马属动物破伤风的诊治[J]. 畜牧与饲料科学, 2014, 35(7): 149-149.
[13]	徐元青,王建林,邵宝平. 促性腺激素释放激素（GnRH）结构及调控的研究进展[J]. 畜牧与饲料科学, 2014, 35(12): 31-31.
[14]	马燕芬,红梅,格根塔娜,张春华. 紧密连接的分子结构、生物学功能及其信号调控[J]. 畜牧与饲料科学, 2013, 34(7): 39-39.
[15]	徐晨晨,王珂. 微量元素铜在畜禽营养中的研究进展[J]. 畜牧与饲料科学, 2013, 34(10): 21-21.