Abstract: One strain W7 with high acid production was obtained from the intestinal of chicken and pickle by using the conventional microbiology method. The strain W7 was identified by morphological observation, physiological biochemical test and 16S rRNA gene sequence analysis, and biological characteristics of the strain W7 was studied. Results showed that the strain W7 was identified as Lactobacillus plantarum. The amount of W7 reached the maximum at 37 ℃ for 22 h. The strain W7 had strong resistance to acid, and the survival rate still reached 100% at pH 2.0. The strain W7 could grow when the concentration of bile salt was 0.30%. The strain had good antibacterial effect on some pathogenic bacteria, and the adhesion rate was 79.9%.