Abstract: 360 one-day old yellow-feathered broilers were randomly divided into four groups to drink with 0, 0.01, 0.05 and 0.10 mg/L glycyl-glutamine dipeptide （made with cool boiled water） respectively for 41 days. The content of TG in the liver was determined by commercial TG kit. By using semi-quantitative RT-PCR,mRNA expression level of ACC and PPAR ot were detected. The results showed that Gly-Gln dipetide dose-dependently decreased the expression level of A CC mRNA in the liver and that in 0.10 mg/L Gly-Gln dipetide group had significant difference with control group （P〈0.05）. Gly-Gln dipetide could significantly increased the expression level of PPAR ot mRNA and that in 0.05 mg/L Gly-Gln dipetide group had significant difference with control group（P〈0.05）. Gly-Gln dipetide had the trend of increasing the TG content in the liver, but the difference was not significant （P 〉0.05）. Therefore Gly-Gln dipetide could decrease the synthesis of TG in the liver and promote metabolism, so as to reduce body fat deposition.