Abstract: This study aimed to assess the protective effect of Echinops latifolius polysaccharide B （ETPB） against oxidative damage in INS-1 cells induced by H2 O2 . The cultured INS-1 cells were used to prepare an in vitro oxidative damage model with treatment of 50 滋 mol/L H2 O2 for 24 hours by determination of cells viability using MTT method; the maximum safety concentration of ETPB for INS-1 cells was determined as 250 滋 g/mL; intervention of 15.6, 31.2 and 62.5 滋 g/mL of ETPB for 48 hours ahead of oxidative damage model preparation significantly increased the viability and SOD activity and decreased the MDA concentration in INS-1 cells （P〈0.05 or P〈0.01）. Our results demonstrate the protective effect of ETPB against oxidative damage in INS-1 cells.