Abstract: [Objective] To evaluate the safety of Panax notoginseng saponins (PNS) for clinical use.[Method] A total of 40 Kunming mice were randomly divided into four groups with 10 mice in each group. Mice in the three experimental groups were intraperitoneally injected with 11.34, 56.70, 113.40 mg/(kg·BW) PNS once a day for 14 consecutive days;mice in the blank control group were intraperitoneally injected with 0.3 mL of normal saline for 14 consecutive days. The body weight, organ index (liver, spleen and kidney index) and blood biochemical indexes of mice in each group were measured and compared;the liver, spleen and kidney tissues of mice in each group were taken to prepare histopathological slide, and the pathological changes of the above tissues were observed by microscopy.[Result] There was no significant difference in body weight between the three PNS treatment groups and the blank control group (P>0.05);the spleen index of 11.34 mg/(kg·BW) PNS group was significantly lower than that of the blank control group (P<0.05);the aspartate aminotransferase activity and serum creatinine content of 113.40 mg/(kg·BW) PNS group were significantly lower than that of the blank control group (P<0.05), while blood urea nitrogen content was significantly higher than that of the blank control group (P<0.05);the histopathological observation demonstrated that PNS had no obvious damage to liver, spleen and kidney.[Conclusion] PNS is actually non-toxic, but its long-term use can produce nephrotoxicity, suggesting that the clinical use of PNS should not be excessive.