Abstract: The aim of this study was to explore the effect of Sophora subprosrate polysaccharide on the secretion of inflammatory factors in vivo and in vitro in mice infected with porcine circovirus 2 （PCV2）. An in vitro inflammation model was established after mouse splenic lymphocytes were infected with PCV2, the cells were treated with Sophora subprosrate polysaccharide at different concentrations （100, 200, 400 μg/mL）, and the secreted IL-1β, IL-8, MCP-1 levels by lymphocytes and intracellular COX-1 activity were measured by ELISA method; an oxidative stress model was established after infecting Kunming mice with PCV2 in vivo, intraperitoneal injections of low, medium and high concentrations [100, 200, 400 mg/（kg·BW）] Sophora subprosrate polysaccharide were given and ELISA method was used to determine the secretion level of inflammatory factors IL-1β, IL-8, MCP-1 and activity of COX-1 in mouse spleen, lung tissue and serum. The results showed that, compared with PCV2 infection model cells, the secreted inflammatory factors IL-1β, IL-8, MCP-1 and intracellular COX-1 activity in mouse splenic lymphocytes treated with different concentrations of Sophora subprosrate polysaccharide were reduced at different levels, and 400 μg/mL Sophora subprosrate polysaccharide had the best effect （P<0.01）; after intraperitoneal injection of PCV2 with different concentrations of Sophora subprosrate polysaccharide in mice, it could inhibit the spleen, lung tissue and serum levels of inflammatory factors IL-1β, IL-8, MCP-1 and COX-1 activity, and the high concentration [400 mg/（kg·BW）] Sophora subprosrate polysaccharide had the best effect （P<0.01）. In summary, Sophora subprosrate polysaccharide can inhibit the secretion of inflammatory factors in mice infected with PCV2, thereby exerting an anti-inflammatory effect.

Key words: Sophora subprosrate polysaccharide, PCV2, inflammatory reaction