Abstract: In order to scientifically understand and systematically evaluate the safety and antioxidant activity of Pseudostellaria heterophylla extract at the cellular level, the associated published literatures indexed in domestic and foreign databases were retrieved according to the PICOS search strategy, and Stata software was used for Meta evidence-based statistical analysis. A total of 18 studies in 11 articles were included, and the total samples were 482. Meta-analysis demonstrated that the Pseudostellaria heterophylla test group had no significant difference in in vitro cytotoxicity tests compared with control group [SMD=0.22, 95% CI （-0.22, 0.66）, P=0.219>0.05]; Pseudostellaria heterophylla significantly improved the cell survival rate [SMD=0.96, 95% CI （0.15, 1.77）, P<0.05], significantly decreased the apoptosis rate [SMD=-2.38, 95% CI （-3.21,-1.54）, P<0.01], significantly elevated the intracellular superoxide dismutase （SOD） activity [SMD=1.65, 95% CI（0.97, 2.32）, P<0.01], and significantly reduced the intracellular malondialdehyde （MDA） content [SMD=-1.99, 95% CI （-2.68 , -1.29）, P<0.01] in the in vitro tests; the systematic description result of inhibiting hemolysis of erythrocytes in vitro was positive, the sensitivity analysis result was robust, and the publication bias was weak. Based on the existing literatures, Pseudostellaria heterophylla extract has good safety and antioxidant activity at the cellular level.

Key words: Pseudostellaria heterophylla, in vitro cell model, safety, antioxidant activity, systematic evaluation