Abstract: [Objective] The deamination and nitrogen removal effects of four yeast strains and their combinations with spore bacteria on the wastewater from blister manure were investigated. [Method] Taking the removal rates of ammonia nitrogen and ammonia gas as measurement indicators, this study investigated the deamination effect on piggery wastewater containing blisters when inoculated with four yeast strains: Saccharomyces cerevisiae ZP, Sac. cerevisiae X1, Candida ethanolica JMJ, and Can. ethanolica HJ, at a dosage of 1%. The deamination and nitrogen removal efficiencies of combined inoculations involving Candida ethanolica strains JMJ and HJ with Bacillus megaterium strain LB and Bacillus licheniformis strain WL, at an inoculation concentration of 1.5%, were evaluated using wastewater from blister manure as the medium. This study aimed to determine the optimal combination of yeast and bacillus strains for effective wastewater treatment. The deamination and nitrogen removal efficacy of co-inoculating Candida ethanolica strain JMJ and Bacillus megaterium strain LB at inoculation concentrations of 0.5%, 1%, and 1.5% in wastewater from blister manure was investigated to determine the optimal combination ratio of yeast and bacillus. [Result] When the four yeast strains were individually employed to treat sewage, all demonstrated the capability to remove ammonia. The relative removal rate of ammonia ranged from 17.90% to 75.41% within 6 to 72 hours post-inoculation. When yeast strains are co-cultured with bacillus, the combination of Candida ethanolica JMJ and Bacillus megaterium LB demonstrates superior performance in the removal of ammonia and ammonia nitrogen, exhibiting long-term effectiveness. The relative removal rate of ammonia gas remains at 38.53% - 41.45% within the initial 3 to 6 hours and reaches 100.00% within 12 to 72 hours. The relative removal rate of ammonia nitrogen is maintained between 17.29% and 25.65% over a period of 24 to 72 hours. In terms of both removal efficiency and stability, an inoculation amount of 1.50% outperforms those of 0.50% and 1.00%. [Conclusion] The synergistic application of yeast and bacillus for the ammonia nitrogen treatment of flushing manure wastewater demonstrates superior efficiency compared to the use of yeast alone. This research offers valuable theoretical insights and practical recommendations for the effective management and resource utilization of ammonia nitrogen in flushing manure wastewater.

Key words: bubble manure sewage, ammonia nitrogen, yeast, Bacillus, removal