Abstract: [Objective]To establish an identification and content determination method for picfeltarraeninⅠA in Picria fel-terrae extracts. [Methods]The qualitative identification of picfeltarraenin ⅠA was carried out with thin layer chromatography（TLC）method; high performance liquid chromatography（HPLC） method was used to determine the content of picfeltarraenin ⅠA in Picria fel-terrae extracts, and the chromatography conditions were as followed： the chromatographic column was Waters Symmetry C18（4.6 ×250 mm, 5 μm）, the mobile phase was acetonitrile-water（35 ∶65）, the velocity was 1.0 m L/min, the detecting wavelength was 264 nm, the column temperature was 35 ℃, and the injection volume was 20 μL. [Results]The TLC method showed high specialization for identification of picfeltarraenin ⅠA . A good linear relationship was observed in the mass concentration range of 20.06-104.10 μg/m L（R2=0.9992）;the average recovery rate was 99.90%, and the RSD was 0.53 %.[Conclusion]The established method in this study has good specialization in qualitative analysis and has high accuracy in quantitative analysis and can be used in quality control of picfeltarraeninⅠA in Picria fel-terrae extracts.