Abstract: This study was conducted to evaluate the effect of a traditional Chinese medicine compound ZD preparation on the expression of IL-1β in muscle of closed soft tissue injured mice. The soft tissue injured model in mice was prepared by the reported methods. The mice were treated by ZD preparation and positive control drugs at the 2ndday after modeling, but the mice in healthy group and model group received no treatment. The health situation and pathological changes of the mice were observed by naked eyes during the early stage of modeling and therapy. On days of 2 and 7 of modeling, the immunohistochemistry assay was employed to evaluate the expression of IL-1β in the injured muscle tissue. The results showed that the symptoms of depression and claudication were observed in mice of model group, and swelling as well as subcutaneous congestion was also observed in the injured muscle tissue. After treatment, the mice with soft tissue injuries achieved remission of symptoms. At the 2nd day of modeling, the expression of IL-1β in the injured muscle tissue of mice in model group, positive control drugs treatment group and ZD preparation treatment group was significantly higher than that in the normal muscle tissue of mice in healthy group（P〈0.05）. At the 7^th day of modeling, significantly reduced expression of IL-1β in the injured muscle tissue of mice in positive control drugs treatment group and ZD preparation treatment group was found compared with that in the normal muscle tissue of mice in healthy group（P〈0.05）. The combined data suggests that ZD preparation inhibits the inflammation caused by closed soft tissue injuries in mice by reducing the expression of IL-1β.