Using the frozen embryo transfer technology, frozen embryo transfer was made on two batches of 371 Australia Merino fine-wool sheep receptors in 2007（266 sheep） and 2008 （105 sheep）. And 106 lambs of Australia Merino fine-wool sheep were produced （70 in 2007 and 36 in 2008）, which included 60 male lambs and 46 female lambs. The conception rate of male and female lambs were 26.32% and 37.14% respectively. And the birth weight of male and female lambs were （4.73 ± 0.41） kg and （4.54 ± 0.43） kg, （4.60 ± 0.68） kg and （4.45 ± 0.55） kg respectively. After investigation and identification,it was found that 106 lambs were obtained by using frozen embryo transfer technology and 106 pure Australia Merino fine-wool sheep were cultured. Thus research supplied the number of high-quality Australia Merino fine-wool sheep, greatly increased the number of pure Australia Merino fine-wool sheep, saved the introduction cost, shorten the generation intervals and breeding cycle of Australia Merino fine-wool sheep, which accelerated the reproductive speed of excellent breeders and extended rapidly the scientific and technological effects of seed sources.

Based on conventional meteorological data, satellite images and numerical weather prediction products and other information, a strong convective precipitation processes appeared in Tongliao City on 15-16 July, 2011 was analyzed. Result showed that enabling large-scale circulation, abundant water vapor, the existence of high-altitude jet, low-altitude water vapor convergence, strong upward motion and unstable energy role were extremely favorable factors to stimulate the convective precipitation. If some experience and indicators are summaried based on the precipitation process and then are used in forecasting in the future, they would provide some reference for strong convective precipitation forecast and play an important role to improve forecast accuracy.

[Objective] Whether there was tryptase in the mast cell of cashmere goat and effects of different stationary liquids on its normal dyeing and immunohistochemistry dyeing result were studied. [Method] Tryptase existing in some mast cells like jejunum, omasum and lung tissue of cashmere goats which were fixed by Carnoy liquid and neutral formalin （NBF） were determined by the rabbits of mast cell tryptase polyclonal antibody indirect immunoperoxidase technique. Experimental results were analyzed by using Image-Pro Plus image analysis software and artificial counting method, and the morphology and amount of mast cells in different fixed tissues after normal dyeing and immunohistochemistry dyeing were compared so as to judge the effects of Carnoy liquid and NBF on the technique. [Result] The polyclonal antibody β2 and cashmere goat mast cell tryptase could obtain good cross reaction, which suggested that there was tryptase in the mast cell of cashmere goat cytoplasmic granules. Compared with Carnoy liquid, NBF-fixed tissue could reflect the morphology and amount change of mast cells in different fixed tissues effectively. [Conclusion] There are tryptase in the mast cell of cashmere goat cytoplasmic granules. Compared with Carnoy liquid, NBF is more suitable for the normal dyeing and immunohistochemistry dyeing of cashmere goat mast cell.

In order to understand the effect of weanling stress on serum lipid metabolism of piglets, 12 weaned piglets with similar body weight were randomly selected, and serum lipid metabolism indexes of their blood were determined when piglets were weaned, 7 days and 14 days after weaning. Result showed that CHO and TG activities of piglet serum were significantly or very significantly increased, but HDL and LDL activities of serum had no significant change. It is suggested that after piglets were weaned for 14 days, they strengthened to absorb lipid materials, and fat deposition increased, so serum nutrients should be properly added into feed during this stage, and it is helpful to adjust body′s metabolism and nutrition regulation.

The net primary productivity of natural grassland was analyzed based on climate data of many years in Guoluo and Miami model. Results showed that the net primary productivity of natural grassland in Banma County was the highest.

Effects of different top clearances of aseptic brick on some qualities of liquid milk product were studied. Results showed that two kinds of top clearances of aseptic brick had different effects on trace elements and sensory of liquid milk product.

The economic value and present population dynamics of four wild ungulates like Moschus chrysogaster, Cervus elaphus, Pseudois nayaur and Gazella subgutturosa in Helan Mountains were recorded. Endangered reasons of the wild ungulates were analyzed, meanwhile the management suggestions were put forward.

[Objective] The research aimed to study the changes of T-SOD activity and MDA content in the serum of small-tailed Han sheep in perinatal period. [Method] 20 2～5 year-old small-tailed Han sheep were selected, including 10 pregnant ewes and 10 non-pregnant ewes. The blood samples was collected on the 20th, 10th and 1st d before parturition and the 1st, 10th, 20th d after parturition to measure T-SOD activity and MDA content in the serum. [Result] T-SOD activity in the serum of small-tailed Han sheep showed the dynamic change trend of first decrease and gradual increase in the whole perinatal period. T-SOD activity on the 1st day before parturition was lowest and T-SOD in the serum of small-tailed Han sheep in perinatal period was all lower than that in non- pregnant ewes. MDA content in the serum of small-tailed Han sheep in perinatal period showed the dynamic change trend of first decrease and gradual increase in the whole perinatal period. MDA content on the 1st day after parturition was lowest and T-SOD in the serum of small-tailed Han sheep in perinatal period was all higher than that in non- pregnant ewes. [Conclusion] The pregnancy and parturition had some influences on T-SOD activity and MDA content of small-tailed Han sheep.

The application of DNA barcoding technique in medical vector identification was summarized. Compared with the other identification techniques, the DNA barcoding technique could identified species fastly and easily, so the technique will be widely used in medical vector.

The studies on the effects of limiting time on the utilization of grassland in the thickening demonstration region of cashmere goat in Ordos City of Inner Mongolia showed that the average height of vegetation in the limiting time grazing region increased 5 cm, the coverage increased 12%,the density increased 40 plants/m2 and the overground biomass increased by 20%-40%, compared with that in freedom grazing region.

In order to explore the effects of urea on the feed efficiency, rumen fistula sheep was used as test animal to study the effects of urea concentration gradients on the degradability of feed in the sheep rumen and the content changes of main nutrients by using nylon-bag method. Three rumen fistula sheep were selected as test animals. Control group （no urea） and test groups（adding 1%, 2%, 3%, 4% and 5% urea） were set. The content of crude fat,crude fiber,ash,crude protein,calcium and total degradation rate in the 6th , 12th , 24th hour in rumen were measured. The results showed that the feed degradability at the addition level of 2% and 3% was significantly higher than that of control group and 5% urea group, which indicated that suitable amount of urea could promote the utilization rate of feed nutrients. Adding 2% and 3% urea could promote the early-period utilization rate of calcium in feed and adding 3% and 4% urea could promote the early-period utilization rate of ash in the feed.

Using big-leaf cassia in Etuoke Front Banner of Inner Mongolia as test materials and choosing Leymus chinensis and alfalfa as control, the nutritional value of big-leaf cassia was comprehensively assessed by conventional nutritional component analysis, in vitro gas production and fermentation test,two-stage in vitro digestibility test and nylon bag test, etc.. From the conventional nutritional component analysis, the protein content in big-leaf cassia with the height of 0.8-1.2 m was the highest, followed by alfalfa. From the fermentation parameters in vitro,there was no significant difference. From the digestibility in vitro,the protein digestibility of big-leaf cassia with the height of 0.8-1.2 m was lower than that of alfalfa and higher than that of L. chinensis and other two kinds of cassia. From the digestibility in semi-vivo , the protein digestibility of big-leaf cassia with the height of 0.8-1.2 m was lower than that of alfalfa and higher than that of other three kinds of feed. In conclusion, it was found that big-leaf cassia with the height of 0.8-1.2 m was not suitable as fiber feed but fibrous feed.

According to the growth and development characteristics of cows, cows in perinatal period was divided into three stages： early perinatal stage（21 d before parturition）, late perinatal stage（21 d after parturition） and lactation（from the 21st day to the end of lactation）. And the feeding and management program was set up according to the growth characteristics and nutritional demands in three stages. The test results indicated that the feeding program in the dry period and perinatal period could decrease the incidence of retained placenta,milk fever,metritis and ketosis（P0.05）. The milk yield in test group increased 6.08 kg than that of control group, with significant difference （P0.05）. The milk compositions between the two groups had no significant change （P0.05）.

The industrialized research results of the optimization of the technical parameters of synergistic solid state fermentation of producing soybean oligopeptide protein feed with the two kinds of bacteria were summarized. The model was as follows： combination of two kinds of bacteria → liquid state inoculation→ closed fermentation in fermentation pool. The two kinds of bacteria were screened out： Bacillus subtilis and Lactobacillus. The optimized fermentation technical parameters were as follows： the fermentation time of 38 h, pH of 7～8, the initial temperature of （37±1） ℃, the inoculated volume of 2% Bacillus subtilis and 3% Lactobacillus, the feed-water ratio of 2∶1, the substrate composition of 90% soybean meal, 7% wheat bran and 3% maize meal.

60 3-4 years old Zhongwei goat ewes in milk with lambs which had similar body weight, similar lambing period and good health were randomly divided into two groups of A and B, with 30 goats in each group. Results showed that there was no significant difference in the average weight of each month old lamb between A and B groups after five months of feeding.

The practice teaching is an extremely important part of undergraduate education and is an important part to develop innovative spirit and creative ability of students. The construction of Practice Teaching Center of animal science specialty in local colleges was explored and practiced from some aspects of practical teaching position, developing ideas, building programs and other contents.

Test Methodology of Animal Food Hygiene, as the main course of animal and veterinary speciality and animal epidemic prevention and quarantine speciality, has strong professional property and practicality. In order to improve the basic theory, practical skill and food safety system control idea of students to food hygienic analysis, some reform ideals for Test Methodology of Animal Food Hygiene course were put forward from teaching effect. The learning enthusiasm and practice ability of students were explored by using the existing resource, meanwhile the training for strengthening base skill was payed attention, and some effects have been obtained.

The gradual propaganda of information technology in the classroom teaching created good conditions for the teaching reform of various disciplines. Quantitative genetics, which is the product of applying quantitative traits based on statistics and population genetics, is a major professional basic course in agricultural colleges and universities. The significance of the integration of information technology in the teaching of quantitative genetics was firstly analyzed. Then the basic strategies of integrating information technology in the teaching of quantitative genetics were mainly expounded.

In order to provide reference for the construction of animal production kinds excellent curriculum, the concrete enforcement measures of excellent curriculum construction of Swine Production were discussed from five aspects of teaching staff, teaching contents, teaching methods, textbook construction and teaching management.

Meat sheep industry occupies an important position in animal husbandry in Xinjiang, and sheep farming income is the main income for many herdsman. But there are some factors like grassland degradation, lack of development funds, backward mode of production and low level of industrialized, etc. to restrict the development of Xinjiang meat sheep industry. The main constrains were analyzed from the current situation in Xinjiang meat sheep industry, and then policy recommendations were put forward in order to provide basis for local government to make the policy of meat sheep industry.

Based on the investigation on the development situation of animal husbandry cooperative in Jiangsu Province, the form scale, leading subject, regional distribution, business scale, service content, development environment and other aspects of the basic situation in recent years were analyzed, and main characteristics of the development situation were summarized in order to provide the basis for the further development of Jiangsu animal husbandry cooperatives and animal husbandry industry.

The anti-inflammatory and analgesic effect of self-made external Chinese traditional medicine cream were studied, so as to provide clinical basis for the treatment of sports injury by using Chinese medicine. The anti-inflammatory and analgesic effect of external Chinese traditional medicine cream was studied by using the methods of ear-swelling induced by xylene in mice, feet-swelling of mice induced by egg white,hot plate test and the acetic acid writhing test. The results showed that external Chinese traditional medicine cream could obviously inhibit the ear-swelling of mice induced by xylene and feet-swelling of mice induced by egg white（P0.01）, and it could enhance the pain threshold of mice in the hot plate test and the acetic acid writhing test（P0.01）. It was proved that external Chinese traditional medicine cream had good anti-inflammatory and analgesia effects.

With the rapid development of artificial turtle culture, all kinds of turtle diseases occurred in a large scale, which brought severe economic damages for culturists. The common diseases of Pelodiscus sinensis and their prevention and treatment were mainly introduced, so as to provide reference for culturists.

Sheep red blood cell treated with tannic acid and glutaraldehyde was sensitized by Mycoplasma ovipneumoniae isolates to prepare the test antigen. The indirect hemagglutination test was made on several kinds of serum through the virus attack to detect its cross reactivity. Through detection,Mycoplasma ovipneumoniae isolates could cause symptoms of pneumonia bacteria without cross reactivity with Pasteurella multocida and Pasteurella multocida etc. .

To discuss the clinical application method of swine gastrotomy, the experiment was made to use gastric operation to take out hair tuft and hair bulb from their gastral cavity of diseased swine. After fasting for more than 24 hours and having narcosis,four old pigs were successfully operated to take out hair tuft and hair bulb in their stomach.The success rate of operation was 100%. The results showed that gastrotomy was a fast and efficient method to clear out foreign matter in gastral cavity. To ensure the success rate of the operation, the whole operation process must be strict and careful postoperative nursing was needed.

Pseudomonas aeruginosa is a conditional pathogenic bacteria existing in environment and normal livestock and poultry body. In recent years, with the deepening of animal disease research, more and more Pseudomonas aeruginosa infections have been reported. In order to lay the foundation for the control of Pseudomonas aeruginosa infection, research advances on Pseudomonas aeruginosa were reviewed from some aspects of etiology, epidemiology, pathogenesis and clinical in this paper.