In order to evaluate the effect of the protein in egg yolk samples on the determination results of cholesterol in duck egg,direct kit method and indirect kit method were used to determine the cholesterol content of duck egg sold in market and the determination results of the two methods were compared. The results showed that there was significant correlation between the determined value of direct kit method and that of indirect kit method, and the determined value of direct kit method was higher than that of indirect kit method.

The diversity of lactic acid bacteria flora in piglets′ intestinal tract in early stage of weaning was liable to change, which made the body of piglets to be sensitive. The probiotics and oligosaccharides were able to promote the recovery of the diversity of lactic acid bacteria flora in piglets′ intestinal tract and to increase the defense of piglets to the intestinal diseases. Different species lactic acid bacteria showed varied ability, which provide new ideas for the screening of functional lactic acid bacteria.Additionally, different oligosaccharides could be fermented by varied lactic acid bacteria. Therefore, the combined application of probiotics and oligosaccharides was expected to be scientifically selected.

In this study, a total of 40 A-Wang sheep were selected in A-wang township of Gongjue County of Changdu Region of Tibet Autonomous Region to determine their physiological and biochemical index in blood. The results showed that the respiration rate, pulse rate and body temperature of A-Wang sheep were in the normal range, and the number of erythrocytes and serum protein content were higher than the normal value. It was indicated that the incensement of certain physiological and biochemical index in blood of A-Wang sheep was caused by their adaptability to the high altitude and low oxygen pressure environment.

The aim of this study was to provide basis for the further understanding of the functions of vsp Y1 and vsp Y2 genes of Mycoplasma bovis Inner Mongolia isolates（NM2012）. Two sets of primers were designed based on the published vsp Y1 and vsp Y2 genes of Mycoplasma bovis and used to amplify the vsp Y1 and vsp Y2 genes of NM 2012 isolate. The obtained sequences were cloned and sequenced. The nucleic acid sequence of vsp Y1 and vsp Y2 genes of NM 2012 isolate were compared with that of the related published genes respectively to identify their similarity. The similarity with other published or deduced amino acid,conserved domain, trans-membrane structure, signal peptide, lipoprotein and epitope of the amino acid sequences deduced by vsp Y1 and vsp Y2 genes were predicted by using online bioinformatics tools, respectively. The results showed that the size of vsp Y1 and vspY2 genes of NM 2012 isolate were 1 029 and 804 bp, respectively, and both of them contained complete open reading frames（ORF）, encoding 342 and 267 amino acids, respectively. The similarity of nucleic acid sequence between vsp Y1 gene of NM 2012 isolate and others related genes ranged from 99.4% to 100%, and that of the deduced amino acids sequence ranged from 98.6% to 99.7%. The similarity of nucleic acid sequence between vsp Y2 gene of NM 2012 isolate and others related genes were 100%. Based on the bioinformatics analysis results obtained in this study, it could be deduced that vsp Y1 was a potential virulence factor.

In order to investigate the effect of tricin on immunological indicators of AA broiler, a total of 120 AA broilers were selected and randomly divided into four groups. The control group was fed with basal diet. The basal diet of experimental groupⅠ, Ⅱ and Ⅲ were supplemented with 150, 300 and 450 mg/kg tricin, respectively. Ten broilers were chosen from each group in28 th, 35 thand 42thd to determine their serum globulin content. Meanwhile, the bursa of Fabricius and spleen of the selected broilers were collected and weighed to calculate their immune organs index, and the proliferation ability of T and B lymphocyte were determined by using MTT colorimetry. The results showed that the index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 28 d（P〈0.05）. The serum globulin level and immune organs index of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 28 d（P 0.05）. The serum globulin level, immune organs index and the proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 35 d（P〈0.05）. The serum globulin level, spleen index and the proliferation ability of T and B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 35 d（P〈0.05 or P〈0.01）. The index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 and 450 mg/kg of tricin for 42 d（P〈0.01）, respectively. The proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 42 d（P〈0.05）. It was indicated that a series of immunological indicators of AA broiler were increased when they were fed with the basal diet supplemented with tricin, and the optimum adding quantity was 450 mg/kg.

The higher air temperature and less and uneven distribution precipitation in Chifeng of Inner Mongolia from December2014 to middle February 2015 had an adverse effect on the normal animal husbandry production in local pasturing area. Based on the analysis of returning green stage of herbage in natural grassland and prophase climatic conditions, the returning green stage of herbage in natural grassland in Chifeng was forecast. The results showed that the returning green stage of herbage in natural grassland in Chifeng in 2015 would emerged from late April to early May, which would be 3-6 d earlier than former years.

In order to evaluate the influence of warm shed feeding manner on weight and wool production performance of Ordos fine wool sheep in cold season, the selected basic ewe, reserved ewe, gimmer hogg and ram hogg were randomly divided into experimental group and control group, respectively. All of the experimental groups fed inside the warm shed at night from December 7 of 2012 to January 31 of 2013, whereas the control groups fed outside the warm shed at the same time. The daily temperature inside and outside the warm shed during the warm shed experiment in cold season were recorded and the changes of body weight of experimental groups and control groups in different sheep flocks were determined and analyzed. After the warm shed experiment was finished, all of the sheep fed to June of 2013. At the end of the experiment, the yield, length and fineness of wool of experimental groups and control groups in basic ewe, reserved ewe and gimmer hogg were determined and analyzed,respectively. The results showed that during the warm shed experiment in cold season, the average temperature at night inside the warm shed was 9.76 ℃ higher than that outside the warm shed, and the minimum temperature inside the warm shed was 14.10 ℃higher than that outside the warm shed. The lose weight of experimental groups in basic ewe, reserved ewe and gimmer hogg were3.57, 2.98 and 2.74 kg less than that of the corresponding control group, and the weight gain of experimental groups in ram hogg was 3.08 kg higher than that of the control group during the warm shed experiment. The wool yield of experimental groups in basic ewe, reserved ewe and gimmer hogg were 0.22 kg（P〉0.05）, 0.11 kg（P〈0.05） and 0.59 kg（P〉0.05） higher than that of the control groups, respectively. The wool length of experimental groups in basic ewe, reserved ewe and gimmer hogg were 0.40 cm（P〉0.05）, 0.31 cm（P〉0.05） and 0.25 cm（P〉0.05） higher than that of the control groups, respectively. The wool fineness of experimental groups in basic ewe, reserved ewe and gimmer hogg were 0.01 μm（P〉0.05）, 0.09 μm（P〉0.05） and 0.24 μm（P〉0.05） higher than that of the control groups, respectively. The results indicated that warm shed feeding manner could effectively prevent the weight loss of Ordos fine wool sheep in cold season and increase the yield and length of wool and decrease the fineness of wool.

In this study, Kjeldahl method was used to determine the leaf crude protein content of Galium verum, Kochia prostrate and Chenopodium album. The results showed that the leaf crude protein content of Galium verum, Chenopodium album and Kochia prostrate were 0.58%, 1.06% and 0.40%, respectively. The crude protein content of Chenopodium album leaves was higher than that of Galium verum and Kochia prostrate leaves. While significant differences were found between the leaf crude protein content of Chenopodium album and Kochia prostrate（P〈0.05）, none of that were observed between Galium verum and Kochia prostrate and between Galium verum and Chenopodium album（P 〉0.05）.

A new kind of microbial fermentation protein feed was developed in present study and its effect on the production performance of Holstein cows was evaluated. A total of 30 Holstein cows were randomly divided into experimental group and control group, and there were 15 cows for each group.The diet composition of the control group was concentrate supplement, silage and alfalfa hay adding 30% linseed cake, and that of the experimental group was concentrate supplement, silage and alfalfa hay adding 30% microbial fermentation protein feed. The experimental period was 60 d. The results showed that compared with the control group, the average daily milk yield for each cow of the experimental group was increased by 2.25%（P〉0.05）, the total increasing amount of average milk production for each cow of the experimental group during the experiment was 43.51%（P〈0.01）higher than that of the control group,the concentrate/milk yield ratio of the experimental group was reduced by 9.20%（P〈0.05）,the average gross profit for each cow of the experimental group was 40.34 Yuan higher and the economic benefit of the experimental group was increased by 3.53%.

Feed determination technology is not only important means to guarantee the quality of feedstuffs and feed products in feed industry production,but also a significant basis for solution of trade disputes and administrative supervision. In this paper, the current situation and development of the feed determination techniques are reviewed with emphasis on the development and application of newly developed techniques in recent years.

Biochemistry, an important specialized fundamental course in biological sciences, is the foundation for learning professional courses. However,the contents of Biochemistry is complicated and abstract, and it is difficult to understand and master. Traditional teaching pattern of Biochemistry is unsuitable to cultivate comprehensive and high-quality talents. Therefore,the reform of teaching methods is imperative. In this paper, the teaching mode, teaching method and experiment teaching was discussed.

Target of the major of animal husbandry and veterinary medicine in the higher vocational colleges is to cultivate senior application talents who engage in front-line work of animal husbandry and veterinary medicine, such as the production, service and management. With the rapid development of modern animal husbandry, the higher comprehensive practical ability of animal husbandry and veterinary graduates are required by social employer. In this paper, the training model of animal husbandry and veterinary professional applied talents in new situation are discussed on the aspect of improving teachers′ quality, optimizing curriculum system, perfecting practice teaching, improving teaching content, establishing teaching resource database with professional characteristics and talent training evaluation system.

Veterinary Pathology, which is the ＂bridge course＂ between basic veterinary medicine and clinical veterinary medicine,is a basic course of veterinary medicine and focuses on pathogenesis of animal diseases. In recent years, in order to improve the practice ability of veterinary graduates, reforms on the teaching of Veterinary Pathology has been performed in Chinese universities and some progress has been made. However, there is still a gap between the actual teaching effect and expectation. In order to provide references for the teaching of Veterinary Pathology in China, the differences of the Veterinary Pathology teaching between Heilongjiang Bayi Agricultural University and Cornell University are taken as example to elaborate the differences of the Veterinary Pathology teaching between Chinese and American universities.

Curricula teaching are the key part of graduates′ cultivation. The significance of graduate′ curricula teaching is analyzed and the main problems existing in the curricula teaching are listed in this paper. A series of effective measures on teaching reform of Horticultural Crops Breeding and Superior Cultivars Propagation Science are also proposed to effectively improve training quality of professional degree graduates of horticultural majors.

Although the multi-media teaching is widely employed in colleges and universities, there are still some problems on its the application. Under the multi-media background, making best use of the advantages and bypassing the disadvantages of multimedia teaching method, combining the traditional teaching method with multi-media based on the features of varied majors and flexible using varied teaching methods are effective means to improve teaching quality.

Based on the teaching features of Pharmacology, the PBL combining with the case teaching method is tried to apply in Pharmacology for Nursing Care teaching. In order to further improve the teaching effect of Pharmacology for Nursing Care, the specific steps to combine PBL and case teaching method and the existing problems are elaborated and analyzed in this paper.

Based on the questionnaire investigation, the current situation of Animal Parasitic Diseases Prevention Experiment learning of veterinary students is analyzed on the aspect of learning interest, learning goals, experiment preparation situation,experiment operation and experiment report finish situation, and the improvement measures and suggestions are also put forward aiming at the existing problems.

Training the self-regulated learning ability of the college students is one of the important ways to construct of the learning society, and the self-regulated learning in the ubiquitous environment has become the important development direction of the next generation digital learning. In this paper, the construction strategy of the self-regulated learning knowledge resources systems of veterinary students in the ubiquitous environment are investigated, and specific suggestions are also proposed.

In order to strengthen the training of innovative pharmaceutical talents and to improve students′ comprehensive quality,the current situation, existing problems and prospect of teaching reform of pharmacy major experiment and practice were discussed on the aspect of practice teaching system, teaching content, and teaching method of innovative pharmacy major.

The advantages of RM6240 multi-channel physiology signal collection and processing system which is a kind of new generation medical laboratory equipment, including simple and convenient to operate, free to expansion and completely open, are introduced in this paper. The application of this device will be helpful for the functional experiment, and it has now become a useful auxiliary tool in functional experiment teaching.

The aim of this study was to investigate the diagnosis and treatment methods for canine pyometra. A total of 20 clinical cases of canine pyometra collected from Hefei Animal Health Centre and Chizhou Beilekang Pet Hospital were included in this study. The clinical symptoms, laboratory tests and instruments test were analyzed to explore the most effective diagnosis and treatment methods for canine pyometra. The results showed that the blood routine inspection, blood biochemical inspection, type-B ultrasonic inspection and X-ray inspection were of great significance for the diagnosis of canine pyometra. Furthermore, it was better to take the ovary and uterus excision surgery as soon as possible to prevent and cure canine pyometra.

In this paper, the clinical symptom and treatment protocol of an incidence of calcium deficiency symptom of young Panthera tigris altaica in Qinhuangdao Safari Park were introduced, and the causes of the disease and the rationality of pure meat diet of Panthera tigris altaica were discussed.