畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (1): 109-113.doi: 10.12160/j.issn.1672-5190.2025.01.014

• 动物疾病防控 • 上一篇    下一篇

云南省玉溪市羊棘球蚴病血清抗体调查及分析

李, 晶1, 吕嵘1, 宋诗雅1, 杨秋楠1, 朱禹坤1, 张晓舟1, 杨曦1, 吴明伟1, 罗晓燕1, 徐聪2   

  1. 1.玉溪市动物卫生监督与疫病预防控制中心,云南 玉溪 653100;
    2.玉溪市畜牧渔业发展服务中心,云南 玉溪 653100
  • 收稿日期:2024-07-05 出版日期:2025-01-30 发布日期:2025-04-03
  • 通讯作者: 徐聪(1981—),男,高级兽医师,硕士,主要从事动物疫病防控及流行病学调查工作。罗晓燕(1982—),女,高级兽医师,硕士,主要从事动物疫病实验室诊断及流行病学调查工作。
  • 作者简介:李晶(1988—),女,高级兽医师,硕士研究生,主要从事动物疫病实验室诊断及流行病学调查工作。
  • 基金资助:
    国家自然科学基金项目(32260889); 云南省国际科技特派员项目(202403AK140020); 云南省科技特派员项目(202204BK090769)

Serological Survey and Analysis of Echinococcosis in Sheep in Yuxi City, Yunnan Province

LI Jing1, LYU Rong1, SONG Shiya1, YANG Qiunan1, ZHU Yukun1, ZHANG Xiaozhou1, YANG Xi1, WU Mingwei1, LUO Xiaoyan1, XU Cong2   

  1. 1. Yuxi Center for Animal Health Supervision and Disease Control, Yuxi 653100, China;
    2. Yuxi Animal Husbandry and Fishery Development Service Center, Yuxi 653100, China
  • Received:2024-07-05 Online:2025-01-30 Published:2025-04-03

摘要: [目的]了解云南省玉溪市羊棘球蚴病的流行情况,为该地区科学防控羊棘球蚴病提供参考。[方法]采集玉溪市江川区、澄江市、华宁县、易门县、峨山彝族自治县、元江哈尼族彝族傣族自治县6个区(市、县及民族自治县)非免疫羊血清304份,利用ELISA方法检测棘球蚴病血清抗体,分析不同采样地点、饲养规模、年龄段、饲养模式、品种、性别羊棘球蚴病血清抗体阳性率的统计学差异。[结果]在304份羊血清样品中,检出阳性样品14份,总抗体阳性率为4.61%。江川区羊棘球蚴病血清抗体阳性率最高(7.50%),澄江市羊棘球蚴病血清抗体阳性率(0)最低,不同区(市、县及民族自治县)羊棘球蚴病血清抗体阳性率差异不显著(P>0.05);散养羊血清抗体阳性率(7.14%)高于规模场羊阳性率(2.00%),差异显著(P<0.05);≥3岁羊血清抗体阳性率最高(8.57%),≤1岁羊阳性率最低(0.95%),不同年龄段羊血清抗体阳性率差异显著(P<0.05);小群放牧羊血清抗体阳性率最高(8.06%),舍饲羊阳性率最低(1.20%),不同饲养模式羊血清抗体阳性率差异显著(P<0.05);绵羊血清抗体阳性率(9.41%)高于本地山羊阳性率(2.74%),二者差异显著(P<0.05);公羊血清抗体阳性率(6.00%)略高于母羊阳性率(4.33%),二者差异不显著(P>0.05)。[结论]参与调查的玉溪市江川区、澄江市、华宁县、易门县、峨山彝族自治县、元江哈尼族彝族傣族自治县6个区(市、县及民族自治县)中除澄江市未检出阳性样本,其余5个区(市、县及民族自治县)均存在羊棘球蚴感染,散养羊、高年龄段羊、小群放牧羊和绵羊的棘球蚴感染率较高。应采取综合防控措施,有效降低羊棘球蚴感染率。

关键词: 玉溪市, 羊, 棘球蚴病, ELISA检测, 阳性率, 血清抗体

Abstract: [Objective] To investigate the prevalence of echinococcosis in sheep in Yuxi City, Yunnan Province, to provide a scientific basis for its prevention and control in the region. [Methods] A total of 304 serum samples from non-immunized sheep were collected from six districts, cities, counties, or ethnic autonomous counties in Yuxi City, including Jiangchuan District, Chengjiang City, Huaning County, Yimen County, Eshan Yi Autonomous County, and Yuanjiang Hani, Yi and Dai Autonomous County. The enzyme-linked immunosorbent assay (ELISA) was employed to detect serum antibodies against Echinococcus spp., and statistical differences in antibody positivity rates were analyzed across various factors, including sampling locations, farming scales, age groups, rearing modes, breeds, and sexes. [Results] Of the 304 sheep serum samples, 14 tested positive, yielding an overall antibody positivity rate of 4.61%. Jiangchuan District exhibited the highest positivity rate (7.50%), while Chengjiang City showed the lowest (0), with no significant differences in positivity rates among the districts, cities, counties, and autonomous counties (P>0.05). The positivity rate in free-range sheep (7.14%) was higher than that in large-scale farm sheep (2.00%), with significant differences (P<0.05). Sheep aged ≥3 years had the highest positivity rate (8.57%), while those aged ≤1 year had the lowest (0.95%), with significant differences across age groups (P<0.05). Small-scale grazing sheep showed the highest positivity rate (8.06%), while barn-fed sheep had the lowest (1.20%), with significant differences among rearing modes (P<0.05). Sheep exhibited a higher positivity rate (9.41%) than local goats (2.74%), with a significant difference between breeds (P<0.05). The positivity rate in male sheep (6.00%) was slightly higher than in female sheep (4.33%), though the difference was not significant (P>0.05). [Conclusion] With the exception of Chengjiang City, where no positive samples were detected, echinococcosis infection was confirmed in sheep across the remaining five districts, cities, counties, or autonomous counties of Yuxi City surveyed (Jiangchuan District, Huaning County, Yimen County, Eshan Yi Autonomous County, and Yuanjiang Hani, Yi and Dai Autonomous County). Higher infection rates were observed in free-range sheep, older sheep (≥3 years), small-scale grazing sheep, and wool sheep. Comprehensive prevention and control measures should be implemented in this region to effectively reduce the incidence of sheep echinococcosis.

Key words: Yuxi City, sheep, echinococcosis, ELISA detection, positivity rate, serum antibodies

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