外源AsA引发对劣变燕麦种子活力的影响

doi:10.12160/j.issn.1672-5190.2024.06.013

摘要/Abstract

摘要： [目的] 探讨外源抗坏血酸（ascorbic acid,AsA）引发对劣变燕麦（Avena sativa）种子活力的影响,明确外源AsA引发对提高劣变燕麦种子活力的最佳浓度和时间,为燕麦饲草生产实践提供科学依据。[方法] 以控制劣变的燕麦种子为试验材料,经不同浓度（0、0.50、1.00、1.50、2.00、2.50 mmol/L）的AsA溶液引发0（CK）、4、8、12、16、20、24 h,分析其发芽率（germination percentage,Gp）、发芽指数（germination index,Gi）、幼苗活力指数（seedlings vigor index,SVI）和平均发芽时间（mean germination time,MGT）的变化规律。[结果] 外源AsA引发时间和浓度对劣变燕麦种子Gp、Gi和SVI有极显著（P<0.01）影响,对MGT无显著（P>0.05）影响;二者的交互作用对劣变燕麦种子SVI有极显著（P<0.01）影响,对Gp、Gi和MGT均无显著（P>0.05）影响。AsA浓度为0.5 mmol/L引发24 h时,劣变燕麦种子的Gp、Gi、SVI最高,MGT最短,Gp、Gi和SVI分别提高了23.42%、55.08%、41.42%,MGT缩短了14.25%。[结论] 外源AsA引发浓度、引发时间及二者的交互作用与劣变燕麦种子Gp、Gi、SVI和MGT变化均有密切关系,外源AsA浓度为0.5 mmol/L引发24 h是提高劣变燕麦种子活力的最佳处理。

关键词: 燕麦, 种子活力, 抗坏血酸, 种子劣变, 种子引发

Abstract: [Objective] The aim of this study was to assess the effect of exogenous ascorbic acid （AsA） priming on the seed vigor of deteriorated Avena sativa. At the same time, the optimal concentration and time of ascorbic acid （AsA） priming on seed vigor of Avena sativa was clarified, so as to provide a scientific basis for Avena sativa forage production practice. [Method] Avena sativa seeds with controlled deterioration were used as experimental materials, which were triggered by different concentrations of AsA solution （0, 0.50, 1.00, 1.50, 2.00, 2.50 mmol/L） for 0 （CK）, 4, 8, 12, 16, 20 and 24 h. Subsequently, the changes of germination percentage （Gp）, germination index （Gi）, seedlings vigor index （SVI）, and mean germination time （MGT） were analyzed. [Result] The priming time and concentration of exogenous AsA possessed highly significant （P<0.01） effects on the Gp, Gi and SVI of the deteriorated Avena sativa seeds, and had no significant （P>0.05） effects on the MGT. The interaction of the two had highly significant （P<0.01） effects on SVI and no significant （P>0.05） effects on Gp, Gi and MGT. The highest Gp, Gi and SVI and the shortest MGT were observed in the deteriorated Avena sativa seeds triggered by the AsA concentration of 0.5 mmol/L for 24 h. The Gp, Gi and SVI were increased by 23.42%, 55.08% and 41.42%, respectively, and the MGT was shortened by 14.25%. [Conclusion] The exogenous AsA priming concentration, priming time and the interaction between the two were closely related to the changes of Gp, Gi, SVI and MGT in deteriorated Avena sativa seeds. The exogenous AsA concentration of 0.5 mmol/L for 24 h was the best treatment to improve the vigor of deteriorated Avena sativa seeds.

Key words: Avena sativa, seed vigor, ascorbic acid, seed deterioration, seed priming

中图分类号:

S543.704.1

董秋丽, 王晶莹, 夏方山, 王聪聪, 李尹琳, 张杰敏, 李红玉. 外源AsA引发对劣变燕麦种子活力的影响[J]. 畜牧与饲料科学, 2024, 45(6): 87-92.

Dong Qiuli, Wang Jingying, Xia Fangshan, Wang Congcong, Li Yinlin, Zhang Jiemin, Li Hongyu. Effect of Exogenous AsA Priming on Seed Vigor of Deteriorated Avena sativa Seeds[J]. Animal Husbandry and Feed Science, 2024, 45(6): 87-92.

参考文献

[1] 毛培胜,欧成明,贾志程,等.我国牧草与草坪草种子生产技术的研究进展[J].中国草地学报,2023,45(1):1-11.
[2] 丁忆然,李卫华,柴亚茹,等.人工老化和修复处理对小麦种子活力及生理特性的影响[J].种子,2016,35(4):13-16,21.
[3] 李如雪. 小麦种子活力状况分析与种子活力评价技术研究[D].泰安:山东农业大学,2020.
[4] 米春娇,洪流,马馼,等.谷胱甘肽引发对老化燕麦种子发芽与幼苗生长特性的影响[J].草地学报,2024,32(3):928-934.
[5] 邓凤玲,李春生,顾建伟.微量元素在油菜种子引发中的调控作用及实用性[J].湖北农业科学,2024,63(6):5-11,21.
[6] 刘小越,兴旺,刘大丽,等.苹果酸对甜菜种子的活力影响研究[J].中国农学通报,2022,38(12):33-40.
[7] 宋建超,杨才艳,杨航,等.外源2,4-表油菜素内酯对NaCl胁迫下垂穗披碱草种子萌发及幼苗生理特性的影响[J].草原与草坪,2023,43(6):121-129.
[8] 王勃,王聪聪,夏方山,等.硒引发对不同品种紫花苜蓿种子抗氧化特性的影响[J].草地学报,2022,30(8):2037-2044.
[9] EL-HAWARY M M,HASHEM O S M,HASANUZZAMAN M.Seed priming and foliar application with ascorbic acid and salicylic acid mitigate salt stress in wheat[J].Agronomy,2023,13(2):493.
[10] 戴茜. 抗坏血酸浸种提高皖麻一号抗旱性研究及抗旱种衣剂研制[D].武汉:华中农业大学,2024.
[11] 李艺寒,陈玉童,刘芯邑,等.外源抗坏血酸对棉花种子萌发耐冷性的影响[J].种子,2019,38(3):77-80.
[12] 夏方山,王雅聪,董秋丽,等.抗坏血酸引发对Na₂SO₄胁迫下燕麦种子活力的影响[J].山西农业大学学报(自然科学版),2019,39(2):1-6.
[13] 李红玉,王聪聪,夏方山,等.抗坏血酸引发对燕麦老化种子胚细胞抗氧化性能的影响[J].草地学报,2023,31(8):2392-2398.
[14] 钱文武,黄志超,朱慧森,等.KNO₃和抗坏血酸对老化草地早熟禾种子活力的影响[J].草地学报,2022,30(1):69-75.
[15] 孙铭,王思琪,艾尔肯·达吾提,等.抗氧化剂引发对无芒雀麦老化种子发芽及幼苗生长的影响[J].草业学报,2019,28(11):105-113.
[16] 马海霞.抗坏血酸(AsA)对棉花耐冷性的影响及种子引发抗氧化机制研究[D].石河子:石河子大学,2023.
[17] 周青平,胡晓炜,汪辉,等.燕麦在维护国家粮食安全中的重要作用[J].草业学报,2024,33(10):171-182.
[18] 柴明娜,朱志强,路素香,等.燕麦幼苗对低氮胁迫响应的表型及转录组分析[J].中国草地学报,2024,46(7):1-10.
[19] 王勃,夏方山,董秋丽,等.外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响[J].草地学报,2023,31(5):1461-1468.
[20] XIA F,WANG C,LI Y,et al.Influence of priming with exogenous selenium on seed vigour of alfalfa (Medicago sativa L.)[J].Legume Research,2021,44(9):1124-1127.
[21] XIA F,CHENG H,CHEN L,et al.Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds[J].BMC Plant Biology,2020,20(1):104.
[22] 夏方山. 不同老化处理对燕麦种子线粒体结构及抗氧化系统的影响[D].北京:中国农业大学,2015.
[23] ISTA.International rules for seed testing.International seed testing association[S]. Bassersdorf:ISTA,2019.
[24] ABDUL-BAKI A A,ANDERSON J D.Vigor determination in soybean seed by multiple criteria[J].Crop Science,1973,13(6):630-633.
[25] ELLIS R H,OSEI-BONSU K,ROBERTS E H.The influence of genotype,temperature and moisture on seed longevity in chickpea,cowpea and soya bean[J].Annals of Botany,1982,50(1):69-82.
[26] 李尹琳,毕铭,夏方山,等.外源氮引发对白羊草种子萌发特性的影响[J].畜牧与饲料科学,2023,44(3):83-88.
[27] 王璐洋,胡华兵,王荣华,等.氯化钙对甜菜种子引发的影响[J].中国糖料,2024,46(2):78-83.
[28] 欧成明,赵美琦,孙铭,等.抗坏血酸和水杨酸丸衣对NaCl胁迫下紫花苜蓿种子发芽特性的影响[J].草业学报,2022,31(4):93-101.
[29] 田雪. 谷子种子活力的FTIR光谱研究[D].昆明:云南师范大学,2022.
[30] 李洋,李月,祁静静,等.披碱草种子引发最佳引发剂及引发条件的筛选[J].种子,2023,42(12):125-129.
[31] 张玉洁,张惠忠,王茂芊.焦亚硫酸钠对甜菜种子引发的影响[J].中国糖料,2024,46(3):39-44.
[32] 王聪聪. 抗坏血酸引发对燕麦种子生理特性及差异蛋白表达的影响[D].太谷:山西农业大学,2022.
[33] 徐恒恒,黎妮,刘树君,等.种子萌发及其调控的研究进展[J].作物学报,2014,40(7):1141-1156.
[34] 赵恬,柳旭,周文楠,等.超声波和抗坏血酸处理对老化梭梭种子萌发及幼苗生理特性的影响[J].草业科学,2019,36(5):1333-1343.
[35] 江绪文,李贺勤,王建华.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J].植物生理学报,2015,51(2):166-170.