Abstract: A pair specific primer were designed and synthesized according to the published GIF（GM-CSF inhibitory factor） sequence of NZ2 strain in GenBank.Total DNA of the contagious ecthyma virus（CEV） strain（OV/nm-05） which was isolated in Inner Mongolia was extracted,and the GIF gene was amplificated.The GIF gene was cloned into pEASY-T1 plasmids and then sequenced.The acquired GIF gene sequences of OV/nm-05 was compared with those of 7 reference strains like OV-NZ2,OV-D1701,OV-IA82,OV-SA00,OV-F07.808R,OV-MUK59/05 and OV-F94.848R.Results showed that the GIF gene homology of OV/nm-05 with 7 reference strains was 95.7%,98.2%,96.7%,95.5%,95.9%,95.5% and 95.1%,respectively.