Abstract: True anestrus associated with ovarian dysfunction is one of the leading causes of reduced reproductive performance in female livestock,which has brought serious economic losses to livestock production.Numerous studies have proved that the traditional Chinese herbal medicines have obvious effects in prevention and treatment of animal diseases and have only minor side effects.‘Cuiqingsan’,a commonly used traditional Chinese herbal compound in veterinary clinic,has been demonstrated to have a promoting effect on estrus and ovulation and a therapeutic effect on true anestrus with inactive ovaries in female livestock.In this paper,the main components of ‘Cuiqingsan’ were introduced,and their corresponding pharmacodynamics were characterized.In addition,the research progress on application of ‘Cuiqingsan’ in therapy of true anestrus in female livestock and its underlining mechanisms were reviewed,in hoping to provide theoretical basis for its scientific and rational use in livestock production.