性控精液对奶牛体内胚胎质量、发育和移植妊娠率的影响

doi:10.12160/j.issn.1672-5190.2023.01.011

Abstract

Abstract:

[Objective] The present study aimed to assess the effects of sex-sorted semen on quality and development of embryo in vivo as well as on the pregnancy rate of embryo transfer in dairy heifers. [Method] A total of 144 dairy heifers served as donors were randomly assigned into a control group （n=63） or an experimental group （n=81）. Follicle stimulating hormone （FSH） was used for superovulation treatment at dosage of 260 mg per heifer. The dairy heifers in the control group and the experimental group were artificially inseminated with normal and sex-sorted semen, respectively. The obtained fresh in vivo-derived sex-sorted embryos were transferred into the recipients. The embryo production, embryo quality, embryo development, and pregnancy rate of embryo transfer were statistically recorded. [Result] The mean number of transferable embryos obtained from the donors in the experimental group （5.67） was significantly （P<0.05） lower than that in the control group （6.92）. The proportions of A-grade embryos （62.53%） and B-grade embryos （35.29%） in the transferable embryos obtained from the donors in the experimental group did not significantly （P>0.05） differ from those in the control group （66.51% of A-grade embryos and 30.97% of B-grade embryos). The donors in the experimental group had a significantly （P<0.05） higher proportion of morula （84.10%） in the transferable embryos in comparison to the control group （61.24%）, while had a significantly （P<0.05） lower proportion of blastocyst （15.90%） compared with the control group （38.76%）. The pregnancy rate of fresh embryo transfer in the experimental group （52.41%） was significantly （P<0.05） lower than that in the control group （66.13%）. [Conclusion] Compared with the use of normal semen and embryo, the use of sex-sorted semen lowered the mean number of transferable embryos and the proportion of blastocyst in the transferable embryos of the donors, and that of sex-sorted embryo decreased the pregnancy rate of the recipients. However, the quality of transferable embryos had no significant reduction. Optimizing the use of sex-sorted semen and the embryo transfer technique can improve the efficiencies of in vivo sex-sorted embryo production and embryo transfer.

Key words: dairy heifers, sex-sorted semen, superovulation, embryo transfer

CLC Number:

S823.913.6

HAO Hai-sheng, DU Wei-hua, PANG Yun-wei, ZOU Hui-ying, ZHAO Xue-ming, ZHAO Shan-jiang, ZHU Hua-bin. Effects of Sex-sorted Semen on In Vivo Embryo Quality，Development and Pregnancy Rate of Embryo Transfer in Dairy Heifers[J]. Animal Husbandry and Feed Science, 2023, 44(1): 71-75.

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Effects of Sex-sorted Semen on In Vivo Embryo Quality，Development and Pregnancy Rate of Embryo Transfer in Dairy Heifers

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