Abstract: The protection of baicalin on the acute liver injury in mice was discussed. Fifty mice were divided into 5 groups of the control, model, low, moderate and high baicalin doses randomly. The baicalin groups were given different doses of baicalin injection by subcutaneous injection and the acute liver injury was induced by carbon tetraehloride in mice, then the feed intake, drinking amount, liver index, activities of ALT in serum and liver and the content of TP, ALB and GLO of mice were observed. The results indicated that compared with model group, the activities of ALT in mice serum of all the close groups of baicalin were decreased extremely significantly （P〈0.01）, that in mice liver were increased extremely significantly （P〈0.01）and their other indices had no significant difference. It was concluded that the baicalin given by subcutaneous injection had some protection on the liver injury induced by carbon tetrachloride in mice and the moderate dose was best.