Abstract: PCR amplification in now strain HC-R of potato wore performed by using 14 screened and suitable ISSR primers, a total of 80 clear and stable bands were detected, which the amplification fragment range from 200 bp to 2 000 bp, and 5.7 bands could be avoragoly amplified with each primer, the percentage of polymorphic bands was up to 100%. Based on the amplification results of 14 ISSR primers, a DNA fingerprint map of HC-R was constructed, digitizing the polymorphic loci screened by each primer reasonable in turn, a 14 bit string：A4694724319A83 was obtained, the bit string could be uniquely identified HC-R as a molecular identity card. The molecular ID can be registered for the next now strain breeding, protecting intellectual property and providing molecular basis.