Abstract:

【Objective】To address the demands of large-scale planting of Xanthoceras sorbifolia,a large-scale regeneration system using tissue culture seedlings of the plant was constructed.【Methods】The explants of Xanthoceras sorbifolia were induced by tissue culture using the stem segments of the plant as experimental materials. The effects of various hormone doses on the induction,proliferation,and rooting of adventitious buds were examined to determine the optimal medium for Xanthoceras sorbifolia tissue culture.【Results】For adventitious bud induction,MS+0.10 mg/L TDZ+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC(activated carbon)medium had the highest induction rate of 68.57%. MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA₃+1.0 g/L Ca(NO₃)₂ medium had the highest proliferation coefficient of 3.20. Rooting medium 1/2MS+0.5 mg/L IBA had the highest rooting rate of 65.56%. Equal proportion mixture of perlite,pine needle soil and pastoral soil as the transplanting medium significantly increased the survival rate of sorbent seedlings,which was 88.75%.【Conclusion】The optimal adventitious bud medium was MS+0.10 mg/L TDZ+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC. The optimal proliferation medium was MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA₃+1.0 g/L Ca(NO₃)₂. The optimal rooting medium was 1/2MS+0.5 mg/L IBA. And the optimal transplanting medium was equal proportion mixture of perlite,pine needle soil and pastoral soil.

Key words: Xanthoceras sorbifolia, Explant, Tissue culture, Regeneration system