畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (1): 104-108.doi: 10.12160/j.issn.1672-5190.2025.01.013

• 动物疾病防控 • 上一篇    下一篇

聊城市阳谷县鸡传染性支气管炎病毒感染情况调查及S1基因遗传进化分析

刘庆伦1, 刘高升2   

  1. 1.阳谷县农业技术推广服务中心,山东 阳谷 252300;
    2.聊城大学农学院,山东 聊城 252000
  • 收稿日期:2024-10-21 出版日期:2025-01-30 发布日期:2025-04-03
  • 通讯作者: 刘高升(1980—),男,教授,博士,主要研究方向为动物分子生物学。
  • 作者简介:刘庆伦(1970—),男,高级农艺师,主要从事动物疫病防控技术推广工作。
  • 基金资助:
    聊城大学畜牧学学科开放课题(319462207-24); 阳谷县农业科技创新服务团队项目(P3193121

Survey of Avian Infectious Bronchitis Virus Infection in Yanggu County of Liaocheng City and Genetic Evolution Analysis of S1 Gene

LIU Qinglun1, LIU Gaosheng2   

  1. 1. Agricultural Technology Extension Service Centre of Yanggu County, Yanggu 252300, China;
    2. College of Agriculture, Liaocheng University, Liaocheng 252000, China
  • Received:2024-10-21 Online:2025-01-30 Published:2025-04-03

摘要: [目的]了解山东省聊城市阳谷县鸡传染性支气管炎病毒(infectious bronchitis virus,IBV)的流行情况和基因型特点。[方法]2022—2023年从阳谷县养殖量较大的4个乡镇的27个养鸡场采集病料样品266份,应用RT-qPCR法进行IBV检测,然后利用RT-PCR法扩增部分阳性样品的S1基因序列,分析阳性样本基因型和遗传变异情况。[结果]4个乡镇的养鸡场均存在IBV感染,从266份病料样品中检出42份IBV核酸阳性样品,养殖场阳性率和样品阳性率分别为51.85%(14/27)和15.79%(42/266);规模化蛋鸡场、规模化肉鸡场和散养鸡场样品中,以散养鸡场阳性率和样品阳性率最高,分别为57.14%(8/14)和21.30%(23/108);春季和冬季样品的IBV检出率较高,分别为24.53%(26/106)和12.05%(10/83);1月龄以下鸡群样品的阳性率最高,为33.75%(27/80)。对7份IBV阳性样品S1基因序列进行扩增、测序和分析,发现5份属于GI-19谱系,2份属于GI-22谱系。[结论]阳谷县存在IBV流行,春季和冬季为感染高发期,1月龄以下鸡群和散养鸡群感染风险较高,GI-19和GI-22为该地区IBV的优势谱系。

关键词: 鸡传染性支气管炎病毒, 流行情况, S1基因, 基因型

Abstract: [Objective] To understand the prevalence and genotypic characteristics of infectious bronchitis virus (IBV) in chickens in Yanggu County, Liaocheng City, Shandong Province. [Method] 266 disease samples were collected from 27 chicken farms in four townships with large breeding volume in Yanggu County from 2022 to 2023. RT-qPCR was applied to detect IBV, and then the S1 gene sequences of some positive samples were amplified using RT-PCR to analyze the genotypes and genetic variation. [Result] The study found that IBV infection was present in chicken farms in all four townships. Forty-two IBV nucleic acid-positive samples were detected from 266 diseased samples, and the positive rate of farms and positive rate of samples were 51.85% (14/27) and 15.79% (42/266), respectively. Among the samples from large-scale egg farms, large-scale broiler farms and free-range chicken farms, the highest positive rate and sample positivity rate were found in free-range chicken farms, which were 57.14% (8/14) and 21.30% (23/108), respectively. Spring and winter samples had higher IBV detection rates of 24.53% (26/106) and 12.05% (10/83), respectively. The highest positivity rate of 33.75% (27/80) was found in samples from chickens below 1 month of age. The S1 gene sequences of the seven IBV-positive samples were amplified, sequenced and analyzed, and it was found that five belonged to the GI-19 lineage and two to the GI-22 lineage. [Conclusion] There is an epidemic of IBV in Yanggu County, and spring and winter are the high infection periods. The risk of infection was higher in flocks under 1 month of age and free-range flocks, and GI-19 as well as GI-22 were the dominant lineages of IBV in this area.

Key words: infectious bronchitis virus in chickens, prevalence characteristics, S1 gene, genotypes

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