抗坏血酸引发对干旱胁迫下达乌里胡枝子种子活力的影响

doi:10.12160/j.issn.1672-5190.2025.06.007

摘要/Abstract

摘要： [目的] 探究干旱胁迫下抗坏血酸（ascorbic acid,AsA）引发对达乌里胡枝子（Lespedeza davurica）种子活力的影响。[方法] 以‘晋农1号’达乌里胡枝子种子为试验材料,使用浓度为15%的PEG-6000溶液模拟干旱胁迫,采用浓度为0.5、1.0、2.0、4.0和8.0 mmol/L的AsA溶液分别引发0（CK）、3、6、9和12 h后进行发芽试验,分析其发芽率（germination percentage,Gp）、发芽指数（germination index,Gi）、平均发芽时间（mean germination time,MGT）和幼苗活力指数（seedlings vigor index,SVI）的变化规律。[结果] 外源AsA引发提高了干旱胁迫下达乌里胡枝子的种子活力,引发浓度和时间与干旱胁迫下达乌里胡枝子种子Gp、Gi、SVI和MGT变化均有密切关系。AsA浓度为1.0 mmol/L引发9 h时,干旱胁迫下达乌里胡枝子种子的Gp、Gi、SVI最高,MGT最短,与CK相比Gp、Gi、SVI分别提高了200.00%、265.54%、508.33%,MGT缩短了16.74%。[结论] 外源AsA浓度为1.0 mmol/L,引发9 h对干旱胁迫下达乌里胡枝子种子活力的提升效果最好。

关键词: 达乌里胡枝子, 干旱胁迫, 抗坏血酸, 种子引发, 种子活力

Abstract: [Objective] To explore the effects of ascorbic acid （AsA） priming on the seed vigor of Lespedeza davurica under drought stress. [Methods] Taking the seeds of L. davurica ′Jinnong No.1′ as the test material, 15% PEG-6000 solution was used to simulate drought stress. The seeds were primed with AsA solutions at concentrations of 0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L for 0 （CK）, 3, 6, 9 and 12 hours respectively, followed by germination tests. The variation patterns of germination percentage （Gp）, germination index （Gi）, mean germination time （MGT） and seedling vigor index （SVI） were analyzed. [Results] Exogenous AsA priming improved the seed vigor of L. davurica under drought stress. Both priming concentration and duration were closely related to the changes in Gp, Gi, SVI and MGT of L. davurica seeds under drought stress. When the seeds were primed with 1.0 mmol/L AsA for 9 hours, the Gp, Gi and SVI of L. davurica seeds under drought stress reached the maximum values, while the MGT reached the minimum value. Compared with CK, Gp, Gi and SVI were increased by 200.00%, 265.54% and 508.33% respectively, and MGT was shortened by 16.74%. [Conclusion] Exogenous AsA at a concentration of 1.0 mmol/L with a priming duration of 9 h showed the best effect on improving the seed vigor of L. davurica under drought stress.

Key words: Lespedeza davurica, drought stress, ascorbic acid, seed priming, seed vigor

中图分类号:

S816
S541.5

王晶莹, 孙翔宇, 夏方山, 卫洋帆, 柴玲, 石宇艳. 抗坏血酸引发对干旱胁迫下达乌里胡枝子种子活力的影响[J]. 畜牧与饲料科学, 2025, 46(6): 45-52.

WANG Jingying, SUN Xiangyu, XIA Fangshan, WEI Yangfan, CHAI Ling, SHI Yuyan. Effects of Ascorbic Acid Priming on Seed Vigor of Lespedeza davurica under Drought Stress[J]. Animal Husbandry and Feed Science, 2025, 46(6): 45-52.

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