Abstract: [Objective] To evaluate the acute toxicity of Jieshitong tablet in mice, to observe its diuretic effect on healthy rats, and to assess its alleviating effect on oxalamide-induced renal calculi in rats. [Method] A total of 40 ICR mice （half females and half males） were selected and randomly divided into a control group and an administration group with 20 mice in each group. The weight changes and deaths of the mice before and after the administration of Jieshitong tablet were observed. In addition, a total of 36 SD rats （half females and half males） were divided into normal saline group （n=12）, low-dose administration group （n=12, 5% Jieshitong tablet solution） and high-dose administration group （n=12, 10% Jieshitong tablet solution）. The 24-hour urine output of the rats in each group was counted. Another 42 male SD rats were used to investigate the effect of Jieshitong tablet on body weights, kidney weights and kidney to weight ratio of rats after treatment of oxalamide （2% mixed feeding）. [Result] During the 7 consecutive days after the administration of Jieshitong tablet solution, there was no obvious abnormality in the physiological activities of the mice, and no significant （P>0.05） difference in body weights between the control group and the administration group was observed. Compared with the normal saline group, the urinary volumes of the two administration groups were significantly （P<0.01) increased within 24 hours after administration of Jieshitong tablet solution. Compared with the oxalamide-induced renal calculi model group, the number of crystals and calculi in the Jieshitong tablet administration groups was significantly （P<0.01 or P<0.001） reduced with the increase of administration dose, and the number of rats that formed renal calculi was significantly （P<0.01 or P<0.001） reduced. [Conclusion] Jieshitong tablet has no obvious acute toxicity to mice, has obvious inhibitive effect in rats, and has obvious inhibitive effect on oxalamide-induced renal calculi in rats.

Key words: Jieshitong tablet, acute toxicity, mice, oxalamide, renal calculi, rats