BGF生物有机肥对辣椒疫霉和茄病镰孢菌的抑菌效果

doi:10.12190/j.issn.2096-1197.2022.01.14

北方农业学报 ›› 2022, Vol. 50 ›› Issue (1): 101-106.doi: 10.12190/j.issn.2096-1197.2022.01.14

BGF生物有机肥对辣椒疫霉和茄病镰孢菌的抑菌效果

霍宏丽¹, 席先梅¹, 白全江¹, 妥德宝², 宋雪峰², 彭敏², 邱廷艳³, 刘丽茹⁴

1.内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特 010031;
2.内蒙古元泰丰（包头）生物科技有限公司,内蒙古萨拉齐 014100;
3.喀喇沁旗农牧技术推广中心植保站,内蒙古喀喇沁 024400;
4.乌海市海勃湾区乡村振兴建设服务中心,内蒙古海勃湾 016000

收稿日期:2021-08-03 出版日期:2022-02-20 发布日期:2022-04-11
通讯作者: 席先梅（1975—）,女,研究员,博士,主要从事植物病理的研究工作。
作者简介:霍宏丽（1991—）,女,助理研究员,硕士,主要从事植物病理的研究工作。
基金资助:
元泰丰（包头）生物科技有限公司资助项目; 国家重点研发计划项目（2018YFD0201205-5）

Antimicrobial activity of BGF bio-organic fertilizer against Phytophthora capsici and Fusarium solani

HUO Hongli¹, XI Xianmei¹, BAI Quanjiang¹, TUO Debao², SONG Xuefeng², PENG Min², QIU Tingyan³, LIU Liru⁴

1. Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Inner Mongolia Yuantaifeng（Baotou）Biotechnology Co.,Ltd.,Salaqi 014100,China;
3. Plant Protection Station of Agriculture and Animal Husbandry Technology Promotion Center in Harqin Banner,Harqin 024400,China;
4. Haibowan District Rural Revitalization Construction Service Center in Wuhai City,Haibowan 016000,China

Received:2021-08-03 Online:2022-02-20 Published:2022-04-11

摘要/Abstract

摘要： 【目的】评价BGF生物有机肥对辣椒疫病病原菌辣椒疫霉和辣椒根腐病病原菌茄病镰孢菌的抑菌效果。【方法】使用浓度梯度法稀释BGF生物有机肥,制成1、10、100、1 000、10 000 mg/L的马铃薯蔗糖琼脂（PDA）平板,以清水处理为对照,采用菌丝生长速率抑制法测定BGF生物有机肥对辣椒疫霉和茄病镰孢菌的抑菌率,通过毒力回归方程计算EC₅₀。【结果】10 000 mg/L浓度的BGF生物有机肥对辣椒疫霉的抑菌率最高,为74.93%;其次为1 000 mg/L的抑菌率,为56.27%。10 000 mg/L浓度的BGF生物有机肥对茄病镰孢菌的抑菌率最高,为78.54%;其次为1 000 mg/L的抑菌率,为63.52%。BGF生物有机肥对辣椒疫霉的EC₅₀为401.038 mg/L,对茄病镰孢菌的EC₅₀为390.077 mg/L。【结论】1 000~10 000 mg/L浓度的BGF生物有机肥对辣椒疫霉和茄病镰孢菌均有较好的抑制作用。

关键词: 辣椒, 生物有机肥, 辣椒疫霉, 茄病镰孢菌, 抑菌率, 毒力

Abstract: 【Objective】To study the antimicrobial activity of BGF bio-organic fertilizer on Phytophthora capsici and Fusarium solani.【Methods】BGF bio-organic fertilizer was diluted in a gradient of 1,10,100,1 000,and 10 000 mg/L in potato sucrose agar（PDA）plates,with water as control. The bacteriostatic rate of BGF bio-organic fertilizer on Phytophthora capsici and Fusarium solani was determined using the mycelial growth rate inhibition method,and the EC₅₀ was calculated using the virulence regression equation.【Results】The 10 000 mg/L BGF bio-organic fertilizer had the highest Phytophthora capsici inhibition rate,at 74.93%,followed by 1 000 mg/L,at 56.27%. The 10 000 mg/L BGF bio-organic fertilizer had the highest inhibition rate against Fusarium solani,at 78.54%. At 63.52%,the rate of 1 000 mg/L was the second highest. To Phytophthora capsici and Fusarium solani,the EC₅₀ of BGF bio-organic fertilizer was 401.038 mg/L and 390.077 mg/L,respectively.【Conclusion】Phytophthora capsici and Fusarium solani were both inhibited by BGF bio-organic fertilizer at concentrations of 1 000-10 000 mg/L.

Key words: Capsicum, Biological organic fertilizer, Phytophthora capsici, Fusarium solani, Bacteriostatic rate, Toxicity

中图分类号:

S482.4

霍宏丽, 席先梅, 白全江, 妥德宝, 宋雪峰, 彭敏, 邱廷艳, 刘丽茹. BGF生物有机肥对辣椒疫霉和茄病镰孢菌的抑菌效果[J]. 北方农业学报, 2022, 50(1): 101-106.

HUO Hongli, XI Xianmei, BAI Quanjiang, TUO Debao, SONG Xuefeng, PENG Min, QIU Tingyan, LIU Liru. Antimicrobial activity of BGF bio-organic fertilizer against Phytophthora capsici and Fusarium solani[J]. Journal of Northern Agriculture, 2022, 50(1): 101-106.

参考文献

[1] 张化霜. 辣椒疫霉对杀菌剂抗药性研究进展[J].农药研究与应用,2011,15(3):11-14.
[2] 李萍,江涛,高智谋,等.辣椒疫霉(Phytophthora capsici)对辣椒的致病力分化研究[J].植物病理学报,2012,42(4):431-435.
[3] 赵卫松,韩秀英,王文桥,等.辣椒疫霉对杀菌剂抗药性研究进展[J].农药,2010,49(2):86-89.
[4] 邓魁元. 露地辣椒疫病的发生与综合防治[J].现代农业科技,2005(2):17.
[5] 刘丽云,刘晓林,刘志恒,等.辣椒根腐病菌生物学特性研究[J].沈阳农业大学学报,2007,38(1):54-58.
[6] 蔡高磊,赵昌松,彭宣和,等.十堰辣椒根腐病病原菌分离鉴定及高氏15号的生物防治作用研究[J].华中师范大学学报(自然科学版),2019,53(2):248-254.
[7] RAGHU S,BENAGI V I,NARGUND V B.Cultural,morphological and pathogenic variability among the isolates of Fusarium solani causing wilt disease of chilli(Capsicum annuum L.)[J].Journal of Pure and Applied Microbiology,2016,10(1):599-603.
[8] NAIK M K,MADHUKAR H M,DEVIKA G S R.Evaluation of fungicides against Fusarium solani,the causal agent of wilt of chilli[J].Journal of Vegetation Science,2007,34(2):173-176.
[9] 丁万隆,王蓉,郝单丽,等.人参病害生防微生物菌剂的抑菌活性评价[J].中国现代中药,2018,20(9):1122-1125.
[10] 沈德龙,李俊,姜昕.我国生物有机肥的发展现状及展望[J].中国农技推广,2007,23(9):35-37.
[11] 任旭琴,陈伯清,彭莉,等.凹土和有益微生物菌液对淮安红椒连作土壤养分和酶活性的影响[J].江苏农业科学,2016,44(10):491-494.
[12] 吉沐祥,邬劼,王晓琳,等.叶菌唑与肟菌酯及其复配对葡萄炭疽病菌及白腐病菌的室内抑菌活性及田间防效[J].江苏农业科学,2019,47(19):98-102.
[13] 胡可,李华兴,卢维盛,等.生物有机肥对土壤微生物活性的影响[J].中国生态农业学报,2010,18(2):303-306.
[14] XU M G,TANG H J,YANG X Y,et al.Best soil managements from long-term field experiments for sustainable agriculture[J].Journal of Integrative Agriculture,2015,14(12):2401-2404.
[15] OELMANN Y,LANGE M,LEIMER S,et al.Above- and belowground biodiversity jointly tighten the P cycle in agricultural grasslands[J].Nature Communications,2021,12(1):4431.
[16] STAMFORD N P,SILVA E V N,OLIVEIRA W D S,et al.Organic matter inoculated with diazotrophic bacterium Beijerinckia indica and Cunninghamella elegans fungus containing chitosan on banana “Williams” in field[J].Acta Scientiarum.Agronomy,2017,39(1):33-41.
[17] STAMFORD N P,FELIX F,OLIVEIRA W,et al.Interactive effectiveness of microbial fertilizer enriched in N on lettuce growth and on characteristics of an ultisol of the rainforest region[J].Scientia Horticulturae,2019,247:242-246.
[18] LI R Y,PANG Z Q,ZHOU Y M,et al.Metagenomic analysis exploring taxonomic and functional diversity of soil microbial communities in sugarcane fields applied with organic fertilizer[J].BioMed Research International,2020:1-11.
[19] YE L,ZHAO X,BAO E C,et al.Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality[J].Scientific Reports,2020,10(1):177.
[20] ROBERTS D P,LOHRKE S M,MEYER S F,et al.Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber[J].Crop Protection,2005,24(2):141-155.
[21] 赵远征,王东,张翔宇,等.生物菌肥对马铃薯4种土传病害病原菌的室内抑制效果[J].北方农业学报,2021,49(2):78-83.
[22] 凌宁,王秋君,杨兴明,等.根际施用微生物有机肥防治连作西瓜枯萎病研究[J].植物营养与肥料学报,2009,15(5):1136-1141.
[23] 何欣,郝文雅,杨兴明,等.生物有机肥对香蕉植株生长和香蕉枯萎病防治的研究[J].植物营养与肥料学报,2010,16(4):978-985.
[24] 刘苹,张博,齐军山,等.生物有机肥对小麦根腐病的防效及其机理初探[J].麦类作物学报,2019,39(9):1132-1137.
[25] RAGAB M M,ASHOUR A M A,ABDEL-KADER M M,et al.In vitro evaluation of some fungicides alternatives against Fusarium oxysporum the causal of wilt disease of pepper(Capsicum annum L.)[J].International Journal of Agriculture and Forestry,2012,2(2):70-77.
[26] 陈建爱,周善跃,杨焕明,等.黄绿木霉T1010防治辣椒根腐病茄镰孢[J].农学学报,2012,2(10):14-18.
[27] JOO G J.Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii[J].Biotechnology Letters,2005,27(3):201-205.

BGF生物有机肥对辣椒疫霉和茄病镰孢菌的抑菌效果

Antimicrobial activity of BGF bio-organic fertilizer against Phytophthora capsici and Fusarium solani

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